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Article

Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression

Corrigendum in: /10.3892/ol.2020.11421
  • Authors:
    • Liangsheng Fan
    • Hongyan Wang
    • Xi Xia
    • Yumei Rao
    • Xiangyi Ma
    • Ding Ma
    • Peng Wu
    • Gang Chen
  • View Affiliations / Copyright

    Affiliations: Cancer Biology Medical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Obstetrics and Gynecology, South Mountain Hospital of Guangdong Medical College, Shenzhen, Guangdong 518000, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1225-1233
    |
    Published online on: September 21, 2012
       https://doi.org/10.3892/ol.2012.934
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Abstract

E‑cadherin is a key cell‑to‑cell adhesion molecule associated with the invasion and metastasis of tumor cells; however, the molecular mechanisms are not entirely understood. In this study, we investigated whether downregulation of E‑cadherin by E‑cadherin‑specific small intefering RNA (siRNA) was able to promote malignant phenotypes of prostate cancer cells through upregulating the metastasis‑associated gene 1 (MTA1) in vitro. The expression levels of E‑cadherin in human paired prostate adenocarcinoma cell lines, PC‑3M‑2B4 (2B4) and PC‑3M‑1E8 (1E8), were investigated using western blot analysis. The alteration of malignant phenotypes associated with decreasing E‑cadherin expression were assessed in 2B4 cells using wound‑healing assays, solid‑phase adhesion assays, invasion assays and cytoskeletal staining. The expression of E‑cadherin and MTA1 in normal, localized and metastatic prostate cancer cells was analyzed using immunohistochemistry. Downregulation of E‑cadherin using an RNA interference approach led to the upregulation of MTA1 expression, decreased tumor cell adhesion ability as well as enhanced cell mobility, invasion and cellular polarity compared with the controls (P<0.05). E‑cadherin regulated MTA1 in a time‑dependent manner. The correlation between E‑cadherin and MTA1 was inversed in the prostate cancer group (P<0.05; rs=‑0.434). The data suggest that E‑cadherin plays an important role in prostate cancer metastasis, which is likely to be due to the regulation of MTA1 expression. E‑cadherin may combine with MTA1 and alter the malignant phenotype of prostate cancer cells. A combined testing strategy for detecting MTA1 and E‑cadherin may be sufficient for selecting high‑risk patients with metastasis. Therefore, E‑cadherin and MTA1 may be potential powerful factors for the treatment of various types of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fan L, Wang H, Xia X, Rao Y, Ma X, Ma D, Wu P and Chen G: Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421. Oncol Lett 4: 1225-1233, 2012.
APA
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D. ... Chen, G. (2012). Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421. Oncology Letters, 4, 1225-1233. https://doi.org/10.3892/ol.2012.934
MLA
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D., Wu, P., Chen, G."Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421". Oncology Letters 4.6 (2012): 1225-1233.
Chicago
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D., Wu, P., Chen, G."Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421". Oncology Letters 4, no. 6 (2012): 1225-1233. https://doi.org/10.3892/ol.2012.934
Copy and paste a formatted citation
x
Spandidos Publications style
Fan L, Wang H, Xia X, Rao Y, Ma X, Ma D, Wu P and Chen G: Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421. Oncol Lett 4: 1225-1233, 2012.
APA
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D. ... Chen, G. (2012). Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421. Oncology Letters, 4, 1225-1233. https://doi.org/10.3892/ol.2012.934
MLA
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D., Wu, P., Chen, G."Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421". Oncology Letters 4.6 (2012): 1225-1233.
Chicago
Fan, L., Wang, H., Xia, X., Rao, Y., Ma, X., Ma, D., Wu, P., Chen, G."Loss of E‑cadherin promotes prostate cancer metastasis via upregulation of metastasis‑associated gene 1 expression Corrigendum in /10.3892/ol.2020.11421". Oncology Letters 4, no. 6 (2012): 1225-1233. https://doi.org/10.3892/ol.2012.934
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