Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June 2013 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer

  • Authors:
    • Xiao‑Guang Lv
    • Xiao‑Fei Lei
    • Meng‑Yao Ji
    • Xu‑Feng Guo
    • Jing Wang
    • Wei‑Guo Dong
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 1844-1848
    |
    Published online on: March 26, 2013
       https://doi.org/10.3892/ol.2013.1271
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ezrin‑radixin‑moesin‑binding phosphoprotein 50 (EBP50) is a postsynaptic density‑95⁄disc‑large⁄zonula occludens‑1 (PDZ) homologous domain‑containing protein that is involved in cell signaling. EBP50 regulates cell apoptosis, proliferation and invasion. In the present study, the prognostic impact factor of EBP50 expression was evaluated using a quantum dot (QD)‑based assay and immunohistochemistry (IHC). The EBP50 protein expression in gastric cancer (GC) tissues was evaluated using IHC and QD‑IHC. The study included 101 patients with GC (29 females and 72 males, aged 24‑81 years), diagnosed and treated at the General Surgery Department of Renmin Hospital of Wuhan University (Wuhan, China) between 2000 and 2005. The survival rate was calculated using the Kaplan‑Meier method and log‑rank tests. IHC and QD analyses of 101 GC tissue specimens revealed that EBP50‑positive tumor cells were frequently present in GC. Increased EBP50 immunostaining was observed in 63 specimens (62.4%). The EBP50 expression levels were correlated with increased tumor size and the male gender. EBP50 was well distributed in the cytoplasm and nuclei of the GC cells. However, EBP50 protein expression exhibited no correlation with age, differentiation, stage or lymph node metastasis. There were no associations between the expression of EBP50 and the mean survival rates (IHC, 50.5 vs. 58.1 months, P>0.05; QD, 55.4 vs. 63.2 months, P>0.05). These findings suggest that EBP50 protein expression is not correlated with the prognosis of patients with GC. QD‑IHC and IHC have similar advantages for the detection of EBP50 protein expression.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Hartgrink HH, Jansen EP, van Grieken NC and van de Velde CJ: Gastric cancer. Lancet. 374:477–490. 2009. View Article : Google Scholar

2 

Kodera Y, Yamamura Y, Torii A, Uesaka K, Hirai T, Yasui K, et al: The prognostic value of preoperative serum levels of CEA and CA19-9 in patients with gastric cancer. Am J Gastroenterol. 91:49–53. 1996.PubMed/NCBI

3 

Tocchi A, Costa G, Lepre L, Liotta G, Mazzoni G, Cianetti A and Vannini P: The role of serum and gastric juice levels of carcinoembryonic antigen, CA19.9 and CA72.4 in patients with gastric cancer. J Cancer Res Clin Oncol. 124:450–455. 1998. View Article : Google Scholar

4 

Hummel R, Hussey DJ and Haier J: MicroRNAs: predictors and modifiers of chemo- and radiotherapy in different tumour types. Eur J Cancer. 46:298–311. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Yamao T, Kai S, Kazami A, Koizumi K, Handa T, Takemoto N and Maruyama M: Tumor markers CEA, CA19-9 and CA125 in monitoring of response to systemic chemotherapy in patients with advanced gastric cancer. Jpn J Clin Oncol. 29:550–555. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Macdonald JS, Smalley SR, Benedetti J, Hundahl SA, Estes NC, Stemmermann GN, et al: Chemoradiotherapy after surgery compared with surgery alone for adenocarcinoma of the stomach or gastroesophageal junction. N Engl J Med. 345:725–730. 2001. View Article : Google Scholar

7 

Ichikawa D, Koike H, Ikoma H, Ikoma D, Tani N, Otsuji E, et al: Detection of aberrant methylation as a tumor marker in serum of patients with gastric cancer. Anticancer Res. 24:2477–2481. 2004.PubMed/NCBI

8 

Tani N, Ichikawa D, Ikoma D, Tomita H, Sai S, Ikoma H, et al: Circulating cell-free mRNA in plasma as a tumor marker for patients with primary and recurrent gastric cancer. Anticancer Res. 27:1207–1212. 2007.PubMed/NCBI

9 

Li N, Zhang J, Liang Y, Shao J, Peng F, Sun M, et al: A controversial tumor marker: is SM22 a proper biomarker for gastric cancer cells? J Proteome Res. 6:3304–3312. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Shibata T, Chuma M, Kokubu A, Sakamoto M and Hirohashi S: EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma. Hepatology. 38:178–186. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Song J, Bai J, Yang W, Gabrielson EW, Chan DW and Zhang Z: Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer. Histopathology. 51:40–53. 2007. View Article : Google Scholar

12 

Zheng JF, Sun LC, Liu H, Huang Y, Li Y and He J: EBP50 exerts tumor suppressor activity by promoting cell apoptosis and retarding extracellular signal-regulated kinase activity. Amino Acids. 38:1261–1268. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Bellizzi A, Mangia A, Malfettone A, Cardone RA, Simone G, Reshkin SJ and Paradiso A: Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour. Histopathology. 58:1086–1095. 2011.

14 

Ingraffea J, Reczek D and Bretscher A: Distinct cell type-specific expression of scaffolding proteins EBP50 and E3KARP: EBP50 is generally expressed with ezrin in specific epithelia, whereas E3KARP is not. Eur J Cell Biol. 81:61–68. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Sobin LH and Fleming ID: TNM Classification of Malignant Tumors, fifth edition (1997). Union Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer. 80:1803–1804. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Hu Y, Wu Q, Liu S, Wei L, Chen X, Yan Z, et al: Study of rice pollen grains by multispectral imaging microscopy. Microsc Res Tech. 68:335–346. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Guo N, Zeng L and Wu Q: A method based on multispectral imaging technique for white blood cell segmentation. Comput Biol Med. 37:70–76. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Dickinson ME, Bearman G, Tille S, Lansford R and Fraser SE: Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy. Biotechniques. 31:12721274–1276. 12782001.PubMed/NCBI

19 

Chen H, Xue J, Zhang Y, Zhu X, Gao J and Yu B: Comparison of quantum dots immunofluorescence histochemistry and conventional immunohistochemistry for the detection of caveolin-1 and PCNA in the lung cancer tissue microarray. J Mol Histol. 40:261–268. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Yao W, Feng D, Bian W, et al: EBP50 inhibits EGF-induced breast cancer cell proliferation by blocking EGFR phosphorylation. Amino Acids. 43:2027–2035. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Kislin KL, McDonough WS, Eschbacher JM, et al: NHERF-1: modulator of glioblastoma cell migration and invasion. Neoplasia. 11:377–387. 2009.PubMed/NCBI

22 

Malfettone A, Saponaro C, Paradiso A, et al: Peritumoral vascular invasion and NHERF1 expression define an immunophenotype of grade 2 invasive breast cancer associated with poor prognosis. BMC Cancer. 12:1062012. View Article : Google Scholar : PubMed/NCBI

23 

Bornschein J and Malfertheiner P: Gastric carcinogenesis. Langenbecks Arch Surg. 396:729–742. 2011. View Article : Google Scholar

24 

Kawachi T, Kurisu M, Numanyu N, Sasajima K and Sano T: Precancerous changes in the stomach. Cancer Res. 36:2673–2677. 1976.PubMed/NCBI

25 

Muthu MS, Kulkarni SA, Raju A and Feng SS: Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots. Biomaterials. 33:3494–3501. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Shao L, Gao Y and Yan F: Semiconductor quantum dots for biomedicial applications. Sensors (Basel). 11:11736–11751. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lv XG, Lei XF, Ji MY, Guo XF, Wang J and Dong WG: Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer. Oncol Lett 5: 1844-1848, 2013.
APA
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., & Dong, W. (2013). Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer. Oncology Letters, 5, 1844-1848. https://doi.org/10.3892/ol.2013.1271
MLA
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., Dong, W."Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer". Oncology Letters 5.6 (2013): 1844-1848.
Chicago
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., Dong, W."Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer". Oncology Letters 5, no. 6 (2013): 1844-1848. https://doi.org/10.3892/ol.2013.1271
Copy and paste a formatted citation
x
Spandidos Publications style
Lv XG, Lei XF, Ji MY, Guo XF, Wang J and Dong WG: Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer. Oncol Lett 5: 1844-1848, 2013.
APA
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., & Dong, W. (2013). Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer. Oncology Letters, 5, 1844-1848. https://doi.org/10.3892/ol.2013.1271
MLA
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., Dong, W."Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer". Oncology Letters 5.6 (2013): 1844-1848.
Chicago
Lv, X., Lei, X., Ji, M., Guo, X., Wang, J., Dong, W."Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer". Oncology Letters 5, no. 6 (2013): 1844-1848. https://doi.org/10.3892/ol.2013.1271
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team