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Article

Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient

  • Authors:
    • Walid Al-Achkar
    • Abdulsamad Wafa
    • Faten Moassass
    • Elisabeth Klein
    • Thomas Liehr
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria, Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, D-07743 Jena, Germany
  • Pages: 1579-1582
    |
    Published online on: March 5, 2013
       https://doi.org/10.3892/ol.2013.1225
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Abstract

The so-called Philadelphia (Ph) chromosome is present in more than 90% of chronic myeloid leukemia (CML) cases. Amplification or duplication of the BCR-ABL gene has been found to be one of the key factors leading to drug resistance to imatinib mesylate (IM). In the present study, we identified the presence of isodicentric Ph chromosomes [idic(Ph)] in an IM-resistant patient. Fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes confirmed the heterogeneity and amplification of the fused BCR-ABL gene. FISH analysis superimposed on G-banding confirmed the presence of idic(Ph) chromosomes. Reverse transcription-polymerase chain reaction (RT-PCR) products revealed the presence of the BCR-ABL fusion transcript b3a2. The idic(Ph) chromosomes in CML were shown to be fused at the satellite regions of the short arms. The patient did not respond to IM chemotherapy and did not achieve remission. In this study, the impact of the idic(Ph) chromosomes on genomic instability, heterogeneity and amplification of the BCR-ABL gene in IM-resistant patients is discussed.
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Copy and paste a formatted citation
Spandidos Publications style
Al-Achkar W, Wafa A, Moassass F, Klein E and Liehr T: Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient. Oncol Lett 5: 1579-1582, 2013.
APA
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., & Liehr, T. (2013). Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient. Oncology Letters, 5, 1579-1582. https://doi.org/10.3892/ol.2013.1225
MLA
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., Liehr, T."Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient". Oncology Letters 5.5 (2013): 1579-1582.
Chicago
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., Liehr, T."Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient". Oncology Letters 5, no. 5 (2013): 1579-1582. https://doi.org/10.3892/ol.2013.1225
Copy and paste a formatted citation
x
Spandidos Publications style
Al-Achkar W, Wafa A, Moassass F, Klein E and Liehr T: Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient. Oncol Lett 5: 1579-1582, 2013.
APA
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., & Liehr, T. (2013). Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient. Oncology Letters, 5, 1579-1582. https://doi.org/10.3892/ol.2013.1225
MLA
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., Liehr, T."Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient". Oncology Letters 5.5 (2013): 1579-1582.
Chicago
Al-Achkar, W., Wafa, A., Moassass, F., Klein, E., Liehr, T."Multiple copies of BCR-ABL fusion gene on two isodicentric Philadelphia chromosomes in an imatinib mesylate‑resistant chronic myeloid leukemia patient". Oncology Letters 5, no. 5 (2013): 1579-1582. https://doi.org/10.3892/ol.2013.1225
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