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Article

Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer

  • Authors:
    • Xin Min Xu
    • Jian Chang Qian
    • Zhou Lu Deng
    • Zhe Cai
    • Tao  Tang
    • Peng Wang
    • Ke Hua Zhang
    • Jian-Ping Cai
  • View Affiliations / Copyright

    Affiliations: Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China, Department of Pharmacology, Wenzhou Medical College, Zhejiang 325035, P.R. China, Department of General Surgery, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, P.R. China, Department of Immunology, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, P.R. China, The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, P.R. China
  • Pages: 339-345
    |
    Published online on: May 14, 2012
       https://doi.org/10.3892/ol.2012.714
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Abstract

The aim of this study was to determine the expression of miR-21, miR-31, miR-96 and miR-135b in 52 paired colorectal cancer (CRC) tissues and to analyze the correlation between microRNAs (miRNAs) and clinicopathological features. We developed a quantification method that relies on a standard plot, constructed from known concentrations of standards, in order to measure the number of miRNAs. In addition to this, we analyzed the expression levels of miR-21, miR-31, miR-96 and miR-135b in 52 cases of primary CRC and corresponding normal mucosal tissue using real-time PCR with SYBR-Green I. An independent sample t-test was used to compare the differential expression between tumor tissues and normal mucosal tissues. The Mann-Whitney U and Kruskall‑Wallis tests were used to compare the correlation between miRNA expression levels and clinicopathological features. The expression of miR-21, miR-31, miR-96 and miR‑135b was upregulated in the CRC tissues compared to normal mucosal tissues (P<0.05). Furthermore, miR-21 and miR-135b were positively correlated with the clinical stage (P=0.048 and P=0.029, respectively), while miR-96 and miR-135b were correlated with liver metastasis (P=0.006 and P=0.013, respectively). Our results suggest that miR-21, miR-31, miR-96 and miR-135b may function in the process of CRC development and progression. miR-135b levels in particular may correlate with the degree of malignancy.
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Copy and paste a formatted citation
Spandidos Publications style
Xu XM, Qian JC, Deng ZL, Cai Z, Tang T, Wang P, Zhang KH and Cai J: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer. Oncol Lett 4: 339-345, 2012.
APA
Xu, X.M., Qian, J.C., Deng, Z.L., Cai, Z., Tang, T., Wang, P. ... Cai, J. (2012). Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer. Oncology Letters, 4, 339-345. https://doi.org/10.3892/ol.2012.714
MLA
Xu, X. M., Qian, J. C., Deng, Z. L., Cai, Z., Tang, T., Wang, P., Zhang, K. H., Cai, J."Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer". Oncology Letters 4.2 (2012): 339-345.
Chicago
Xu, X. M., Qian, J. C., Deng, Z. L., Cai, Z., Tang, T., Wang, P., Zhang, K. H., Cai, J."Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer". Oncology Letters 4, no. 2 (2012): 339-345. https://doi.org/10.3892/ol.2012.714
Copy and paste a formatted citation
x
Spandidos Publications style
Xu XM, Qian JC, Deng ZL, Cai Z, Tang T, Wang P, Zhang KH and Cai J: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer. Oncol Lett 4: 339-345, 2012.
APA
Xu, X.M., Qian, J.C., Deng, Z.L., Cai, Z., Tang, T., Wang, P. ... Cai, J. (2012). Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer. Oncology Letters, 4, 339-345. https://doi.org/10.3892/ol.2012.714
MLA
Xu, X. M., Qian, J. C., Deng, Z. L., Cai, Z., Tang, T., Wang, P., Zhang, K. H., Cai, J."Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer". Oncology Letters 4.2 (2012): 339-345.
Chicago
Xu, X. M., Qian, J. C., Deng, Z. L., Cai, Z., Tang, T., Wang, P., Zhang, K. H., Cai, J."Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer". Oncology Letters 4, no. 2 (2012): 339-345. https://doi.org/10.3892/ol.2012.714
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