Gynura procumbens ethanolic extract suppresses osteosarcoma cell proliferation and metastasis in vitro

Wang,Heng; Zhou,Ji  Wen; Fu,Da  Hua; Zhou,Yang; Cheng,Wen  Zhao; Liu,Zhi‑Li

doi:10.3892/ol.2013.1315

Gynura procumbens ethanolic extract suppresses osteosarcoma cell proliferation and metastasis in vitro

Authors:
- Heng Wang
- Ji Wen Zhou
- Da Hua Fu
- Yang Zhou
- Wen Zhao Cheng
- Zhi‑Li Liu
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Affiliations: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Zhangzhou Health Vocational College, Zhangzhou, Fujian 363000, P.R. China
Published online on: April 22, 2013 https://doi.org/10.3892/ol.2013.1315
Pages: 113-117

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Abstract

Gynura procumbens is a traditional herb used for the treatment of inflammation, rheumatism and viral infections, although the antitumor effect and its potential mechanisms of action remain unclear. In the present study, the antitumor effect of Gynura procumbens ethanolic extract (GPE) on the osteosarcoma (OS) cell line, U2‑OS, was investigated in vitro. Cell proliferation and apoptosis were measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Transwell invasion and wound healing assays were performed to investigate the invasion and migration of the U2‑OS cells. The results showed that GPE was able to inhibit U2‑OS cell proliferation and metastasis and induce cell apoptosis. Furthermore, the expression of the NF‑κBp65 protein was detected by western blotting to evaluate the effects of GPE on the nuclear transfer of NF‑κB. It was demonstrated that the expression of the NF‑κBp65 protein was significantly decreased by GPE. This indicated that GPE was able to inhibit the nuclear transfer of NF‑κB. The study shows that GPE is able to induce apoptosis and suppress proliferation and metastasis in U2‑OS cells via the inhibition of the nuclear translocation of NF‑κB.

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1.	Longhi A, Errani C, De Paolis M, et al: Primary bone osteosarcoma in the pediatric age: state of the art. Cancer Treat Rev. 32:423–436. 2006. View Article : Google Scholar : PubMed/NCBI
2.	Meyers PA, Schwartz CL, Krailo M, et al: Osteosarcoma: a randomized, prospective trial of the addition of ifosfamide and/or muramyl tripeptide to cisplatin, doxorubicin, and high dose methotrexate. J Clin Oncol. 23:2004–2011. 2005. View Article : Google Scholar : PubMed/NCBI
3.	Jawad MU, Cheung MC, Clarke J, et al: Osteosarcoma: improvement in survival limited to high-grade patients only. J Cancer Res Clin Oncol. 137:597–607. 2011. View Article : Google Scholar : PubMed/NCBI
4.	Rosidah, Yam MF, Sadikun A, et al: Toxicology evaluation of standardized methanol extract of Gynura procumbens. J Ethnopharmacol. 123:244–249. 2009. View Article : Google Scholar : PubMed/NCBI
5.	Nawawi A, Nakamura N, Hattori M, et al: Inhibitory effects of Indonesian medicinal plants on the infection of herpes simplex virus type 1. Phytother Res. 13:37–41. 1999. View Article : Google Scholar : PubMed/NCBI
6.	Iskander MN, Song Y, Coupar IM and Jiratchariyakul W: Antiinf lammatory screening of the medicinal plant Gynura procumbens. Plant Foods Hum Nutr. 57:233–244. 2002. View Article : Google Scholar : PubMed/NCBI
7.	Hoe SZ, Lee CN, Mok SL, et al: Gynura procumbens Merr. decreases blood pressure in rats by vasodilatation via inhibition of calcium channels. Clinics (Sao Paulo). 66:143–150. 2011. View Article : Google Scholar
8.	Kim MJ, Lee HJ, Wiryowidagdo S and Kim HK: Antihypertensive effects of Gynura procumbens extract in spontaneously hypertensive rats. J Med Food. 9:587–590. 2006.
9.	Akowuah GA, Amirin S, Mariam A and Aminah I: Blood sugar lowering activity of Gynura procumbens leaf extracts. J Trop Med Plants. 2:5–10. 2001.
10.	Akowuah GA, Sadikun A and Mariam A: Flavonoid identification and hypoglycaemic studies of butanol fraction from Gynura procumbens. Pharm Biol. 40:405–410. 2002. View Article : Google Scholar
11.	Rosidah, Yam MF, Sadikun A and Asmawi M: Antioxidant potential of Gynura procumbens. Pharm Biol. 46:616–625. 2008. View Article : Google Scholar
12.	Zhang XF and Tan BK: Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and streptozotocin-induced diabetic rats. Singapore Med J. 41:9–13. 2000.
13.	Kim J, Lee CW, Kim EK, et al: Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. J Ethnopharmacol. 137:427–433. 2011.
14.	Davies B, Miles DW, Happerfield LC, et al: Activity of type IV collagenases in benign and malignant breast disease. Br J Cancer. 67:1126–1131. 1993. View Article : Google Scholar : PubMed/NCBI
15.	Davies B, Waxman J, Wasan H, et al: Levels of matrix metalloproteases in bladder cancer correlate with tumour grade and invasion. Cancer Res. 53:5365–5369. 1993.PubMed/NCBI
16.	Iwata H, Kobayashi S, Iwase H, et al: Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas. Jpn J Cancer Res. 87:602–611. 1996. View Article : Google Scholar : PubMed/NCBI
17.	Tokuraku M, Sato H, Murakami S, et al: Activation of the precursor of gelatinase A/72 kDa type IV collagenase/MMP-2 in lung carcinomas correlates with the expression of membrane-type matrix metalloproteinase (MT-MMP) and with lymph node metastasis. Int J Cancer. 64:355–359. 1995. View Article : Google Scholar : PubMed/NCBI
18.	Brown PD, Bloxidge RE, Stuart NS, et al: Association between expression of activated 72-kilodalton gelatinase and tumour spread in non-small-cell lung carcinoma. J Natl Cancer Inst. 85:574–578. 1993. View Article : Google Scholar : PubMed/NCBI
19.	Zhang XX, Fu Z, Zhang Z, et al: Microcystin-LR promotes melanoma cell invasion and enhances matrix metalloproteinase-2/-9 expression mediated by NF-κB activation. Environ Sci Technol. 46:11319–11326. 2012.PubMed/NCBI
20.	Egeblad M and Werb Z: New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2:161–74. 2002. View Article : Google Scholar : PubMed/NCBI
21.	Tsuruda T, Costello-Boerrigter LC and Burnett JC Jr: Matrix metalloproteinases: pathways of induction by bioactive molecules. Heart Fail Rev. 9:53–61. 2004. View Article : Google Scholar : PubMed/NCBI
22.	Andela VB, Gordon AH, Zotalis G, et al: NF-kappaB: a pivotal transcription factor in prostate cancer metastasis to bone. Clin Orthop Relat Res. (415 Suppl): S75–S85. 2003. View Article : Google Scholar : PubMed/NCBI
23.	Felx M, Guyot MC, Isler M, et al: Endothelin-1 (ET-1) promotes MMP-2 and MMP-9 induction involving the transcription factor NF-kappaB in human osteosarcoma. Clin Sci (Lond). 110:645–654. 2006. View Article : Google Scholar : PubMed/NCBI
24.	Werb Z: ECM and cell surface proteolysis: regulating cellular ecology. Cell. 91:439–442. 1997. View Article : Google Scholar : PubMed/NCBI
25.	Finco TS and Baldwin AS: Mechanistic aspects of NF-kappa B regulation: the emerging role of phosporylation and proteolysis. Immunity. 3:263–272. 1995. View Article : Google Scholar : PubMed/NCBI