Pomolic acid induces apoptosis in SK-OV-3 human ovarian adenocarcinoma cells through the mitochondrial-mediated intrinsic and death receptor-induced extrinsic pathways

Yoo,Ki  Hyun; Park,Jong-Hwa; Lee,Do  Kyung; Fu,Yuan  Yuan; Baek,Nam  In; Chung,In  Sik

doi:10.3892/ol.2012.985

Pomolic acid induces apoptosis in SK-OV-3 human ovarian adenocarcinoma cells through the mitochondrial-mediated intrinsic and death receptor-induced extrinsic pathways

Authors:
- Ki Hyun Yoo
- Jong-Hwa Park
- Do Kyung Lee
- Yuan Yuan Fu
- Nam In Baek
- In Sik Chung
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Affiliations: Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Republic of Korea, Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea
Published online on: October 22, 2012 https://doi.org/10.3892/ol.2012.985
Pages: 386-390

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Abstract

The cytotoxic effect of pomolic acid (PA), a pentacyclic triterpene isolated from flowers of Osmanthus fragrans var. aurantiacus Makino, was investigated in SK-OV-3 human ovarian adenocarcinoma cells. PA dose-dependently inhibited the viability of SK-OV-3 cells. PA-induced apoptosis was further characterized by detection of cell surface annexin V and sub-G1 apoptotic cell populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate (FITC) increased following treatment with PA. The sub-G1 cell populations also increased in PA-treated SK-OV-3 cells. PA induced the activation of caspase-8, -9 and -3, critical mediators of apoptosis signaling. PA decreased the mitochondrial transmembrane potential (∆Ψm), resulting in the activation of caspase-9. In addition, PA increased the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis signaling-related death receptor 5 (DR5), mediating caspase-8-involved extrinsic pathway. Taken together, our results indicate that PA induces apoptosis in SK-OV-3 cells, which is mediated by the mitochondrial-mediated intrinsic and death receptor-induced extrinsic pathways.

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