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Article

Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells

  • Authors:
    • Zhi-Min Li
    • Yu-Wei Pu
    • Bao-Song Zhu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Wujiang Affiliated Hospital, Nantong University, Wujiang, Jiangsu 215200, P.R. China, Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China
  • Pages: 432-436
    |
    Published online on: June 11, 2013
       https://doi.org/10.3892/ol.2013.1390
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Abstract

The aim of the present study was to investigate the effect of the nuclear factor-κB (NF-κB) p65 inhibitor, SN50, on the invasiveness and mechanisms of SGC7901 human gastric carcinoma cell xenografts in nude mice. Nude mice were randomly divided into model control and SN50 treatment groups. On days 5, 10 and 15 following treatment, the tumor samples were observed and a selection of parameters were recorded, including the level of tumor growth inhibition, the pathological changes in the tumor specimens, the expression levels of matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), tissue inhibitor of metalloproteinases type-1 (TIMP-1) and vascular endothelial growth factor (VEGF) and the apoptosis indices in the tumor samples. The results demonstrated that treating the tumor with SN50 for 5, 10 and 15 days inhibited carcinoma growth in comparison with the control group. Hematoxylin and eosin (HE) staining indicated that the level of inhibition increased progressively, in correlation with apoptosis. The expression of the MMP-9, PCNA and VEGF proteins was observed to be downregulated, while that of the TIMP-1 protein was shown to be upregulated, using immunohistochemical staining. In conclusion, the NF-κB p65 inhibitor, SN50, inhibited the invasiveness of the gastric cancer cells by downregulating the protein expression of MMP-9, PCNA and VEGF and upregulating the protein expression of TIMP-1. It was further suggested that SN50 may be a molecular target of anti-invasion therapy for gastric cancer, and that the inhibition of the NF-κB p65 signaling pathway may be considered as a potential strategy for treating gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Pu Y and Zhu B: Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells. Oncol Lett 6: 432-436, 2013.
APA
Li, Z., Pu, Y., & Zhu, B. (2013). Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells. Oncology Letters, 6, 432-436. https://doi.org/10.3892/ol.2013.1390
MLA
Li, Z., Pu, Y., Zhu, B."Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells". Oncology Letters 6.2 (2013): 432-436.
Chicago
Li, Z., Pu, Y., Zhu, B."Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells". Oncology Letters 6, no. 2 (2013): 432-436. https://doi.org/10.3892/ol.2013.1390
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Pu Y and Zhu B: Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells. Oncol Lett 6: 432-436, 2013.
APA
Li, Z., Pu, Y., & Zhu, B. (2013). Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells. Oncology Letters, 6, 432-436. https://doi.org/10.3892/ol.2013.1390
MLA
Li, Z., Pu, Y., Zhu, B."Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells". Oncology Letters 6.2 (2013): 432-436.
Chicago
Li, Z., Pu, Y., Zhu, B."Blockade of NF-κB nuclear translocation results in the inhibition of the invasiveness of human gastric cancer cells". Oncology Letters 6, no. 2 (2013): 432-436. https://doi.org/10.3892/ol.2013.1390
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