DBC1 does not function as a negative regulator of SIRT1 in liver cancer

Bae,Hyun  Jin; Chang,Young  Gyoon; Noh,Ji  Heon; Kim,Jeong  Kyu; Eun,Jung  Woo; Jung,Kwang  Hwa; Kim,Min  Gyu; Shen,Qingyu; Ahn,Young  Min; Kwon,So  Hee; Park,Won  Sang; Lee,Jung  Young; Nam,Suk  Woo

doi:10.3892/ol.2012.875

DBC1 does not function as a negative regulator of SIRT1 in liver cancer

Authors:
- Hyun Jin Bae
- Young Gyoon Chang
- Ji Heon Noh
- Jeong Kyu Kim
- Jung Woo Eun
- Kwang Hwa Jung
- Min Gyu Kim
- Qingyu Shen
- Young Min Ahn
- So Hee Kwon
- Won Sang Park
- Jung Young Lee
- Suk Woo Nam
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Affiliations: Laboratory of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea, Functional RNomics Research Center, The Catholic University of Korea, Seoul, Republic of Korea, Department of Kidney System, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea, College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Republic of Korea
Published online on: August 23, 2012 https://doi.org/10.3892/ol.2012.875
Pages: 873-877

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Abstract

The putative tumor suppressor, DBC1 (deleted in breast cancer-1), was recently found to negatively regulate SIRT1 in vitro and in vivo, but the mechanism whereby DBC1 regulates SIRT1 in liver cancer remains to be elucidated. In this study, it was found that although the expression of DBC1 and SIRT1 was not aberrantly regulated in a large cohort of human hepatocellular carcinoma (HCC) patients, these proteins were highly overexpressed in a subset of HCC tissues compared with surrounding non-cancer tissues. In liver cancer, DBC1 and SIRT1 were found to be positively correlated. Inactivation of DBC1 or SIRT1 reduced SNU-182 (a liver cancer cell line) proliferation as determined by MTT viability assays. Notably, although DBC1 functions as a negative regulator of SIRT1 in A549 lung cancer cells since it suppresses the deacetylase activity of the p53 protein, it did not affect the p53 deacetylase activity of SIRT1 in SNU-182 cells. Taken together, we conclude that DBC1 is associated with SIRT1 in HCC, but that it does not inhibit SIRT1.

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