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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September 2012 Volume 4 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells

  • Authors:
    • Qinjun Wei
    • Wei He
    • Yajie Lu
    • Jun Yao
    • Xin Cao
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Nanjing Medical University, Nanjing 210029, P.R. China
  • Pages: 438-442
    |
    Published online on: June 7, 2012
       https://doi.org/10.3892/ol.2012.744
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Abstract

Inhibitor of growth 4 (ING4) is a member of the ING family and acts as a tumor suppressor protein. To investigate the impact of ING4 on breast cancer proliferation, the present study examined the antitumor effects caused by upregulation in the expression of ING4 and its possible mechanism of effect in MCF-7 cells. A plasmid-based expression system encoding the ING4 gene was used to construct a stable cell line and overexpress ING4 in MCF-7 cells. Real‑time PCR and western blot analysis were used to detect the mRNA and protein expression levels of ING4, respectively. Cell growth was examined by methylthiazolyltetrazolium (MTT) assay. Cell cycle distribution and cell apoptosis were measured by flow cytometry. The expression of p21, p53 and bax genes were tested by real-time PCR and western blot analysis. The stably transfected cell lines pcDNA3.1(+)/ING4 (with the ING4 gene) and pcDNA3.1(+) (an empty vector) were established. The expression levels of ING4 mRNA and protein in the stable cell line expressing pcDNA3.1(+)/ING4 were significantly higher than those of the two control cell lines. The cell proliferation of stably transfected cells was inhibited, and the inhibitory rate was 62.58±2.93%. Based on the changes in cell cycle distribution in stably transfected cells compared with two control cell lines, a number of cells were blocked in the G0/G1 phase 67.82±3.78% (P<0.05). In addition, the apoptotic rate was significantly higher, at 31.51±3.02% (P<0.05). Real-time PCR revealed that p21 and bax mRNA expression were increased (P<0.01), but the expression of p53 did not change significantly (P>0.05) in the stably transfected cell lines. Western blot analysis results of p21, bax and p53 were in accordance with real-time PCR results. ING4 upregulation may inhibit breast cancer cell proliferation and accelerate the process of apoptosis. It is suggested that ING4 plays a significant role in the suppression of breast cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Wei Q, He W, Lu Y, Yao J and Cao X: Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells. Oncol Lett 4: 438-442, 2012.
APA
Wei, Q., He, W., Lu, Y., Yao, J., & Cao, X. (2012). Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells. Oncology Letters, 4, 438-442. https://doi.org/10.3892/ol.2012.744
MLA
Wei, Q., He, W., Lu, Y., Yao, J., Cao, X."Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells". Oncology Letters 4.3 (2012): 438-442.
Chicago
Wei, Q., He, W., Lu, Y., Yao, J., Cao, X."Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells". Oncology Letters 4, no. 3 (2012): 438-442. https://doi.org/10.3892/ol.2012.744
Copy and paste a formatted citation
x
Spandidos Publications style
Wei Q, He W, Lu Y, Yao J and Cao X: Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells. Oncol Lett 4: 438-442, 2012.
APA
Wei, Q., He, W., Lu, Y., Yao, J., & Cao, X. (2012). Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells. Oncology Letters, 4, 438-442. https://doi.org/10.3892/ol.2012.744
MLA
Wei, Q., He, W., Lu, Y., Yao, J., Cao, X."Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells". Oncology Letters 4.3 (2012): 438-442.
Chicago
Wei, Q., He, W., Lu, Y., Yao, J., Cao, X."Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells". Oncology Letters 4, no. 3 (2012): 438-442. https://doi.org/10.3892/ol.2012.744
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