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Article

Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies

  • Authors:
    • Gretel Mendoza
    • Amelia Portillo
    • Jorge Olmos‑Soto
  • View Affiliations / Copyright

    Affiliations: Molecular Microbiology Laboratory, Department of Marine Biotechnology, Center for Scientific Research and Education (CICESE), Ensenada, Baja California 22860, Mexico, Science Faculty, Autonomous University of Baja California (UABC), Ensenada, Baja California 22860, Mexico
  • Pages: 295-298
    |
    Published online on: October 22, 2012
       https://doi.org/10.3892/ol.2012.984
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Abstract

her-2 gene amplification and its overexpression in breast cancer cells is directly associated with aggressive clinical behavior. The her-2 gene and its Her-2 protein have been utilized for disease diagnosis and as a predictive marker for treatment response to the antibody herceptin. Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common FDA-approved methodologies involving gene and protein quantification, respectively. False positive or negative her-2/Her-2 patient results may result in inappropriate treatment administration. To support accurate quantification and interpretation of results, in this study we have standardized qPCR analysis using previously identified IHC samples, obtaining very significant and clinically useful results.
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Copy and paste a formatted citation
Spandidos Publications style
Mendoza G, Portillo A and Olmos‑Soto J: Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies. Oncol Lett 5: 295-298, 2013.
APA
Mendoza, G., Portillo, A., & Olmos‑Soto, J. (2013). Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies. Oncology Letters, 5, 295-298. https://doi.org/10.3892/ol.2012.984
MLA
Mendoza, G., Portillo, A., Olmos‑Soto, J."Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies". Oncology Letters 5.1 (2013): 295-298.
Chicago
Mendoza, G., Portillo, A., Olmos‑Soto, J."Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies". Oncology Letters 5, no. 1 (2013): 295-298. https://doi.org/10.3892/ol.2012.984
Copy and paste a formatted citation
x
Spandidos Publications style
Mendoza G, Portillo A and Olmos‑Soto J: Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies. Oncol Lett 5: 295-298, 2013.
APA
Mendoza, G., Portillo, A., & Olmos‑Soto, J. (2013). Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies. Oncology Letters, 5, 295-298. https://doi.org/10.3892/ol.2012.984
MLA
Mendoza, G., Portillo, A., Olmos‑Soto, J."Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies". Oncology Letters 5.1 (2013): 295-298.
Chicago
Mendoza, G., Portillo, A., Olmos‑Soto, J."Accurate breast cancer diagnosis through real-time PCR her‑2 gene quantification using immunohistochemically-identified biopsies". Oncology Letters 5, no. 1 (2013): 295-298. https://doi.org/10.3892/ol.2012.984
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