Pseudomonas aeruginosa‑mannose‑sensitive hemagglutinin inhibits proliferation and induces apoptosis in a caspase‑dependent manner in human bladder cancer cell lines

Zhu,Yi‑Ping; Bian,Xiao‑Jie; Ye,Ding‑Wei; Yao,Xu‑Dong; Zhang,Shi‑Lin; Dai,Bo; Shen,Yi‑Jun

doi:10.3892/ol.2013.1201

Pseudomonas aeruginosa‑mannose‑sensitive hemagglutinin inhibits proliferation and induces apoptosis in a caspase‑dependent manner in human bladder cancer cell lines

Authors:
- Yi‑Ping Zhu
- Xiao‑Jie Bian
- Ding‑Wei Ye
- Xu‑Dong Yao
- Shi‑Lin Zhang
- Bo Dai
- Yi‑Jun Shen
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Affiliations: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China
Published online on: February 19, 2013 https://doi.org/10.3892/ol.2013.1201
Pages: 1357-1362

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Abstract

The aim of the present study was to investigate the effects of Pseudomonas aeruginosa‑mannose‑sensitive hemagglutinin (PA‑MSHA) on inhibiting the proliferation of bladder cancer cell lines and to further deﬁne its functional mechanisms. T24 and 5637 cells were treated with PA‑MSHA at various concentrations and times. Cell proliferation was analyzed using Cell Counting Kit‑8 (CCK‑8) assays. The cell cycle distribution and apoptosis induced by PA‑MSHA were measured by flow cytometry with propidium iodide (PI) and annexin V‑fluorescein isothiocyanate (FITC) staining. Western blotting was used to evaluate the expression levels of the apoptosis‑related molecules and PI3K‑AKT‑mTOR signaling pathway proteins. A time‑ and concentration‑dependent cytotoxic effect of PA‑MSHA was observed in the T24 and 5637 cells. Flow cytometry with PI and annexin V‑FITC staining showed that the various concentrations of PA‑MSHA were all able to induce the apoptosis and G0‑G1 cell cycle arrest of the bladder cancer cells. Cleaved caspase‑8 and ‑9 and Fas protein expression levels were markedly associated with an increase in the apoptosis of the bladder cancer cells. The cells stimulated with PA‑MSHA also exhibited a downregulation of PI3K‑AKT‑mTOR signaling. PA‑MSHA inhibits proliferation and induces apoptosis in the T24 and 5637 bladder cancer cell lines by modulating caspase family proteins and affecting the cell cycle regulation machinery. The PI3K‑AKT‑mTOR signaling pathway may be important in the direct anticancer cytotoxic effect of PA‑MSHA.

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