Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December 2012 Volume 4 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 2012 Volume 4 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study

  • Authors:
    • Kuo-Shyang Jeng
    • I-Shyan Sheen
    • Wen-Juei Jeng
    • Ming‑Che  Yu
    • Hsin-Hua Tsai
    • Fang-Yu Chang
    • Jui-Chih Su
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Far Eastern Memorial Hospital, Taipei, Taiwan, R.O.C., Department of Hepatogastroenterology, Chang Gung Memorial Hospital, Taipei, Taiwan, R.O.C., Department of Medical Research, Far Eastern Memorial Hospital, Taipei, Taiwan, R.O.C.
  • Pages: 1158-1162
    |
    Published online on: September 24, 2012
       https://doi.org/10.3892/ol.2012.935
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hepatocellular carcinoma (HCC), a worldwide malignancy, is prevalent in Asian countries. For individuals with unresectable HCC, the effect of chemotherapy or the present target therapy is limited. There is an urgent need to find innovative new therapies. It is believed that sonic hedgehog (Shh) pathway activation may be essential for hepatocarcinogenesis. In the present study, we conducted an in vivo animal study using an Shh pathway inhibitor to elucidate the effect of treatment upon mice with HCC. Eighty C57BL/6 mice were divided into 4 groups (groups A, B, C and D, with group A serving as a control; n=20 for each). We injected mouse hepatoma Mistheton Lectin-1 cells (5xl06 cells/20 µl) into the left liver of each mouse in groups B, C and D. In the second week, we analyzed each mouse to assess the tumor growth status. Following the tumor injection, group B did not receive any additional intraperitoneal (i.p.) injection, group C received cyclopamine 10 mg/kg/day i.p. and group D received cyclopamine 30 mg/kg/day i.p. every day for 10 days. After an interval of 4 weeks, harvesting and analysis of the liver was performed for each mouse. Tumor size measurement and real‑time PCR of Shh pathway factors (Shh, Ptch-1, Gli-1 and Smoh) for livers of group A and tumors of group B, C and D were undertaken. The decrease in the tumor size of group D was found to be statistically significant (P=0.047) when compared with groups B or C. The decrease of Shh mRNA of both groups C and D had borderline significance when compared with group B. However, Gli-1 mRNA of group D has statistically significant difference (P=0.044) when compared with group A, B or C. Inhibition of the Shh pathway significantly decreases the size and Gli-1 mRNA expression of the tumor. The Shh pathway may be an effective treatment target for HCC in the future.
View Figures

Figure 1

View References

1. 

FX BoschJ RibesJ BorràsEpidemiology of primary liver cancerSemin Liver Dis19271285199910.1055/s-2007-100711710518307

2. 

DM ParkinF BrayJ FerlayP PisaniEstimating the world cancer burden: Globocan 2000Int J Cancer94153156200110.1002/ijc.144011668491

3. 

AP VenookC PapandreouJ FuruseLL de GuevaraThe incidence and epidemiology of hepatocellular carcinoma: a global and regional perspectiveOncologist15513201010.1634/theoncologist.2010-S4-0521115576

4. 

MF ChenTL HwangLB JengPostoperative recurrence of hepatocellular carcinoma: 205 consecutive patients who underwent hepatic resection in 15 yearsArch Surg12973874219947517662

5. 

KS JengIS SheenYC TsaiDoes the presence of circulating hepatocellular carcinoma cells indicate a risk of recurrence after resection?Am J Gastroenterol9915031509200410.1111/j.1572-0241.2004.30227.x15307868

6. 

AE BaleKP YuThe hedghog pathway and basal cell carcinomasHum Mol Genet10757762200110.1093/hmg/10.7.75711257109

7. 

O LupiCorrelations between the sonic hedgehog pathway and basal cell carcinomaInt J Dermatol4611131117200710.1111/j.1365-4632.2007.03391.x17988327

8. 

SS KarhadkarGS BovaN AbdallahHedgehog signaling in prostate regeneration, neoplasia and metastasisNature431707712200410.1038/nature0296215361885

9. 

DN WarkinsCD PeacockHedgehog signalling in foregut malignancyBiochem Pharmacol6810551060200410.1016/j.bcp.2004.04.02515313401

10. 

X MaK ChenS HuangFrequent activation of the hedgehog pathway in advanced gastric adenocarcinomasCarcinogenesis2616981705200510.1093/carcin/bgi13015905200

11. 

X MaT ShengY ZhangHedgehog signaling is activated in subsets of esophageal cancersInt J Cancer118139148200610.1002/ijc.2129516003737

12. 

NS ChariTJ McDonnellThe sonic hedgehog signaling network in development and neoplasiaAdv Anat Pathol14344352200710.1097/PAP.0b013e3180ca8a1d17717435

13. 

SP ThayerMP di MaglianoPW HeiserHedgehog is an early and late mediator of pancreatic cancer tumorigenesisNature425851856200310.1038/nature0200914520413

14. 

S HuangJ HeX ZhangActivation of the hedgehog pathway in human hepatocellular carcinomasCarcinogenesis2713341340200610.1093/carcin/bgi37816501253

15. 

JK SicklickYX LiA JayaramanDysregulation of the hedgehog pathway in human hepatocarcinogenesisCarcinogenesis27748757200610.1093/carcin/bgi29216339184

16. 

MA PatilJ ZhangC HoST CheungHedgehog signaling in human hepatocellular carcinomaCancer Biol Ther5111117200610.4161/cbt.5.1.237916397407

17. 

L CheJ RenYH YuanExpression of genes related to sonic hedgehog signaling in human hepatocellular carcinomasBeijing Da Xue Xue Bao406166232008(In Chinese).

18. 

X FuQ WangX ChenExpression patterns and polymorphisms of PTCH in Chinese hepatocellular carcinoma patientsExp Mol Pathol84195199200810.1016/j.yexmp.2008.04.00218538319

19. 

XL ChenQY ChengMR SheExpression of sonic hedgehog signaling components in hepatocellular carcinoma and cyclopamine-induced apoptosis through Bcl-2 downregulation in vitroArch Med Res41315323201010.1016/j.arcmed.2010.06.00320851287

20. 

WT ChengK XuDY TianZG ZhangLJ LiuY ChenRole of Hedgehog signaling pathway in proliferation and invasiveness of hepatocellular carcinoma cellsInt J Oncol34829836200919212688

21. 

M TadaF KanaiY TanakaDown-regulation of hedgehog-interacting protein through genetic and epigenetic alterations in human hepatocellular carcinomaClin Cancer Res1437683776200810.1158/1078-0432.CCR-07-118118559595

22. 

J TaipalePA BeachyThe Hedgehog and Wnt signaling pathways in cancerNature411349354200110.1038/3507721911357142

23. 

F TianJ MysliwietzJ EllwartEffects of the hedgehog pathway inhibitor GDC-0449 on lung cancer cell lines are mediated by side populationsClin Exp Med122530201210.1007/s10238-011-0135-821519961

24. 

FC KelleherHedgehog signaling and therapeutics in pancreatic cancerCarcinogenesis32445451201110.1093/carcin/bgq28021186299

25. 

Y KimJW YoonX XiaoNM DeanBP MoniaEG MarcussonSelective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cellsCancer Res6735833593200710.1158/0008-5472.CAN-06-304017440069

26. 

M EichenmüllerI GrunerB HaglBlocking the hedgehog pathway inhibits hepatoblastoma growthHepatology49482490200919177589

27. 

C AdolpheR HetheringtonT EllisB WainwrightPatched1 functions as a gatekeeper by promoting cell cycle progressionCancer Res6620812088200610.1158/0008-5472.CAN-05-214616489008

28. 

YJ KimJY ParkSW ParkThe sonic hedgehog pathway as a treatment target for extrahepatic biliary tract cancerMol Med Report51216201221946948

29. 

MH TaiCC ChangM KiupelOct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesisCarcinogenesis26495502200515513931

30. 

CT JordanSearching for leukemia stem cells - not yet the end of the road?Cancer Cell10253254200610.1016/j.ccr.2006.09.01017045202

31. 

M ShackletonE QuintanaER FearonSJ MorrisonHeterogeneity in cancer: cancer stem cells versus clonal evolutionCell138822829200910.1016/j.cell.2009.08.01719737509

32. 

JM BaileyAM MohrMA HollingsworthSonic hedgehog paracrine signaling regulates metastasis and lymphangiogenesis in pancreatic cancerOncogene2835133525200910.1038/onc.2009.22019633682

33. 

M NakamuraM KuboS NagaiNew therapeutic strategy for cancer targeting the hedgehog signaling pathwayGan To Kagaku Ryoho34191419162007(In Japanese).

34. 

RLH BigelowNS ChariAB UndenTranscriptional regulation of bcl-2 mediated by the sonic hedgehog signaling pathway through gli-1J Biol Chem279119712052004

35. 

P SanchezAM HernándezB SteccaInhibition of prostate cancer proliferation by interference with sonic hedgehog-Gli-1 signalingProc Natl Acad Sci USA1011256112566200410.1073/pnas.040495610115314219

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jeng K, Sheen I, Jeng W, Yu MC, Tsai H, Chang F and Su J: Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study. Oncol Lett 4: 1158-1162, 2012.
APA
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., & Su, J. (2012). Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study. Oncology Letters, 4, 1158-1162. https://doi.org/10.3892/ol.2012.935
MLA
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., Su, J."Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study". Oncology Letters 4.6 (2012): 1158-1162.
Chicago
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., Su, J."Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study". Oncology Letters 4, no. 6 (2012): 1158-1162. https://doi.org/10.3892/ol.2012.935
Copy and paste a formatted citation
x
Spandidos Publications style
Jeng K, Sheen I, Jeng W, Yu MC, Tsai H, Chang F and Su J: Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study. Oncol Lett 4: 1158-1162, 2012.
APA
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., & Su, J. (2012). Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study. Oncology Letters, 4, 1158-1162. https://doi.org/10.3892/ol.2012.935
MLA
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., Su, J."Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study". Oncology Letters 4.6 (2012): 1158-1162.
Chicago
Jeng, K., Sheen, I., Jeng, W., Yu, M., Tsai, H., Chang, F., Su, J."Blockade of the sonic hedgehog pathway effectively inhibits the growth of hepatoma in mice: An in vivo study". Oncology Letters 4, no. 6 (2012): 1158-1162. https://doi.org/10.3892/ol.2012.935
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team