Expression of β-adrenergic receptors in pediatric malignant brain tumors

  • Authors:
    • Iacopo Sardi
    • Laura Giunti
    • Cecilia Bresci
    • Anna Maria Buccoliero
    • Duccio Degl'innocenti
    • Stefania Cardellicchio
    • Gianna Baroni
    • Francesca Castiglione
    • Martina Da Ros
    • Patrizio Fiorini
    • Sabrina  Giglio
    • Lorenzo Genitori
    • Maurizio Aricò
    • Luca Filippi
  • View Affiliations

  • Published online on: October 23, 2012     https://doi.org/10.3892/ol.2012.989
  • Pages: 221-225
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Abstract

β-adrenergic receptors (β-ARs) are G protein‑coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, -9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.
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January 2013
Volume 5 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A.M., Degl'innocenti, D., Cardellicchio, S. ... Filippi, L. (2013). Expression of β-adrenergic receptors in pediatric malignant brain tumors. Oncology Letters, 5, 221-225. https://doi.org/10.3892/ol.2012.989
MLA
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A. M., Degl'innocenti, D., Cardellicchio, S., Baroni, G., Castiglione, F., Da Ros, M., Fiorini, P., Giglio, S., Genitori, L., Aricò, M., Filippi, L."Expression of β-adrenergic receptors in pediatric malignant brain tumors". Oncology Letters 5.1 (2013): 221-225.
Chicago
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A. M., Degl'innocenti, D., Cardellicchio, S., Baroni, G., Castiglione, F., Da Ros, M., Fiorini, P., Giglio, S., Genitori, L., Aricò, M., Filippi, L."Expression of β-adrenergic receptors in pediatric malignant brain tumors". Oncology Letters 5, no. 1 (2013): 221-225. https://doi.org/10.3892/ol.2012.989