Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF‑κB signaling pathway

Shao,Hongmin; Yang,Baowen; Hu,Rongrong; Wang,Yingying

doi:10.3892/ol.2013.1399

Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF‑κB signaling pathway

Authors:
- Hongmin Shao
- Baowen Yang
- Rongrong Hu
- Yingying Wang
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Affiliations: Department of Oncology, Hospital of Traditional Chinese Medicine, Yantai, Shandong 264000, P.R. China
Published online on: June 14, 2013 https://doi.org/10.3892/ol.2013.1399
Pages: 517-520

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Abstract

Matrine is an alkaloid isolated from Sophora flavescens. The present study aimed to determine whether matrine effectively inhibits the proliferation of breast cancer cells, and the underlying mechanism(s) of its antitumor function. The effects of matrine on the cell viability of ER‑positive MCF7 cells, HER2‑positive BT‑474 cells and highly metastatic MDA‑MB‑231 cells were measured using MTT and apoptosis assays. Western blot analysis was performed to investigate the expression levels of the inhibitor of κB (IκB) kinase β (IKKβ) in cells treated with or without matrine. It was observed that the matrine treatment resulted in the death of the three types of cancer cells, but significantly less toxicity was observed in the control cancer cells. The experimental results also suggested that the antitumor effects of matrine on breast cancer cells may be associated with the downregulation of IKKβ expression by matrine, as indicated by the western blot analysis results. The present results suggested that matrine may be used as an effective drug candidate for treating breast cancers in the future, following further research.

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