A genetic variation of the p38β promoter region is correlated with an increased risk of sporadic colorectal cancer

Huang,Qinghua; Chen,Dianke; Song,Shunxin; Fu,Xinhui; Wei,Yisheng; Lu,Jiachun; Wang,Lei; Wang,Jianping

doi:10.3892/ol.2013.1334

A genetic variation of the p38β promoter region is correlated with an increased risk of sporadic colorectal cancer

Authors:
- Qinghua Huang
- Dianke Chen
- Shunxin Song
- Xinhui Fu
- Yisheng Wei
- Jiachun Lu
- Lei Wang
- Jianping Wang
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Affiliations: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510655, P.R. China, Gastrointestinal Institute of Sun Yat‑Sen University, The Sixth Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510620, P.R. China, The Institute for Chemical Carcinogenesis, The State Key Lab of Respiratory Disease, Guangzhou Medical College, Guangzhou, Guangdong 510182, P.R. China
Published online on: May 8, 2013 https://doi.org/10.3892/ol.2013.1334
Pages: 3-8

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Abstract

p38 plays a critical role in the proliferation, survival, migration and metastasis of colorectal cancer (CRC) cells. The present study assessed the correlation between a single nucleotide polymorphism (SNP) in the p38β promoter region (rs2235356, ‑1628A>G) and the predisposition of individuals to sporadic CRC in a case‑control study. A genotyping method was developed to detect this SNP, using polymerase chain reaction-restriction fragment length polymorphism (PCR‑RFLP) analysis. A logistic regression analysis was used to determine the odds ratio (OR) and 95% confidence interval (CI). It was revealed that the ‑1628G variant allele was correlated with an increased risk of CRC (OR, 1.99; 95% CI, 1.60‑2.47; P<0.0001). An in silico analysis revealed several transcription factors that either acquired or lost the ability to bind to -1628AA in the p38β promoter region due to the SNP. Therefore, this allelic variant may be a genetic modifier for CRC susceptibility.

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