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Article

Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice

  • Authors:
    • Gendong Yao
    • Chaoqi Liu
    • Hongqi Huo
    • Aimin Liu
    • Bairui Lv
    • Can Zhang
    • Haidong Wang
    • Jinnong Li
    • Lianming Liao
  • View Affiliations / Copyright

    Affiliations: Department of Biological Therapy, Central Hospital of Handan City, Handan, Hebei 056001, P.R. China, Molecular and Biology Institute, Three Gorges University, Yichang, Hubei 443002, P.R. China, Institute of Oncology, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350102, P.R. China
  • Pages: 256-260
    |
    Published online on: May 8, 2013
       https://doi.org/10.3892/ol.2013.1340
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Abstract

Chaenomeles speciosa Nakai is commonly used in traditional Chinese medicine for a variety of health‑promoting effects. The present study aimed to investigate the antitumor effects of Chaenomeles speciosa Nakai. The tumor‑inhibitory activity of the ethanol extract of Chaenomeles speciosa Nakai (EEC) was evaluated by in vitro growth assays of tumor cells and in vivo H22 tumor formation assays in mice. Mitochondrial membrane potential and DNA ladder assays were used to detect tumor cell apoptosis in the presence of EEC. To investigate the cellular targets of EEC, the immunomodulatory genes PD‑L1, Foxp3 and TGF‑β were detected in the tumor tissue using reverse transcription polymerase chain reaction (RT‑PCR). Immune responses were determined by hemolysis and lymphocyte proliferation assays. EEC markedly inhibited the proliferation of the H22 cells in a dose‑dependent manner. Moreover, it induced DNA fragmentation and decreased the mitochondrial membrane potential. In vivo, EEC inhibited tumor growth and enhanced the immune responses in mice, while the expression of PD‑L1, Foxp3 and TGF‑β was inhibited in the tumor tissue. These results provide the first evidence that EEC may inhibit tumor growth by directly killing tumor cells and enhancing immune function. Thus, it is a natural source for safe anticancer medicine.
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Copy and paste a formatted citation
Spandidos Publications style
Yao G, Liu C, Huo H, Liu A, Lv B, Zhang C, Wang H, Li J and Liao L: Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice. Oncol Lett 6: 256-260, 2013.
APA
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C. ... Liao, L. (2013). Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice. Oncology Letters, 6, 256-260. https://doi.org/10.3892/ol.2013.1340
MLA
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C., Wang, H., Li, J., Liao, L."Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice". Oncology Letters 6.1 (2013): 256-260.
Chicago
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C., Wang, H., Li, J., Liao, L."Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice". Oncology Letters 6, no. 1 (2013): 256-260. https://doi.org/10.3892/ol.2013.1340
Copy and paste a formatted citation
x
Spandidos Publications style
Yao G, Liu C, Huo H, Liu A, Lv B, Zhang C, Wang H, Li J and Liao L: Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice. Oncol Lett 6: 256-260, 2013.
APA
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C. ... Liao, L. (2013). Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice. Oncology Letters, 6, 256-260. https://doi.org/10.3892/ol.2013.1340
MLA
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C., Wang, H., Li, J., Liao, L."Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice". Oncology Letters 6.1 (2013): 256-260.
Chicago
Yao, G., Liu, C., Huo, H., Liu, A., Lv, B., Zhang, C., Wang, H., Li, J., Liao, L."Ethanol extract of Chaenomeles speciosa Nakai induces apoptosis in cancer cells and suppresses tumor growth in mice". Oncology Letters 6, no. 1 (2013): 256-260. https://doi.org/10.3892/ol.2013.1340
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