Desmoplastic small round cell tumor (DSRCT) xenografts and tissue culture lines: Establishment and initial characterization

Markides,Constantine  S.A.; Coil,Douglas  R.; Luong,Linh  H.; Mendoza,John; Kozielski,Tony; Vardeman,Dana; Giovanella,Beppino  C.

doi:10.3892/ol.2013.1265

May 2013 Volume 5 Issue 5

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May 2013 Volume 5 Issue 5

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Article

Desmoplastic small round cell tumor (DSRCT) xenografts and tissue culture lines: Establishment and initial characterization

Authors:
- Constantine S.A. Markides
- Douglas R. Coil
- Linh H. Luong
- John Mendoza
- Tony Kozielski
- Dana Vardeman
- Beppino C. Giovanella
View Affiliations / Copyright

Affiliations: Christus Stehlin Foundation for Cancer Research, Houston, TX 77025, USA
Pages: 1453-1456
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Published online on: March 19, 2013

https://doi.org/10.3892/ol.2013.1265
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Abstract

Desmoplastic small round cell tumor (DSRCT) is an extremely rare and aggressive neoplasm, which mainly affects young males and generally presents as a widely disseminated tumor within the peritoneal cavity. Due to the rarity of the tumor, its younger and overall healthier patient population (compared with other tumor types) and the fact that it lacks definitive histological and immunohistological features, the diagnosis of DSRCT may be frequently delayed or the tumor may be entirely misdiagnosed as a different type of abdominal sarcoma. The present study aimed to rectify the lack of models that exist for this rare neoplasm, through the development of several DSRCT tissue cultures and xenograft lines. Samples were received from surgeries and biopsies from patients worldwide and were immediately processed for xenograft development in nude mice. Tumor tissues were minced and fragments were injected into the dorsal flanks of nude mice. Of the 14 samples received, nine were established into xenograft lines and five into tissue culture lines. Xenografts displayed the microscopic histology of their parent tumors and demonstrated two different growth rates among the established xenograft lines. Overall, the establishment of these xenograft and tissue culture lines provides researchers with tools to evaluate DSRCT responses to chemotherapy and to investigate DSRCT‑specific signaling pathways or mechanisms.

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1	Gerald WL and Rosai J: Case 2. Desmoplastic small cell tumor with divergent differentiation. Pediatr Pathol. 9:177–183. 1989. View Article : Google Scholar : PubMed/NCBI
2	Cummings OW, Ulbright TM, Young RH, Dei Tos AP, Fletcher CD and Hull MT: Desmoplastic small round cell tumors of the paratesticular region. A report of six cases. Am J Surg Pathol. 21:219–225. 1997. View Article : Google Scholar : PubMed/NCBI
3	Parkash V, Gerald WL, Parma A, Miettinen M and Rosai J: Desmoplastic small round cell tumor of the pleura. Am J Surg Pathol. 19:659–665. 1995. View Article : Google Scholar : PubMed/NCBI
4	Tison V, Cerasoli S, Morigi F, Ladanyi M, Gerald WL and Rosai J: Intracranial desmoplastic small-cell tumor. Report of a case. Am J Surg Pathol. 20:112–117. 1996. View Article : Google Scholar : PubMed/NCBI
5	Adsay V, Cheng J, Athanasian E, Gerald W and Rosai J: Primary desmoplastic small cell tumor of soft tissues and bone of the hand. Am J Surg Pathol. 23:1408–1413. 1999. View Article : Google Scholar : PubMed/NCBI
6	Young RH, Eichhorn JH, Dickersin GR and Scully RE: Ovarian involvement by the intra-abdominal desmoplastic small round cell tumor with divergent differentiation: a report of three cases. Hum Pathol. 23:454–464. 1992. View Article : Google Scholar : PubMed/NCBI
7	Wolf AN, Ladanyi M, Paull G, Blaugrund JE and Westra WH: The expanding clinical spectrum of desmoplastic small round-cell tumor: a report of two cases with molecular confirmation. Hum Pathol. 30:430–435. 1999. View Article : Google Scholar : PubMed/NCBI
8	Syed S, Haque AK, Hawkins HK, Sorensen PH and Cowan DF: Desmoplastic small round cell tumor of the lung. Arch Pathol Lab Med. 126:1226–1228. 2002.PubMed/NCBI
9	Chang F: Desmoplastic small round cell tumors: cytologic, histologic, and immunohistochemical features. Arch Pathol Lab Med. 130:728–732. 2006.PubMed/NCBI
10	Sawyer JR, Tryka AF and Lewis JM: A novel reciprocal chromosome translocation t(11;22)(p13;q12) in an intraabdominal desmoplastic small round-cell tumor. Am J Surg Pathol. 16:411–416. 1992. View Article : Google Scholar : PubMed/NCBI
11	Mrabti H, Kaikani W, Ahbeddou N, Abahssain H, El Khannoussi B, Amrani M and Errihani H: Metastatic desmoplastic small round cell tumor controlled by an anthracycline-based regimen: review of the role of chemotherapy. J Gastrointest Cancer. 43:103–109. 2012. View Article : Google Scholar : PubMed/NCBI
12	Shao ZM, Nguyen M and Barsky SH: Human breast carcinoma desmoplasia is PDGF initiated. Oncogene. 19:4337–4345. 2000. View Article : Google Scholar : PubMed/NCBI
13	Schmid SA, Dietrich A, Schulte S, Gaumann A and Kunz-Schughart LA: Fibroblastic reaction and vascular maturation in human colon cancers. Int J Radiat Biol. 85:1013–1025. 2009. View Article : Google Scholar : PubMed/NCBI
14	Schwartz RN, Blanke CD and Pesko LJ: Targeted therapies in the treatment of colorectal cancer: what managed care needs to know. J Manag Care Pharm. 10(5 Suppl B): S2–S13. 2004.PubMed/NCBI
15	Bartlett NL, Johnson JL, Wagner-Johnston N, Ratain MJ and Peterson BA; Cancer and Leukemia Group B: Phase II study of 9-aminocamptothecin in previously treated lymphomas: results of Cancer and Leukemia Group B 9551. Cancer Chemother Pharmacol. 63:793–798. 2009. View Article : Google Scholar : PubMed/NCBI
16	Sarris AH, Phan A, Goy A, Romaguera J, Hagemeister FB, Rodriguez MA, McLaughlin P, Pro B, Medeiros LJ, Samuels B, Mesina O, Bleyer AW and Cabanillas F: Irinotecan in relapsed or refractory non-Hodgkin’s lymphomas. Indications of activity in a phase II trial. Oncology (Williston Park). 16(8 Suppl 7): 27–31. 2002.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Desmoplastic small round cell tumor (DSRCT) xenografts and tissue culture lines: Establishment and initial characterization

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