Detection of the Epstein-Barr virus in blood and bone marrow mononuclear cells of patients with aggressive B-cell non‑Hodgkin's lymphoma is not associated with prognosis

Marques,Herlander; Catarino,Raquel; Domingues,Nelson; Barros,Eliane; Portela,Catarina; Almeida,Maria Inês; Costa,Sandra; Reis,Rui Manuel; Medeiros,Rui; Longatto-Filho,Adhemar

doi:10.3892/ol.2012.913

December 2012 Volume 4 Issue 6

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December 2012 Volume 4 Issue 6

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Article

Detection of the Epstein-Barr virus in blood and bone marrow mononuclear cells of patients with aggressive B-cell non‑Hodgkin's lymphoma is not associated with prognosis

Authors:
- Herlander Marques
- Raquel Catarino
- Nelson Domingues
- Eliane Barros
- Catarina Portela
- Maria Inês Almeida
- Sandra Costa
- Rui Manuel Reis
- Rui Medeiros
- Adhemar Longatto-Filho
View Affiliations / Copyright

Affiliations: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal, Molecular Oncology Group and Virology Laboratory, Portuguese Institute of Oncology, Porto, Portugal, Oncology‑Hematology Department, Institute of Oncology, Porto, Portugal, Department of Oncology, Hospital of Braga, Braga, Portugal
Pages: 1285-1289
|
Published online on: September 12, 2012

https://doi.org/10.3892/ol.2012.913
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Abstract

The Epstein-Barr virus (EBV) is associated with a large spectrum of lymphoproliferative diseases. Traditional methods of EBV detection include the immunohistochemical identification of viral proteins and DNA probes to the viral genome in tumoral tissue. The present study explored the detection of the EBV genome, using the BALF5 gene, in the bone marrow or blood mononuclear cells of patients with diffuse large B-cell lymphomas (DLBCL) and related its presence to the clinical variables and risk factors. The results show that EBV detection in 21.5% of patients is not associated with age, gender, staging, B symptoms, international prognostic index scores or any analytical parameters, including lactate dehydrogenase (LDH) or β-2 microglobulin (B2M). The majority of patients were treated with R-CHOP‑like (rituximab, cyclophosphamide, doxorubicin, vincristine and prednisolone or an equivalent combination) and some with CHOP-like chemotherapy. Response rates [complete response (CR) + partial response (PR)] were not significantly different between EBV-negative and -positive cases, with 93.2 and 88.9%, respectively. The survival rate was also similar in the two groups, with 5-year overall survival (OS) rates of 64.3 and 76.7%, respectively. However, when analyzing the treatment groups separately there was a trend in EBV-positive patients for a worse prognosis in patients treated with CHOP-like regimens that was not identified in patients treated with R-CHOP-like regimens. We conclude that EBV detection in the bone marrow and blood mononuclear cells of DLBC patients has the same frequency of EBV detection on tumoral lymphoma tissue but is not associated with the risk factors, response rate and survival in patients treated mainly with immunochemotherapy plus rituximab. These results also suggest that the addition of rituximab to chemotherapy improves the prognosis associated with EBV detection in DLBCL.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of the Epstein-Barr virus in blood and bone marrow mononuclear cells of patients with aggressive B-cell non‑Hodgkin's lymphoma is not associated with prognosis

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