Ultrasound microbubble contrast agent‑mediated suicide gene transfection in the treatment of hepatic cancer

Tang,Qing; He,Xiaohong; Liao,Haixing; He,Liantu; Wang,Ying; Zhou,Dazhi; Ye,Sumin; Chen,Qilu

doi:10.3892/ol.2012.845

November 2012 Volume 4 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

November 2012 Volume 4 Issue 5

Full Size Image

Article

Ultrasound microbubble contrast agent‑mediated suicide gene transfection in the treatment of hepatic cancer

Authors:
- Qing Tang
- Xiaohong He
- Haixing Liao
- Liantu He
- Ying Wang
- Dazhi Zhou
- Sumin Ye
- Qilu Chen
View Affiliations / Copyright

Affiliations: Department of Sonography, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510120, P.R. China, Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
Pages: 970-972
|
Published online on: August 6, 2012

https://doi.org/10.3892/ol.2012.845
Expand metrics +

Abstract

The aim of this study was to investigate the role of ultrasound microbubble contrast agent‑mediated suicide gene transfection in the treatment of hepatic cancer. We intratumorally injected KDR-TK, AFP-TK and microbubble contrast agent into nude mice prior to ultrasound treatment and administration of prodrugs (GCV and 5-FC). The tumor volume, tumor inhibition rate, survival time and apoptosis of tumor cells was determined. The sizes of subcutaneous hepatic cancers in mice receiving treatment were comparable to those in the control group, and the survival time was similar between the two groups (P>0.05). However, the tumor inhibition rate and the number of apoptotic cells in the treatment group was markedly higher compared with that in the control group (P<0.05). Evident tumor necrosis was absent in both groups, except at the needle tract. Ultrasound therapy following injection of suicide genes and microbubble contrast agents is able to inhibit cancer growth in vivo. This may be attributed to the induction of cancer cell apoptosis.

View Figures

View References

1.	B DegrèveE De ClercqJ BalzariniBystander effect of purine nucleoside analogues in HSV-tk suicide gene therapy is superior to that of pyrimidine nucleoside analoguesGene Ther6162170199910435100
2.	VG ZarnitsynMR PrausnitzPhysical parameters influencing optimization of ultrasound-mediated DNA transfectionUltrasound Med Biol30527538200410.1016/j.ultrasmedbio.2004.01.00815121255
3.	PA DijkmansLJ JuffermansRJ MustersMicrobubbles and ultrasound: from diagnosis to therapyEur J Echocardiogr5245256200410.1016/j.euje.2004.02.00115219539
4.	K FerraraR PollardM BordenUltrasound microbubble contrast agents: fundamentals and application to gene and drug deliveryAnnu Rev Biomed Eng9415447200710.1146/annurev.bioeng.8.061505.09585217651012
5.	A AoiY WatanabeS MoriHerpes simplex virus thymidine kinase-mediated suicide gene therapy using nano/microbubbles and ultrasoundUltrasound Med Biol34425434200810.1016/j.ultrasmedbio.2007.09.00418096302
6.	QL LuHD LiangT PartridgeMJ BlomleyMicrobubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damageGene Ther10396405200310.1038/sj.gt.330191312601394
7.	Q TangHX XuMD lvF NieH YangY WangUltrasound contrast agent enhancing changes of murine hepatocellular carcinoma microvascular permeability caused by ultrasound irradiationChin J Ultra Med224114132006
8.	N SheikovN McDannoldS SharmaK HynynenEffect of focused ultrasound applied with an ultrasound contrast agent on the tight junctional integrity of the brain microvascular endotheliumUltrasound Med Biol3410931104200810.1016/j.ultrasmedbio.2007.12.015
9.	P SchratzbergerJG KraininG SchratzbgerTranscutaneous ultrasound augments naked DNA transfection of skeletal muscleMol Ther6576583200210.1016/S1525-0016(02)90715-X12409255
10.	XH LiP ZhouLH WangThe targeted gene (KDRP-CD/TK) therapy of breast cancer mediated by SonoVue and ultrasound irradiation in vitroUltrasonics52186191201210.1016/j.ultras.2011.08.00221906771

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Ultrasound microbubble contrast agent‑mediated suicide gene transfection in the treatment of hepatic cancer

This article is mentioned in:

Abstract

Figure 1

Related Articles