Small compound 6‑O‑angeloylplenolin induces caspase‑dependent apoptosis in human multiple myeloma cells

Liu,Ying; Dong,Ying; Zhang,Bo; Cheng,Yong‑Xian

doi:10.3892/ol.2013.1370

August 2013 Volume 6 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August 2013 Volume 6 Issue 2

Full Size Image

Article

Small compound 6‑O‑angeloylplenolin induces caspase‑dependent apoptosis in human multiple myeloma cells

Authors:
- Ying Liu
- Ying Dong
- Bo Zhang
- Yong‑Xian Cheng
View Affiliations / Copyright

Affiliations: The Shenzhen Key Lab of Gene and Antibody Therapy, Center for Biotech and BioMedicine and Division of Life Sciences, Shenzhen Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China, Department of Oncology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China, State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650204, P.R. China
Pages: 556-558
|
Published online on: May 30, 2013

https://doi.org/10.3892/ol.2013.1370
Expand metrics +

Abstract

6‑O‑angeloylplenolin (6‑OAP) is a sesquiterpene lactone agent that has been previously demonstrated to inhibit the growth of multiple myeloma (MM) cells through mitotic arrest with accumulated cyclin B1. In the present study, the levels of apoptosis were analyzed in dexamethasone‑sensitive (MM.1S), dexamethasone‑resistant (U266) and chemotherapy‑sensitive (RPMI 8226) myeloma cell lines. Enhanced apoptosis was identified following a 48‑h incubation with 6‑OAP (0‑10 µM) that induced a dose‑dependent decrease in pro‑casp‑3 and the cleavage of its substrate, anti‑poly (ADP-ribose) polymerase (PARP). In addition, time-dependent cleavage of PARP was also detected in U266 and MM.1S cells. The mechanism of 6‑OAP cytotoxicity in all cell lines was associated with the induction of apoptosis with the presence of cleaved caspase‑3 and PARP. In conclusion, 6‑OAP‑induced apoptosis is caspase‑dependent. These observations are likely to provide a framework for future studies of 6‑OAP therapy in MM.

View Figures

View References

1.	Chauhan D and Anderson KC: Apoptosis in multiple myeloma: therapeutic implications. Apoptosis. 6:47–55. 2001. View Article : Google Scholar : PubMed/NCBI
2.	Changlong L, Hezhen W, Yongping H, Yanfang Y, Yanwen L and Jianwen L: 6-O-Angeloylenolin induces apoptosis through a mitochondrial/caspase and NF-kappaB pathway in human leukemia HL60 cells. Biomed Pharmacother. 62:401–409. 2008. View Article : Google Scholar : PubMed/NCBI
3.	Su M, Chung HY and Li Y: 6-O-Angeloylenolin induced cell-cycle arrest and apoptosis in human nasopharyngeal cancer cells. Chem Biol Interact. 189:167–176. 2011. View Article : Google Scholar : PubMed/NCBI
4.	Ding LF, Liu Y, Liang HX, Liu DP, Zhou GB and Cheng YX: Two new terpene glucosides and antitumor agents from Centipeda minima. J Asian Nat Prod Res. 11:732–736. 2009. View Article : Google Scholar : PubMed/NCBI
5.	Liu Y, Chen XQ, Liang HX, Zhang FX, Zhang B, Jin J, Chen YL, Cheng YX and Zhou GB: Small compound 6-o-angeloylplenolin induces mitotic arrest and exhibits therapeutic potentials in multiple myeloma. PLoS One. 6:e219302011. View Article : Google Scholar : PubMed/NCBI
6.	Hideshima T, Richardson P, Chauhan D, Palombella VJ, Elliott PJ, Adams J and Anderson KC: The proteasome inhibitor PS-341 inhibits growth, induces apoptosis and overcomes drug resistance in human multiple myeloma cells. Cancer Res. 61:3071–3076. 2001.PubMed/NCBI
7.	Taylor RS and Towers GH: Antibacterial constituents of the Nepalese medicinal herb, Centipeda minima. Phytochemistry. 47:631–634. 1998. View Article : Google Scholar : PubMed/NCBI
8.	Francois G, Passreiter CM, Woerdenbag HJ and Van LM: Antiplasmodial activities and cytotoxic effects of aqueous extracts and sesquiterpene lactones from Neurolaena lobata. Planta Med. 62:126–129. 1996. View Article : Google Scholar : PubMed/NCBI
9.	Schwikkard S and van Heerden FR: Antimalarial activity of plant metabolites. Nat Prod Rep. 19:675–692. 2002.PubMed/NCBI
10.	Nicholson DW: Caspase structure, proteolytic substrates and function during apoptotic cell death. Cell Death Differ. 6:1028–1042. 1999. View Article : Google Scholar : PubMed/NCBI
11.	Johnstone RW, Ruefli AA and Lowe SW: Apoptosis: a link between cancer genetics and chemotherapy. Cell. 108:153–164. 2002. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Small compound 6‑O‑angeloylplenolin induces caspase‑dependent apoptosis in human multiple myeloma cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles