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Article

Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma

  • Authors:
    • Xiu-Cheng Pan
    • Li Li
    • Juan-Juan Mao
    • Wei Yao
    • Jun-Nian Zheng
    • Mei Liu
    • Juan-Juan Fu
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China, Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China
  • Pages: 90-96
    |
    Published online on: October 12, 2012
       https://doi.org/10.3892/ol.2012.966
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Abstract

Cancer immunotherapies are designed to elicit T-cell responses that inhibit tumor growth. Previous studies have demonstrated that interleukin 21 (IL-21) is a promising cytokine for cancer immunotherapy due to its ability to induce the immunity of T cells and natural killer cells, whereas blockade of the interaction of programmed death receptor-1 (PD-1) with its ligand (PD-L1) reduces peripheral tolerance. In the current study, we investigated IL-21 alone and in combination with soluble PD-1 (sPD-1) for the treatment of experimental H22 murine hepatocarcinoma. The naked plasmids pmIL-21 and/or psPD-1 were used for local gene transfer by injection. In these assays, sPD-1 combined with IL-21 was found to significantly inhibit the growth of the tumors in mice. Combined treatment with IL-21 and sPD-1 enhanced the antitumor immune response compared with that induced by IL-21 alone. Combined treatment was found to increase CTL cytotoxicity, increase the number of CTLs and NK cells in splenocytes, upregulate the cytokines IFN-γ and IL-2 and downregulate IL-10. Thus, immunotherapy with IL-21 in combination with sPD-1 was found to induce a more efficacious antitumor immune response, which may have potential clinical implications.
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Copy and paste a formatted citation
Spandidos Publications style
Pan X, Li L, Mao J, Yao W, Zheng J, Liu M and Fu J: Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma. Oncol Lett 5: 90-96, 2013.
APA
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., & Fu, J. (2013). Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma. Oncology Letters, 5, 90-96. https://doi.org/10.3892/ol.2012.966
MLA
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., Fu, J."Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma". Oncology Letters 5.1 (2013): 90-96.
Chicago
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., Fu, J."Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma". Oncology Letters 5, no. 1 (2013): 90-96. https://doi.org/10.3892/ol.2012.966
Copy and paste a formatted citation
x
Spandidos Publications style
Pan X, Li L, Mao J, Yao W, Zheng J, Liu M and Fu J: Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma. Oncol Lett 5: 90-96, 2013.
APA
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., & Fu, J. (2013). Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma. Oncology Letters, 5, 90-96. https://doi.org/10.3892/ol.2012.966
MLA
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., Fu, J."Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma". Oncology Letters 5.1 (2013): 90-96.
Chicago
Pan, X., Li, L., Mao, J., Yao, W., Zheng, J., Liu, M., Fu, J."Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma". Oncology Letters 5, no. 1 (2013): 90-96. https://doi.org/10.3892/ol.2012.966
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