Expression of deleted in liver cancer 2 in colorectal cancer and its correlation with clinicopathological parameters

Gao,Kai; Li,Xiaorong; Hu,Gui; Yang,Kaiyan; Tian,Buning; Zhang,Yi

doi:10.3892/ol.2012.854

November 2012 Volume 4 Issue 5

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November 2012 Volume 4 Issue 5

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Article

Expression of deleted in liver cancer 2 in colorectal cancer and its correlation with clinicopathological parameters

Authors:
- Kai Gao
- Xiaorong Li
- Gui Hu
- Kaiyan Yang
- Buning Tian
- Yi Zhang
View Affiliations / Copyright

Affiliations: Department of General Surgery, The Third Affiliated Hospital of Central South University, Central South University, Changsha, Hunan 410013, P.R. China
Pages: 988-992
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Published online on: August 6, 2012

https://doi.org/10.3892/ol.2012.854
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Abstract

Deleted in liver cancer 2 (DLC2) has been identified as a tumor suppressor gene. DLC2 is closely related to deleted in liver cancer 1 (DLC1) and is located at chromosome 13q12.3. The expression of DLC2 mRNA has been found in a wide range of cancers, including colorectal cancer (CRC). However, there are no available data on the expression status of DLC2 in Chinese patients with CRC and its correlation with clinicopathological parameters. The aim of this study was to investigate the expression levels of DLC2 mRNA and protein in Chinese patients with CRC and the correlation between DLC2 expression and clinicopathological parameters. To this end, real-time PCR, western blotting and immunohistochemistry were employed to detect DLC2 mRNA and protein expression in CRC and pericarcinomatous intestinal tissue (PCIT) specimens, which were obtained from 102 Chinese CRC patients who underwent surgical resection between October 2010 and February 2012. We also analyzed the correlations between DLC2 expression and the clinicopathological parameters of CRC patients. Our results showed that CRC tissues had significantly lower levels of DLC2 mRNA compared with PCITs (P<0.05); however, the protein expression levels were not significantly different between CRCs and PCITs. The expression levels of DLC2 mRNA and protein were significantly correlated with lymph node metastasis and tumor TNM stage. Additionally, DLC2 mRNA expression levels were also correlated with tumor histopathological degree (P<0.05). Collectively, our results suggest that the downregulated expression of DLC2 participates in CRC carcinogenesis, invasion and lymph node metastasis. Furthermore, our results imply that DLC2 is be a potential prognostic marker for CRC patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of deleted in liver cancer 2 in colorectal cancer and its correlation with clinicopathological parameters

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