Increased plasma microRNA and CD133/CK18‑positive cancer cells in the pleural fluid of a pancreatic cancer patient with liver and pleural metastases and correlation with chemoresistance

Ren,Chuanli; Chen,Hui; Han,Chongxu; Wang,Daxin; Fu,Deyuan

doi:10.3892/ol.2012.798

October 2012 Volume 4 Issue 4

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October 2012 Volume 4 Issue 4

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Article

Increased plasma microRNA and CD133/CK18‑positive cancer cells in the pleural fluid of a pancreatic cancer patient with liver and pleural metastases and correlation with chemoresistance

Authors:
- Chuanli Ren
- Hui Chen
- Chongxu Han
- Daxin Wang
- Deyuan Fu
View Affiliations / Copyright

Affiliations: Medical Laboratory, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou 225001, P.R China, Geriatric Medicine Department, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou 225001, P.R China, Research Centre of Biomedical Engineering, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou 225001, P.R China, General Surgery Department, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou 225001, P.R China
Pages: 691-694
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Published online on: July 13, 2012

https://doi.org/10.3892/ol.2012.798
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Abstract

We report a case of notably increased plasma levels of microRNA (miR)‑21, miR‑25, miR‑103 and miR‑151 in a pancreatic cancer patient with liver and pleural metastases. CD45‑coated immunomagnetic beads detected an enrichment of malignant cancer cells in the pleural fluid, and CD133+CK18+ cancer cells were identified. Using computer tomography (CT) combined with cancer cells stained in the pleural fluid, a previously healthy 60‑year‑old male was diagnosed with pancreatic cancer with multiple liver tumor metastases. Cancer antigen 19‑9 (CA19‑9), alkaline phosphatase (ALP) and γ‑glutamate‑transpeptidase (γ‑GT) were notably increased in the serum, and carcinoembryonic antigen (CEA) was increased in the pleural fluid. The patient succumbed to the disease three months following standard chemotherapy. The increased levels of plasma miR‑21, miR‑25, miR‑103 and miR‑151, as well as the identification of CD133+CK18+ cells in the pleural fluid of a pancreatic cancer patient with liver metastases, may regulate the molecular mechanisms involved in chemoresistance. The patient was insensitive to chemotherapy and succumbed 3 months later. Full elucidation of the molecular and pathological features of pancreatic cancer may be a novel strategy for diagnosis and tailored therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Increased plasma microRNA and CD133/CK18‑positive cancer cells in the pleural fluid of a pancreatic cancer patient with liver and pleural metastases and correlation with chemoresistance

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