Anticancer effects of 4-vinyl-2,6-dimethoxyphenol (canolol) against SGC-7901 human gastric carcinoma cells

Jiang,Jing; Cao,Dong-Hui; Tsukamoto,Tetsuya; Wang,Guo‑Qing; Jia,Zhi-Fang; Suo,Jian; Cao,Xue-Yuan

doi:10.3892/ol.2013.1230

Anticancer effects of 4-vinyl-2,6-dimethoxyphenol (canolol) against SGC-7901 human gastric carcinoma cells

Authors:
- Jing Jiang
- Dong-Hui Cao
- Tetsuya Tsukamoto
- Guo‑Qing Wang
- Zhi-Fang Jia
- Jian Suo
- Xue-Yuan Cao
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Affiliations: Division of Clinical Epidemiology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Division of Pathology, School of Medicine, Fujita Health University, Toyoake, Japan, Department of Pathogeny Biology, Norman Bethune Medical College of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gastric and Colorectal Surgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: March 5, 2013 https://doi.org/10.3892/ol.2013.1230
Pages: 1562-1566

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Abstract

Gastric cancer remains the fourth most commonly diagnosed cancer and is the second leading cause of cancer-related mortality worldwide. The aim of this study was to investigate the effects of canolol on the proliferation and apoptosis of SGC-7901 human gastric cancer cells and its relevant molecular mechanisms. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to observe the effect of canolol on the proliferation of SGC-7901 human gastric adenocarcinoma cells. The results showed that SGC-7901 cells exhibited a marked dose-dependent reduction in the proliferation rate. The survival rate of the cells was 88.86±1.58% at 50 µmol/l, decreasing to 53.73±1.51% at 800 µmol/l (P<0.05). By contrast, canolol had no significant toxicity on the human gastric mucosal epithelial cell line GES-1. The vivid images of cell morphology using an inverted microscope provided confirmation of the MTT assay. Treatment of SGC-7901 cells with canolol resulted in apoptosis demonstrated by flow cytometry. Furthermore, canolol downregulated the mRNA levels of COX-2, but had no significant effect on the mRNA expession of the Bax and Bcl-2 genes. These findings suggest that canolol has potential to be developed as a new natural anti-gastric carcinoma agent.

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1	Ferlay J, Shin H R, Bray F, et al: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI
2	Liu X, Zhang B, Guo Y, et al: Down-regulation of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human gastric cancer cells. PLoS ONE. 7:e370962012. View Article : Google Scholar : PubMed/NCBI
3	Chuah SK, Tsay FW, Hsu PI and Wu DC: A new look at anti-Helicobacter pylori therapy. World J Gastroenterol. 17:3971–3975. 2011. View Article : Google Scholar : PubMed/NCBI
4	Graham D Y: Antibiotic resistance in Helicobacter pylori: implications for therapy. Gastroenterology. 115:1272–1277. 1998.
5	Siegel R, Naishadham D and Jemal A: Cancer statistics. CA Cancer J Clin. 62:10–29. 2012.
6	Kountouri AM, Kaliora AC, Koumbi L and Andrikopoulos NK: In-vitro gastric cancer prevention by a polyphenol-rich extract from olives through induction of apoptosis. Eur J Cancer Prev. 18:33–39. 2009. View Article : Google Scholar : PubMed/NCBI
7	Alvarez-Suarez JM, Dekanski D, Ristić S, et al: Strawberry polyphenols attenuate ethanol-induced gastric lesions in rats by activation of antioxidant enzymes and attenuation of MDA increase. PLoS One. 6:e258782011. View Article : Google Scholar
8	Jaganathan SK and Supriyanto E: Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells. Molecules. 17:6290–6304. 2012. View Article : Google Scholar : PubMed/NCBI
9	Vaux DL and Korsmeyer SJ: Cell death in development. Cell. 96:245–254. 1999. View Article : Google Scholar
10	Cao XY, Tsukamoto T, Seki T, et al: 4-Vinyl-2,6-dimethoxyphenol (canolol) suppresses oxidative stress and gastric carcinogenesis in Helicobacter pylori-infected carcinogen-treated Mongolian gerbils. Int J Cancer. 122:1445–1454. 2008. View Article : Google Scholar : PubMed/NCBI
11	Harris RE, Chlebowski RT, Jackson RD, et al: Breast cancer and nonsteroidal anti-inflammatory drugs: prospective results from the Women’s Health Initiative. Cancer Res. 63:6096–6101. 2003.
12	Kilic G, Gurates B, Garon J, et al: Expression of cyclooxygenase-2 in endometrial adenocarcinoma. Eur J Gynaecol Oncol. 26:271–274. 2005.PubMed/NCBI
13	Targosz A, Brzozowski T, Pierzchalski P, Szczyrk U, Ptak-Belowska A, Konturek SJ and Pawlik W: Helicobacter pylori promotes apoptosis, activates cyclooxygenase (COX)-2 and inhibits heat shock protein HSP70 in gastric cancer epithelial cells. Inflamm Res. 61:955–966. 2012. View Article : Google Scholar
14	Ashok V, Dash C, Rohan TE, Sprafka JM and Terry PD: Selective cyclooxygenase-2 (COX-2) inhibitors and breast cancer risk. Breast. 20:66–70. 2011. View Article : Google Scholar : PubMed/NCBI
15	Ozalp SS, Eren CY, Bostancioğlum RB and Koparal AT: Induction of apoptosis and inhibition of cell proliferation by the cyclooxygenase enzyme blocker-nimesulide in the Ishikawa endometrial cancer cell line. Eur J Obstet Gyn Reprod Biol. 164:79–84. 2012. View Article : Google Scholar : PubMed/NCBI
16	Sun SY, Hail JRN and Lotan R: Apoptosis as a novel target for cancer chemoprevention. J Natl Cancer Inst. 96:662–672. 2004. View Article : Google Scholar : PubMed/NCBI
17	Tripathi M, Singh BK, Mishra C, Raisuddin S and Kakkar P: Involvement of mitochondria mediated pathways in hepato-protection conferred by Fumaria parviflora Lam. Extract against nimesulide induced apoptosis in vitro. Toxicol In Vitro. 24:495–508. 2010. View Article : Google Scholar : PubMed/NCBI
18	Wu XY, Wang YL, Wang HW, et al: Quinacrine inhibits cell growth and induces apoptosis in human gastric cancer cell line SGC-7901. Cur Ther Res Clin Exp. 73:52–64. 2012. View Article : Google Scholar : PubMed/NCBI
19	Ji YB, Qu ZY and Zou X: Juglone-induced apoptosis in human gastric cancer SGC-7901 cells via the mitochondrial pathway. Exp Toxicol Pathol. 63:69–78. 2011. View Article : Google Scholar : PubMed/NCBI
20	Harris RE, Beebe J and Alshafie GA: Reduction in cancer risk by selective and nonselective cyclooxygenase-2 (COX-2) inhibitors. J Exp Pharmacol. 6:491–496. 2012.
21	Dong X, Li ZR, Wang W, Zhang WJ, Liu SZ and Zhang XM: Protective effect of canolol from oxidative stress-induced cell damage in ARPE-19 cells via an ERK mediated antioxidative pathway. Mol Vis. 17:2040–2048. 2011.PubMed/NCBI
22	Jia N, Xiong YLL, Kong BH, Liu Q and Xia XF: Radical scavenging activity of black currant (Ribes nigrum L) extract and its inhibitory effect on gastric cancer cell proliferation via induction of apoptosis. J Funct Foods. 4:382–390. 2011.
23	Gao LL, Li FR, Jiao P, et al: Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells. World J Gastroenterol. 17:4389–4395. 2011. View Article : Google Scholar : PubMed/NCBI
24	Hu MM, Xu LN, Yin LH, et al: Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways. J Appl Toxicol. View Article : Google Scholar : 2012.PubMed/NCBI
25	Fu SL, Wu YL, Zhang YP, Qiao MM and Chen Y: Anti-cancer effects of COX-2 inhibitors and their correlation with angio-genesis and invasion in gastric cancer. World J Gastroenterol. 10:1971–1974. 2004.PubMed/NCBI
26	Harris RE: Cyclooxygenase-2 (COX-2) blockade in the chemoprevention of cancers of the colon, breast, prostate, and lung. Inflammopharmacology. 17:55–67. 2009. View Article : Google Scholar : PubMed/NCBI
27	Chan AT, Ogino S and Fuchs CS: Aspirin use and survival after diagnosis of colorectal cancer. JAMA. 302:649–658. 2010. View Article : Google Scholar : PubMed/NCBI
28	Ma X, Kundu N, Rifat S, Walser T and Fulton AM: Prostaglandin E receptor EP4 antagonism inhibits breast cancer metastasis. Cancer Res. 66:2923–2927. 2006. View Article : Google Scholar : PubMed/NCBI
29	Tsujimoto Y and Shimizu S: Bcl-2 family: life-or-death switch. FEBS Lett. 466:6–10. 2000. View Article : Google Scholar : PubMed/NCBI
30	Tjiu JW, Liao YH, Lin SJ, et al: Cyclooxygenase-2 overexpression in human basal cell carcinoma cell line increases antiapoptosis, angiogenesis, and tumorigenesis. J Invest Dermatol. 126:1143–1151. 2006. View Article : Google Scholar : PubMed/NCBI
31	Liu CH, Chang SH, Narko K, et al: Overexpression of cyclooxygenase-2 is sufficient to induce tumorigenesis in transgenic mice. J Biol Chem. 276:18563–18569. 2001. View Article : Google Scholar : PubMed/NCBI