Application of surface‑enhanced laser desorption/ionization time‑of‑flight mass spectrometry technology for the diagnosis of colorectal adenoma

Zhou,Zhong‑Yin; Tao,Di‑Di; Cao,Ji‑Wang; Luo,He‑Sheng

doi:10.3892/ol.2013.1304

June 2013 Volume 5 Issue 6

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June 2013 Volume 5 Issue 6

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Article

Application of surface‑enhanced laser desorption/ionization time‑of‑flight mass spectrometry technology for the diagnosis of colorectal adenoma

Authors:
- Zhong‑Yin Zhou
- Di‑Di Tao
- Ji‑Wang Cao
- He‑Sheng Luo
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Pages: 1935-1938
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Published online on: April 15, 2013

https://doi.org/10.3892/ol.2013.1304
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Abstract

The aim of the present study was to identify a specific biological marker for the diagnosis of colorectal adenomas through the analysis of variations in serum protein profiling in colorectal adenoma patients. The study was conducted at the Renmin Hospital of Wuhan University (Wuhan, China) between September 2011 and May 2012. Surface‑enhanced laser desorption/ionization time‑of‑flight mass spectrometry (SELDI‑TOF‑MS) was performed to compare the serum protein profiles of 50 patients with colorectal adenoma and 50 healthy individuals. The obtained protein profiles were analyzed using Biomarker Wizard software. Twenty protein peaks were identified to exhibit differences in average intensity between colorectal adenomas compared with normal controls, including peaks 8,565.84, 8,694.51 and 5,910.50 Da, in which the intensity between the patients and control individuals was significantly different. Two peaks, 8,565.84 and 8,694.51 Da, were observed to be highly expressed in the colorectal adenomas, however, expression was low in the control samples. By contrast, 5,910.50 Da expression was low in the colorectal adenomas and high in the controls. The results of the current study indicate that the three protein peaks may represent specific biomarkers for colorectal adenomas.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Application of surface‑enhanced laser desorption/ionization time‑of‑flight mass spectrometry technology for the diagnosis of colorectal adenoma

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