Cyclin A overexpression is associated with chemosensitivity to paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma

  • Authors:
    • Jun‑Xing Huang
    • Shuang‑Le Shen
    • Mei Lin
    • Wei Xiao
    • Wei‑Chang Chen
    • Mao‑Song Lin
    • Hong Yu
    • Ping Chen
    • Rong‑Yu Qian
  • View Affiliations

  • Published online on: July 20, 2012     https://doi.org/10.3892/ol.2012.814
  • Pages: 607-611
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Abstract

The aim of this study was to investigate the correlation between cyclin A expression and efficacy of paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma (ESCC). The expression of cyclin A was examined in 48 newly diagnosed ESCC patients prior to treatment using the MaxVision immunohistochemistry method. The patients received four cycles of paclitaxel‑based chemotherapy, the short‑term treatment efficacy was evaluated and a 3‑year follow‑up was conducted. The response rate was greater in patients with positive cyclin A expression compared with those with negative expression (54.8 vs. 23.5%; χ2=4.373; P<0.05). Univariate and multivariate Cox analysis revealed that clinicopathological stage, degree of differentiation and expression of cyclin A were independent prognosis factors in patients with ESCC following paclitaxel‑based chemotherapy. ESCC patients with positive cyclin A expression demonstrated an increased sensitivity to paclitaxel‑based chemotherapy, suggesting that cyclin A may be used as a marker to predict the treatment efficacy of paclitaxel in patients with ESCC.

Introduction

Esophageal squamous cell carcinoma (ESCC) remains the most common type of esophageal cancer worldwide. Even in resectable esophageal cancer, the 5-year survival rate remains only 14–45% (1,2). Treatment failure of ESCC is due to the high incidence of local-regional failure and early systemic dissemination of the disease (3). Chemotherapy is one of the most important treatment methods for advanced esophageal carcinoma. Several prospective and retrospective studies in patients with locally advanced thoracic esophageal cancers or in elderly patients with early stage esophageal cancer, particularly epidermoid, who respond to chemoradiotherapy, have suggested that there is no benefit from the addition of surgery following chemoradiotherapy compared with the continuation of additional chemoradiotherapy. Following chemoradiotherapy, with salvage therapy if required, treatment outcomes among patients with resectable thoracic ESCC were comparable or superior to the treatment outcomes following surgery alone (47). Therefore, the role of chemotherapy is becoming increasingly important in the treatment of ESCC.

Patients with ESCC demonstrate different sensitivity for chemotherapy. Therefore, in order to improve the prognosis of esophageal carcinoma, there is an urgent requirement to predict the sensitivity of chemotherapy drugs and tailor individual treatment. Paclitaxel is one of the most effective drugs for esophageal carcinoma treatment as it targets cells in the G2/M phase transition. As a critical regulatory protein, cyclin A may promote G2/M phase transition and mitosis (8). Our previous studies have revealed that overexpression of cyclin A in ESCC is associated with cell proliferation and survival (911). As the clinical application of immunohistochemistry is relatively cost-effective (2), in this study, we explore the possibility of evaluating paclitaxel efficacy by examining the correlation between cyclin A expression and efficacy of paclitaxel-based chemotherapy in patients with ESCC.

Patients and methods

Patients and specimens

All specimens collected for this retrospective study were obtained from patients who had not received chemotherapy or radiotherapy prior to biopsy. A total of 48 ESCC patients were diagnosed by endoscopic biopsy pathology between 2006 and 2009 at The People’s Hospital of Taizhou (Taizhou, Jiangsu, China). Those deemed unsuitable for surgery based on their performance status, bulky local disease, Karnofsky score (≥70 points) or personal choice received chemotherapy. Of the 48 patients, 33 were male and 15 were female. The age of the patients ranged from 47 to 78 years and the median age was 58 years. A total of 16, 19 and 13 cases were upper, middle and lower thoracic ESCC, respectively. Staging was determined according to the American Joint Committee on Cancer tumor-node-metastasis (TNM) classification (12). A total of 7, 13 and 28 cases were stage I–II, III and IV, respectively; while 10 cases from normal esophageal mucosa were used as a control group. With regards to cancer cell differentiation, 10, 16 and 22 cases were well-, moderately and poorly differentiated, respectively.

Treatment methods

Paclitaxel (180 mg/m2) was infused on day 1 and cisplatin (20 mg/m2) was infused on days 1–3 of a 21-day cycle, yielding a total of 4 chemotherapy cycles. The patients were also administered conventional anti-allergy pretreatment prior to chemotherapy.

Short-term efficacy evaluation

All patients received an endoscopy examination, esophageal barium X-ray and a neck and chest computed tomography (CT) scan prior to treatment and 1 month following treatment. The evaluation of the response rate was based on WHO treatment efficacy evaluation criteria for solid tumors, which divides efficacy into the subgroups: CR, complete remission; PR, partial remission; SD, no change; PD, progressive disease; CR+PR, overall response rate (13).

Follow-up cases

During follow-up, all patients were contacted via telephone and the last date of follow-up was March 1 2010. A total of 3 cases were lost; thus, the follow-up rate was 93.8%. The follow-up time ranged from 6 to 50 months and the average time was 16 months. The survival time was calculated from the diagnostic date of esophageal carcinoma to the date of mortality or the last date of follow-up.

Antibody

The antibody used in this study was rabbit polyclonal antibody anti-human cyclin A (Thermo Fisher Scientific, Fremont, CA, USA). The final diluted concentration for anti-cyclin A in TBS containing 1% bovine serum albumin (BSA) was 1:100.

Immunohistochemical staining

Sliced sections (4 μm) of paraffin-embedded specimens were prepared on microscope slides pre-coated with saline. Once the paraffin was removed by xylene, the slides were washed in a graded series of ethanol and the sections were placed in Tris-buffered saline (TBS) for 10 min. Sections were then incubated with a blocking solution for 5 min to block endogenous peroxidase activity and placed in TBS. Sections were then placed in 0.01 mM Tris buffer (pH 6.0) and heated at 121°C for 20 min in an autoclave oven. Following this, the sections were incubated with TBS consisting of 1% BSA for 20 min to block non-specific binding of the immunoreagents. Once the cells were washed in TBS, the sections were incubated with 1:100 diluted primary antibodies at 4°C overnight. Following further washing in TBS, an immunoperoxidase staining was performed by a MaxVision antibody complex method using the MaxVision kit (Fujian Maixin Biological Technology, Fujian, China). Finally, the localization of cyclin A was visualized using diaminobenzidine tetrahydrochloride (DAB) and the sections were lightly counterstained in Harris’ hematoxylin solution for microscopic examination.

The immunostained specimens were analyzed by two independent pathologists. At least 10 visual fields were observed and nuclear staining was used to indicate a positive result. A total of 1,000 cells in the tumor and nontumor sections were evaluated at a medium magnification (x200) to determine the proportion of tumor cells and the staining intensity of the nuclei in entire sections. Immunohistochemical expression of cyclin A was determined according to the a+b criteria (14). Category a was defined as the percentage of carcinoma cells with cyclin A expression. The percentage of positively stained tumor cells was determined semi-quantitatively by assessing the whole tumor section, and each sample was assigned to one of the following categories: 0, 0% cyclin A expression; 1, 1–25%; 2, 26–50%; 3, >50%. Category b was defined by the intensity of cyclin A staining. The intensity of immunostaining was defined as 0 (negative), 1 (weak), 2 (moderate) and 3 (strong). The carcinomas were regarded to have a positive response to cyclin A when the total scores of a+b were >3.

Statistical analysis

The Chi-square test was used to examine the difference between the two groups, and the Cox proportional hazards model was used to analyze the association of overall survival in cyclin A positive and negative expression groups. Variables significantly associated with survival in univariate Cox models were included in a multivariate Cox model. The results were quantified by calculating the hazard ratios (HRs) with 95% confidence intervals (CIs). Survival curves were calculated using the Kaplan-Meier method and differences between the curves were determined using the log-rank test.

In all tests, P<0.05 was considered to indicate a statistically significant difference. Statistical calculations were conducted using the SPSS version 16.0 System (SPSS, Chicago, IL, USA).

Results

Expression pattern of cyclin A in normal human esophageal mucosa and ESCC

Cyclin A was expressed mainly in the nuclei of cancer cells and in the basal cells of normal esophageal mucosa (P<0.05; Fig. 1). The positive cyclin A expression rate was 64.6% (31/48) in 48 cases with ESCC, and 20% (2/10) in 10 cases with normal esophageal mucosa. Comparisons between cyclin A expression pattern in ESCC and normal tissues have been revealed in our previous studies (10,11).

Cyclin A staining and clinicopathological factors

The correlations between cyclin A expression and clinicopathological factors of ESCC are summarized in Table I. Statistically, the expression of cyclin A was not significantly associated with the age, gender, tumor size or clinical stage of the patients; however, the expression levels of cyclin A were significantly higher in poorly differentiated ESCC cases compared with well-differentiated ESCC cases (χ2=5.274; P<0.05). This results is consistent with our previous studies in post-surgery ESCC samples (10,11).

Table I.

Correlation between cyclin A expression and clinicopathological factors.

Table I.

Correlation between cyclin A expression and clinicopathological factors.

Clinicopathological factorsPositive (%)
n=31
Negative (%)
n=17
χ2P-value
Age (years)
  ≥6025 (71.4)10 (28.6)
  <606 (46.2)7 (53.8)2.6470.104
Gender
  Male21 (63.6)12 (36.4)
  Female10 (66.7)5 (33.3)0.0410.839
Tumor size (cm)
  <514 (56.0)11 (44.0)
  ≥517 (73.9)6 (26.1)1.6800.195
TNM stage
  I–III12 (60.0)8 (40.0)
  IV19 (67.9)9 (32.1)0.3150.575
Differentiation
  Well/moderate13 (50.0)13 (50.0)
  Poor18 (81.8)4 (18.2)5.2740.022
Cyclin A staining and short-term treatment efficacy

Of the 48 ESCC patients, the number of CR, PR, SD and PD cases were 6, 15, 24 and 3, respectively. The overall response rate (CR+PR) was 43.8% (21/48). According to the expression levels of cyclin A, the response rate was 54.8% (17/31) and 23.5% (4/17) in the positive and negative group, respectively (χ2=4.373; P=0.037; Table II).

Table II.

Correlation between cyclin A expression and short-term treating efficacy.

Table II.

Correlation between cyclin A expression and short-term treating efficacy.

EfficacyPositive
n=31 (%)
Negative
n=17 (%)
χ2P-value
CR5 (16.13)1 (5.88)1.0540.305
PR12 (38.71)3 (17.65)2.2670.132
SD13 (41.94)11 (64.71)0.0240.877
PD1 (3.22)2 (11.76)4.2960.039
CR+PRa17 (54.84)4 (23.53)4.3730.037

a Overall response rate. CR, complete remission; PR, partial remission; SD, no change; PD, progressive disease.

Cyclin A staining and prognosis

Univariate Cox analysis revealed that the clinicopathological stage, degree of cancer cell differentiation and expression of cyclin A were impact factors for prognosis in ESCC patients; while no significant correlation was observed between age, gender and tumor size (Table III). The median survival time for patients with positive cyclin A expression was 17 months. This was significantly shorter compared with that for the patients with negative cyclin A expression, which was 33 months (P=0.016). Kaplan-Meier survival curves demonstrated that the 1- and 3-year survival rates in patients with positive cyclin A expression were significantly lower compared with that of the patients with negative cyclin A expression (54.9 vs. 82.4%, P=0.037; 6.2 vs. 41.2%, P=0.016, respectively) (Fig. 2).

Table III.

Univariate analysis of prognostic impact factors of ESCC patients.

Table III.

Univariate analysis of prognostic impact factors of ESCC patients.

Impact factorNo.Median survival (months)χ2P-value
Age (years)
  ≥603518
  >6013200.0800.778
Gender
  Male3318
  Female15250.0760.783
Tumor size (cm)
  <52520
  ≥523180.1160.733
TNM stage
  I–III2028
  IV2810.53.9800.046
Differentiation
  Well/moderate2626
  Poor22125.8630.015
Cyclin A expression
  Positive3117
  Negative17335.7490.016

[i] ESCC, esophageal squamous cell carcinoma.

Multivariate Cox analysis revealed that the clinicopathological stage, degree of cancer cell differentiation and expression of cyclin A were of statistical significance in ESCC patients. We demonstrated that advanced clinicopathological stage (P=0.000; 95% CI, 0.018–0.309), poor cancer cell differentiation (P=0.008; 95% CI, 0.138–0.739) and positive expression of cyclin A (P=0.002, 95% CI, 0.119–0.611) were independent prognostic factors in patients with ESCC following paclitaxel-based chemoradiotherapy (Table IV).

Table IV.

Multivariate Cox analysis of clinicopathological and biological molecular characteristics in ESCC patients.

Table IV.

Multivariate Cox analysis of clinicopathological and biological molecular characteristics in ESCC patients.

BSEWaldP-valueExp (B)95% CI for Exp (B)
LowerUpper
TNM stage−2.6070.73212.6930.0000.0740.0180.309
Differentiation−1.4140.4277.1330.0080.3200.1380.739
Cyclin A expression−1.3120.4189.8450.0020.2690.1190.611

[i] ESCC, esophageal squamous cell carcinoma; CI, confidence interval.

Discussion

Since the overall survival rate of ESCC patients following surgery alone is poor, multimodality approaches have been developed. However, these are inadequate with regard to improving the treatment efficacy of esophageal cancer by basing treatment on surgical approaches alone. Instead, surgeons should be prepared for a new approach, which comprises biological tumor staging and targeted therapies combined with neoadjuvant chemoradiotherapy. Certain studies suggest that the tumor response to induction chemoradiotherapy aids the identification of patients with good prognosis, regardless of whether surgery is conducted or not (3,15). In these patients, surgery may no longer be recommended as routine treatment (15); therefore, ESCC patients may have a survival benefit if they are responsive to chemoradiotherapy. To predict the response prior to chemoradiotherapy is undoubtedly one of the critical factors to determine the best treatment. Although not all tumors respond to paclitaxel, it is used widely in the treatment of ESCC. A previous study has revealed that the response rate of paclitaxel as a single drug in ESCC patients was only 20–37%, and the overall response rate of paclitaxel in combination with cisplatin was 42.6% (16). The characteristics that distinguish drug-resistant tumors from drug-sensitive tumors are not well defined, and for that reason, predicting the response prior to chemotherapy is a research hotspot. Therefore, several studies have focused on predicting paclitaxel sensitivity in vitro using BRCA1, CDK1 and CDK2 (17,18).

Cyclins, which control key checkpoints of the cell cycle, play a fundamental role in regulating cell growth and survival. In humans, several types of cyclin, including three categories, A-, B- and C-type (C-, D- and E-types), have now been isolated. Cyclins A and B1–2 reach maximum levels in the S and G2 phases. Cyclin A may be activated during the transition from the G1 to the S phase of the cell cycle; therefore, it is suggested to be an important regulator of mitosis (19,20). In this study, we detected the expression of cyclin A in tissue samples from 48 patients with ESCC prior to treatment with paclitaxel/cisplatin. We revealed that the expression of cyclin A was significantly correlated with the degree of cell differentiation and the response to paclitaxel-based chemotherapy. The expression of cyclin A in the poorly differentiated ESCC samples was higher compared with that of the well-differentiated ESCC samples, indicating that cyclin A expression correlates with the degree of ESCC malignancy. This suggests that ESCC samples with positive cyclin A expression were poorly differentiated, and the cell proliferation cycle was more active. Of the 48 cases of ESCC, the response rate of 31 cases with positive cyclin A expression was 54.8%, while the response rate of 17 cases with negative cyclin A expression was 23.5%. This result indicates that ESCC patients with positive cyclin A expression were sensitive to paclitaxel-based chemotherapy and may have a higher response rate.

Paclitaxel binds to microtubules and stabilizes the polymerized structure. Consequently, paclitaxel inhibits microtubule depolymerization, suppressing tumor growth and holding proliferative cells in the G2/M phase (21). Nakayama et al reported that the analysis of cyclin-dependent kinase activity in the clinical setting may be a powerful approach for predicting paclitaxel sensitivity (18). Another study revealed that targeting cyclin B1 sensitized breast cancer cells to taxol, suggesting that specific cyclin B1 targeting is an attractive strategy to be used in combination with conventionally used agents in gynecological cancer therapy (22). Our study demonstrated that cyclin A expression was correlated with chemosensitivity to paclitaxel-based chemotherapy in patients with ESCC. This indicates that the cell cycle is located at the G2/M phase and that when the expression of cyclin A was positive in tumor cells, patients had a higher response to paclitaxel, which is consistent with the cell cycle theory.

Our data also revealed that ESCC patients with positive cyclin A expression had poor prognosis, while the patients with negative cyclin A expression had good prognosis. This result is consistent with previous studies on breast (23), ovarian (24) and laryngeal cancer (25).

Determining whether the tumor is sensitive to paclitaxel prior to treatment is one of the most efficient ways of improving response. Our study revealed that ESCC patients overexpressing cyclin A prior to treatment may be more sensitive to paclitaxel-based chemotherapy. It would be beneficial to extend this preliminary result into a larger sample size study in order to formalize it as a standard procedure for the clinical treatment of ESCC. Our study indicates the importance of applying ‘personalized cancer medicine’ in the clinical treatment of cancer, which is based on gene expression profile, to guide the antitumor drug selection.

Acknowledgements

This study was supported by the 333 Plan Foundation (Grant No. 2009-24) of Jiangsu Province, China, the Six Talents Peak Foundation (Grant No. 2011-WS-023) of Jiangsu, China, and the Key Medical Talent Foundation (Grant No. RC2011212) of Jiangsu, China. The authors are grateful to Dr Jianming Zhang for his assistance with the manuscript preparation.

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October 2012
Volume 4 Issue 4

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Spandidos Publications style
Huang JX, Shen SL, Lin M, Xiao W, Chen WC, Lin MS, Yu H, Chen P and Qian RY: Cyclin A overexpression is associated with chemosensitivity to paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma. Oncol Lett 4: 607-611, 2012
APA
Huang, J., Shen, S., Lin, M., Xiao, W., Chen, W., Lin, M. ... Qian, R. (2012). Cyclin A overexpression is associated with chemosensitivity to paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma. Oncology Letters, 4, 607-611. https://doi.org/10.3892/ol.2012.814
MLA
Huang, J., Shen, S., Lin, M., Xiao, W., Chen, W., Lin, M., Yu, H., Chen, P., Qian, R."Cyclin A overexpression is associated with chemosensitivity to paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma". Oncology Letters 4.4 (2012): 607-611.
Chicago
Huang, J., Shen, S., Lin, M., Xiao, W., Chen, W., Lin, M., Yu, H., Chen, P., Qian, R."Cyclin A overexpression is associated with chemosensitivity to paclitaxel‑based chemotherapy in patients with esophageal squamous cell carcinoma". Oncology Letters 4, no. 4 (2012): 607-611. https://doi.org/10.3892/ol.2012.814