GRP78 and GAL3, differentially regulated by lymph node homogenates, as potential biomarkers for lymph node metastasis in mouse hepatocellular carcinoma cells

Zhu,Wenjun; Owusu,Lawrence; Zang,Shizhu; Zhang,Yunjuan; Xin,Yi; Yan,Chao

doi:10.3892/ol.2012.915

December 2012 Volume 4 Issue 6

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December 2012 Volume 4 Issue 6

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Article

GRP78 and GAL3, differentially regulated by lymph node homogenates, as potential biomarkers for lymph node metastasis in mouse hepatocellular carcinoma cells

Authors:
- Wenjun Zhu
- Lawrence Owusu
- Shizhu Zang
- Yunjuan Zhang
- Yi Xin
- Chao Yan
View Affiliations / Copyright

Affiliations: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning116044, P.R. China, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P.R. China
Pages: 1374-1378
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Published online on: September 13, 2012

https://doi.org/10.3892/ol.2012.915
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Abstract

In order to systematically evaluate the influence of lymph nodes (LNs) in lymph node metastases (LNM) of hepatocellular carcinoma (HCC), we set up a new in vitro model in which Hca‑F and Hca‑P cells were cultured in medium containing lymph node homogenates (LNHs). Differential protein expression was measured by two‑dimensional gel electrophoresis (2‑DE) combined with matrix‑assisted laser desorption/ionization time‑of‑flight/time‑of‑flight mass spectrometry (MALDI TOF/TOF MS). Results from protein identification revealed two metastatic correlative proteins, 78-kDa glucose‑regulated protein (GRP78) and galectin-3 (GAL3). Western blotting confirmed that GRP78, a protein positively correlated with metastasis, increased 2.4‑fold in Hca‑F cells but decreased to almost a half in Hca‑P cells (P<0.05). However, GAL3, a protein negatively correlated with metastasis, was decreased by a half in Hca‑F cells but slightly increased non‑significantly in Hca‑P cells. Thus, our results reveal that some components of LNHs may facilitate a permissive environment for cancer cells with high metastasis potential to eventually metastasize. GRP78 and GAL3 may serve as potential biomarkers for the diagnosis of LNM in HCC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

GRP78 and GAL3, differentially regulated by lymph node homogenates, as potential biomarkers for lymph node metastasis in mouse hepatocellular carcinoma cells

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