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Article

Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines

  • Authors:
    • Gui-Qin Ling
    • Dong-Bo Chen
    • Bao-Qing Wang
    • Lan-Sheng Zhang
  • View Affiliations / Copyright

    Affiliations: The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R.China
  • Pages: 1264-1268
    |
    Published online on: September 13, 2012
       https://doi.org/10.3892/ol.2012.916
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Abstract

Previous studies have demonstrated that pluripotency-associated transcription factors, such as Oct3/4, Nanog and Sox2, play a crucial role in the development and malignant progression of various types of tumors. Breast cancer is the most frequent cancer among females, being a heterogeneous disease, with distinct morphologies, metastatic behavior and therapeutic responses. The expression of Oct3/4, Nanog and Sox2 in 3 human breast cancer cell lines, MCF7, T-47D and MDA-MB‑231, was determined. The expression of Oct3/4, Nanog and Sox2 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR) and protein expression was detected by immunocytohistochemistry. RT-PCR revealed that all three human breast cancer cell lines tested expressed evident Oct3/4, Nanog and Sox-2 mRNA at various levels. Higher levels of Oct3/4 were identified in MCF7 and MDA-MB-231 cells compared with T-47D cells. Higher levels of Nanog were observed in MCF7 and T-47D cells compared with MDA-MB-231 cells and the highest expression of Sox-2 was detected in MCF7 cells. The nuclear localization of Oct3/4, Nanog and Sox-2 was confirmed by immunostaining. Oct3/4, Nanog and Sox2 were expressed in human breast cancer cell lines. Further studies are required to characterize the role of Oct3/4, Nanog and Sox2 in human breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ling G, Chen D, Wang B and Zhang L: Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines. Oncol Lett 4: 1264-1268, 2012.
APA
Ling, G., Chen, D., Wang, B., & Zhang, L. (2012). Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines. Oncology Letters, 4, 1264-1268. https://doi.org/10.3892/ol.2012.916
MLA
Ling, G., Chen, D., Wang, B., Zhang, L."Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines". Oncology Letters 4.6 (2012): 1264-1268.
Chicago
Ling, G., Chen, D., Wang, B., Zhang, L."Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines". Oncology Letters 4, no. 6 (2012): 1264-1268. https://doi.org/10.3892/ol.2012.916
Copy and paste a formatted citation
x
Spandidos Publications style
Ling G, Chen D, Wang B and Zhang L: Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines. Oncol Lett 4: 1264-1268, 2012.
APA
Ling, G., Chen, D., Wang, B., & Zhang, L. (2012). Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines. Oncology Letters, 4, 1264-1268. https://doi.org/10.3892/ol.2012.916
MLA
Ling, G., Chen, D., Wang, B., Zhang, L."Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines". Oncology Letters 4.6 (2012): 1264-1268.
Chicago
Ling, G., Chen, D., Wang, B., Zhang, L."Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines". Oncology Letters 4, no. 6 (2012): 1264-1268. https://doi.org/10.3892/ol.2012.916
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