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Article

RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia

  • Authors:
    • Jiao Jian Tong
    • Zhang Yan
    • Ren Jian
    • Huang Tao
    • Ouyang Tao Hui
    • Chen Jian
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
  • Pages: 495-500
    |
    Published online on: June 27, 2012
       https://doi.org/10.3892/ol.2012.777
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Abstract

The purpose of this study was to investigate the mechanism of glioma cell invasion in hypoxic conditions. We demonstrated that hypoxia increased cell invasion, matrix metalloproteinase-2 (MMP2) activity and time-dependent expression of hypoxia inducible factor-1α (HIF‑1α) in human glioma cells. These data suggest that MMP2 may play a significant role in tumor invasion in hypoxic conditions. We investigated the mechanisms involved in the increased MMP2 activity and cell invasion in hypoxic conditions. Increased expression of phospho-Jun NH2-terminal kinase (p-JNK) and phospho-c-Jun (p-c-Jun) in glioma cells induced by hypoxia was detected. Furthermore, this effect may be reduced by inhibiting the JNK signaling pathway. We found that inhibition of RhoA geranylgeranylation by geranylgeranyltransferase inhibitor-2147 (GGTI-2147) or knockdown of RhoA by siRNA against RhoA reduced the expression of p-JNK and p-c-Jun, and decreased MMP2 activity and glioma cell invasion in hypoxic conditions. These data suggest a link among RhoA, JNK, c-Jun and MMP2 activity that is functionally involved in the increased glioma cell invasion induced by hypoxia.
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Copy and paste a formatted citation
Spandidos Publications style
Tong JJ, Yan Z, Jian R, Tao H, Hui OT and Jian C: RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia. Oncol Lett 4: 495-500, 2012.
APA
Tong, J.J., Yan, Z., Jian, R., Tao, H., Hui, O.T., & Jian, C. (2012). RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia. Oncology Letters, 4, 495-500. https://doi.org/10.3892/ol.2012.777
MLA
Tong, J. J., Yan, Z., Jian, R., Tao, H., Hui, O. T., Jian, C."RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia". Oncology Letters 4.3 (2012): 495-500.
Chicago
Tong, J. J., Yan, Z., Jian, R., Tao, H., Hui, O. T., Jian, C."RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia". Oncology Letters 4, no. 3 (2012): 495-500. https://doi.org/10.3892/ol.2012.777
Copy and paste a formatted citation
x
Spandidos Publications style
Tong JJ, Yan Z, Jian R, Tao H, Hui OT and Jian C: RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia. Oncol Lett 4: 495-500, 2012.
APA
Tong, J.J., Yan, Z., Jian, R., Tao, H., Hui, O.T., & Jian, C. (2012). RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia. Oncology Letters, 4, 495-500. https://doi.org/10.3892/ol.2012.777
MLA
Tong, J. J., Yan, Z., Jian, R., Tao, H., Hui, O. T., Jian, C."RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia". Oncology Letters 4.3 (2012): 495-500.
Chicago
Tong, J. J., Yan, Z., Jian, R., Tao, H., Hui, O. T., Jian, C."RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia". Oncology Letters 4, no. 3 (2012): 495-500. https://doi.org/10.3892/ol.2012.777
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