Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model

  • Authors:
    • Bora Lee
    • Dae Young Lee
    • Ki Hyun Yoo
    • Nam In Baek
    • Jong-Hwa Park
    • In Sik Chung
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  • Published online on: April 24, 2012     https://doi.org/10.3892/ol.2012.693
  • Pages: 22-28
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Abstract

The aim of the present study was to investigate the cytotoxic effect of calenduloside E 6'-methyl ester (oleanolic acid 3-O-β-D-glucuronopyranoside‑6'-methyl ester) isolated from Acanthopanax sessiliflorus fruits was investigated in CT-26 mouse colon carcinoma cells. Calenduloside E 6'-methyl ester dose-dependently inhibited the viability of CT-26 cells. Apoptosis was characterized by the detection of annexin-V and sub-G1 apoptotic cell populations, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA fragmentation experiments. Results showed that the number of immunostained annexin-V-FITC and sub-G1 cells increased after treatment with calenduloside E 6'-methyl ester. Calenduloside E 6'-methyl ester also increased terminal deoxynucleotidyl transferase dUTP nick end labeled-CT-26 cells. It induced DNA fragmentation. and the cleavage of caspase-8, -9, -3 and poly ADP-ribose polymerases. In addition, calenduloside E 6'-methyl ester suppressed the volume and weight of tumors in BALB/c mice subcutaneously implanted with CT-26 cells. These results indicate that calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 carcinoma animal model.
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July 2012
Volume 4 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lee B, Lee DY, Yoo KH, Baek NI, Park J and Chung IS: Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model. Oncol Lett 4: 22-28, 2012
APA
Lee, B., Lee, D.Y., Yoo, K.H., Baek, N.I., Park, J., & Chung, I.S. (2012). Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model. Oncology Letters, 4, 22-28. https://doi.org/10.3892/ol.2012.693
MLA
Lee, B., Lee, D. Y., Yoo, K. H., Baek, N. I., Park, J., Chung, I. S."Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model". Oncology Letters 4.1 (2012): 22-28.
Chicago
Lee, B., Lee, D. Y., Yoo, K. H., Baek, N. I., Park, J., Chung, I. S."Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model". Oncology Letters 4, no. 1 (2012): 22-28. https://doi.org/10.3892/ol.2012.693