Expression of β-adrenergic receptors in pediatric malignant brain tumors

  • Authors:
    • Iacopo Sardi
    • Laura Giunti
    • Cecilia Bresci
    • Anna Maria Buccoliero
    • Duccio Degl'innocenti
    • Stefania Cardellicchio
    • Gianna Baroni
    • Francesca Castiglione
    • Martina Da Ros
    • Patrizio Fiorini
    • Sabrina  Giglio
    • Lorenzo Genitori
    • Maurizio Aricò
    • Luca Filippi
  • View Affiliations

  • Published online on: October 23, 2012     https://doi.org/10.3892/ol.2012.989
  • Pages: 221-225
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Abstract

β-adrenergic receptors (β-ARs) are G protein‑coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, -9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.

References

1 

Daly CJ and McGrath JC: Previously unsuspected widespread cellular and tissue distribution of β-adrenoceptors and its relevance to drug action. Trends Pharmacol Sci. 32:219–226. 2011.PubMed/NCBI

2 

Drell TL IV, Joseph J, Lang K, Niggemann B, Zaenker KS and Entschladen F: Effects of neurotransmitters on the chemokinesis and chemotaxis of MDA-MB-468 human breast carcinoma cells. Breast Cancer Res Treat. 80:63–70. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Masur K, Niggemann B, Zanker KS and Entschladen F: Norepinephrine-induced migration of SW 480 colon carcinoma cells is inhibited by beta-blockers. Cancer Res. 61:2866–2869. 2001.PubMed/NCBI

4 

Palm D, Lang K, Niggemann B, Drell TL IV, Masur K, Zaenker KS and Entschladen F: The norepinephrine-driven metastasis development of PC-3 human prostate cancer cells in BALB/c nude mice is inhibited by beta-blockers. Int J Cancer. 118:2744–2749. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Al-Wadei HA, Al-Wadei MH and Schuller HM: Prevention of pancreatic cancer by the beta-blocker propranolol. Anticancer Drugs. 20:477–482. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Sood AK, Bhatty R, Kamat AA, et al: Stress hormone-mediated invasion of ovarian cancer cells. Clin Cancer Res. 12:369–375. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Yang EV, Kim SJ, Donovan EL, et al: Norepinephrine upregulates VEGF, IL-8, and IL-6 expression in human melanoma tumor cell lines: implications for stress-related enhancement of tumor progression. Brain Behav Immun. 23:267–275. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Schuller HM: Is cancer triggered by altered signalling of nicotinic acetylcholine receptors? Nat Rev Cancer. 9:195–205. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Sloan EK, Priceman SJ, Cox BF, et al: The sympathetic nervous system induces a metastatic switch in primary breast cancer. Cancer Res. 70:7042–7052. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Algazi M, Plu-Bureau G, Flahault A, Dondon MG and Lê MG: Could treatments with beta-blockers be associated with a reduction in cancer risk? Rev Epidemiol Sante Publique. 52:53–65. 2004.(In French).

11 

Perron L, Bairati I, Harel F and Meyer F: Antihypertensive drug use and the risk of prostate cancer (Canada). Cancer Causes Control. 15:535–541. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Powe DG, Voss MJ, Zänker KS, Habashy HO, Green AR, Ellis IO and Entschladen F: Beta-blocker drug therapy reduces secondary cancer formation in breast cancer and improves cancer specific survival. Oncotarget. 1:628–638. 2010.PubMed/NCBI

13 

Ganz PA, Habel LA, Weltzien EK, Caan BJ and Cole SW: Examining the influence of beta blockers and ACE inhibitors on the risk for breast cancer recurrence: results from the LACE cohort. Breast Cancer Res Treat. 129:546–556. 2011.PubMed/NCBI

14 

Barron TI, Connolly RM, Sharp L, Bennett K and Visvanathan K: Beta blockers and breast cancer mortality: a population-based study. J Clin Oncol. 29:2635–2644. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Melhem-Bertrandt A, Chavez-Macgregor M, Lei X, et al: Beta-blocker use is associated with improved relapse-free survival in patients with triple-negative breast cancer. J Clin Oncol. 29:2645–2652. 2011. View Article : Google Scholar : PubMed/NCBI

16 

De Giorgi V, Grazzini M, Gandini S, Benemei S, Lotti T, Marchionni N and Geppetti P: Treatment with β-blockers and reduced disease progression in patients with thick melanoma. Arch Intern Med. 171:779–781. 2011.

17 

Lemeshow S, Sørensen HT, Phillips G, et al: β-Blockers and survival among Danish patients with malignant melanoma: a population-based cohort study. Cancer Epidemiol Biomarkers Prev. 20:2273–2279. 2011.

18 

Park SY, Kang JH, Jeong KJ, et al: Norepinephrine induces VEGF expression and angiogenesis by a hypoxia-inducible factor-1α protein-dependent mechanism. Int J Cancer. 128:2306–2316. 2011.PubMed/NCBI

19 

Magnoni MS, Frattola L, Piolti R, Govoni S, Kobayashi H and Trabucchi M: Glial brain tumors lack microvascular adrenergic receptors. Eur Neurol. 28:27–29. 1988. View Article : Google Scholar : PubMed/NCBI

20 

Ruck A, Millns P, Kendall DA and Hill SJ: Expression of beta 2-adrenoceptors mediating cyclic AMP accumulation in astroglial and neuronal cell lines derived from the rat CNS. Biochem Pharmacol. 40:2371–2375. 1990. View Article : Google Scholar : PubMed/NCBI

21 

Sokołowska P and Nowak JZ: Constitutive activity of beta-adrenergic receptors in C6 glioma cells. Pharmacol Rep. 57:659–663. 2005.PubMed/NCBI

22 

Lung HL, Shan SW, Tsang D and Leung KN: Tumor necrosis factor-alpha mediates the proliferation of rat C6 glioma cells via beta-adrenergic receptors. J Neuroimmunol. 166:102–112. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Annabi B, Lachambre MP, Plouffe K, Moumdjian R and Béliveau R: Propranolol adrenergic blockade inhibits human brain endothelial cells tubulogenesis and matrix metalloproteinase-9 secretion. Pharmacol Res. 60:438–445. 2009. View Article : Google Scholar

24 

Annabi B, Vaillancourt-Jean E, Weil AG and Béliveau R: Pharmacological targeting of β-adrenergic receptor functions abrogates NF-κB signaling and MMP-9 secretion in medulloblastoma cells. Onco Targets Ther. 3:219–226. 2010.

25 

Toll L, Jimenez L, Waleh N, et al: {Beta}2-adrenergic receptor agonists inhibit the proliferation of 1321N1 astrocytoma cells. J Pharmacol Exp Ther. 336:524–532. 2011.

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Chakroborty D, Sarkar C, Basu B, Dasgupta PS and Basu S: Catecholamines regulate tumor angiogenesis. Cancer Res. 69:3727–3730. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Zhang D, Ma Q, Shen S and Hu H: Inhibition of pancreatic cancer cell proliferation by propranolol occurs through apoptosis induction: the study of beta-adrenoceptor antagonist’s anticancer effect in pancreatic cancer cell. Pancreas. 38:94–100. 2009.PubMed/NCBI

29 

Pasquier E, Ciccolini J, Carre M, et al: Propranolol potentiates the anti-angiogenic effects and anti-tumor efficacy of chemotherapy agents: implication in breast cancer treatment. Oncotarget. 2:797–809. 2011.PubMed/NCBI

30 

Entschladen F, Drell TL IV, Lang K, Joseph J and Zaenker KS: Tumour-cell migration, invasion, and metastasis: navigation by neurotransmitters. Lancet Oncol. 5:254–258. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Entschladen F, Drell TL IV, Lang K, Joseph J and Zaenker KS: Neurotransmitters and chemokines regulate tumor cell migration: potential for a new pharmacological approach to inhibit invasion and metastasis development. Curr Pharm Des. 11:403–411. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Diaz E, Karlan B, Cass I, Walsh C and Li A: Impact of beta blockers on epithelial ovarian cancer survival. Gynecol Oncol. 120(Suppl 1): S362011. View Article : Google Scholar

33 

Shakhar G and Ben-Eliyahu S: In vivo beta-adrenergic stimulation suppresses natural killer activity and compromises resistance to tumor metastasis in rats. J Immunol. 160:3251–3258. 1998.PubMed/NCBI

34 

Lakka SS, Gondi CS and Rao JS: Proteases and glioma angiogenesis. Brain Pathol. 15:327–341. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Ristori C, Filippi L, Dal Monte M, et al: Role of the adrenergic system in a mouse model of oxygen-induced retinopathy: antiangiogenic effects of beta-adrenoreceptor blockade. Invest Ophthalmol Vis Sci. 52:155–170. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
APA
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A.M., Degl'innocenti, D., Cardellicchio, S. ... Filippi, L. (2013). Expression of β-adrenergic receptors in pediatric malignant brain tumors. Oncology Letters, 5, 221-225. https://doi.org/10.3892/ol.2012.989
MLA
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A. M., Degl'innocenti, D., Cardellicchio, S., Baroni, G., Castiglione, F., Da Ros, M., Fiorini, P., Giglio, S., Genitori, L., Aricò, M., Filippi, L."Expression of β-adrenergic receptors in pediatric malignant brain tumors". Oncology Letters 5.1 (2013): 221-225.
Chicago
Sardi, I., Giunti, L., Bresci, C., Buccoliero, A. M., Degl'innocenti, D., Cardellicchio, S., Baroni, G., Castiglione, F., Da Ros, M., Fiorini, P., Giglio, S., Genitori, L., Aricò, M., Filippi, L."Expression of β-adrenergic receptors in pediatric malignant brain tumors". Oncology Letters 5, no. 1 (2013): 221-225. https://doi.org/10.3892/ol.2012.989