Differences between brainstem gliomas in juvenile and adult rats

Wang,Yu; Tian,Yongji; Wan,Hong; Li,Dezhi; Wu,Wenhao; Yin,Luxin; Jiang,Jian; Wan,Weiqing; Zhang,Liwei

doi:10.3892/ol.2013.1319

Differences between brainstem gliomas in juvenile and adult rats

Authors:
- Yu Wang
- Yongji Tian
- Hong Wan
- Dezhi Li
- Wenhao Wu
- Luxin Yin
- Jian Jiang
- Weiqing Wan
- Liwei Zhang
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Affiliations: Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China, Beijing Neurosurgical Institute, Beijing 100050, P.R. China
Published online on: April 25, 2013 https://doi.org/10.3892/ol.2013.1319
Pages: 246-250

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Abstract

Clinical studies have shown that gliomas of the brainstem behave differently in children and adults. The aim of the present study was to compare and analyze the differences between these gliomas in juvenile and adult rats with regard to tumor growth, survival, pathology and magnetic resonance imaging (MRI). A total of 25 juvenile and 25 adult Wistar rats were divided into groups A (15 juvenile rats), B (10 juvenile rats), C (15 adult rats) and D (10 adult rats). The rats of groups A and C (experimental) were injected with glioma cells, while groups B and D (control) were injected with a physiological saline solution. Rat neurological signs, survival time, tumor size, hematoxylin and eosin (HE) staining and immunohistochemical staining for MMP-2, MMP-9 and β-catenin were compared. The survival time of group A was 19.47±2.232 days, whereas that of group C was 21.47±2.232 days (P<0.05). The tumor sizes were 4.55 and 4.62 mm (P>0.05) in groups A and C, respectively. HE and immunohistochemical staining revealed no differences between the groups. The results suggest that the growth patterns and invasiveness of brainstem gliomas may vary in children compared with adults due to the varied biological behaviors of the tumor cells.

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