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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September 2013 Volume 6 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells

  • Authors:
    • Zhongbin Xie
    • Liping Cao
    • Jun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Medicine, Yulin Number Two Hospital, Yulin 719000, P.R. China, Department of Obstetrics and Gynecology, Yulin Traditional Chinese Medical Hospital, Yulin 719000, P.R. China, Department of Clinical Laboratory Medicine, 3201 Hospital, Hanzhong, Shanxi 723000, P.R. China
  • Pages: 795-800
    |
    Published online on: June 28, 2013
       https://doi.org/10.3892/ol.2013.1432
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Abstract

Drug resistance is a major problem encountered in the treatment of ovarian cancer. Previous studies have demonstrated that in several types of cancer the overexpression of the multidrug resistance 1 (MDR1) gene is mainly associated with drug resistance. The present study aimed to investigate the role of miR‑21 in the development of drug resistance in ovarian cancer cells. The expression levels of miR‑21 in the ovarian cancer A2780 and A2780/taxol cell lines were detected by stem‑loop real‑time PCR. A2780 and A2780/taxol cells were transfected with mimics or inhibitors of miR‑21 or negative control RNA. The expression levels of P‑glycoprotein (P‑gp) and hypoxia‑inducible factor‑1α (HIF‑1α) proteins were assessed by western blot analysis. Drug sensitivity was analyzed by the 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The expression levels of miR‑21 and P‑gp were upregulated to a greater extent in the paclitaxel‑resistant ovarian cancer A2780/taxol cell line compared with the parental A2780 cell line. Transfection of A2780/taxol cells with inhibitors of miR‑21 decreased the expression levels of the P‑gp and HIF‑1α proteins, and increased the sensitivity of the A2780/taxol cells to paclitaxel. The expression levels of P‑gp were additionally increased; however, the sensitivity of the miR‑21 mimic‑treated A2780 cells to paclitaxel was decreased. miR‑21 may be involved in the development of drug resistance and the regulation of MDR1/P‑gp expression, at least in part, by targeting HIF‑1α in ovarian cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xie Z, Cao L and Zhang J: miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells. Oncol Lett 6: 795-800, 2013.
APA
Xie, Z., Cao, L., & Zhang, J. (2013). miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells. Oncology Letters, 6, 795-800. https://doi.org/10.3892/ol.2013.1432
MLA
Xie, Z., Cao, L., Zhang, J."miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells". Oncology Letters 6.3 (2013): 795-800.
Chicago
Xie, Z., Cao, L., Zhang, J."miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells". Oncology Letters 6, no. 3 (2013): 795-800. https://doi.org/10.3892/ol.2013.1432
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Z, Cao L and Zhang J: miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells. Oncol Lett 6: 795-800, 2013.
APA
Xie, Z., Cao, L., & Zhang, J. (2013). miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells. Oncology Letters, 6, 795-800. https://doi.org/10.3892/ol.2013.1432
MLA
Xie, Z., Cao, L., Zhang, J."miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells". Oncology Letters 6.3 (2013): 795-800.
Chicago
Xie, Z., Cao, L., Zhang, J."miR‑21 modulates paclitaxel sensitivity and hypoxia‑inducible factor‑1α expression in human ovarian cancer cells". Oncology Letters 6, no. 3 (2013): 795-800. https://doi.org/10.3892/ol.2013.1432
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