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Article

Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice

  • Authors:
    • Lei He
    • Han-Xian Gong
    • Xiang-Pen Li
    • Yi-Dong Wang
    • Yi Li
    • Jun-Jian Huang
    • Dan Xie
    • Hsiang-Fu Kung
    • Ying Peng
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. China, Laboratory of Tumor and Molecular Biology, Beijing Institute of Biotechnology, Beijing 100071, P.R. China, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. China, Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, P.R. China
  • Pages: 748-752
    |
    Published online on: July 16, 2013
       https://doi.org/10.3892/ol.2013.1470
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Abstract

A 27‑kDa C‑terminal fragment of human telomerase reverse transcriptase, hTERTC27, has previously been reported to inhibit the growth and tumorigenicity of HeLa human cervical cancer cells and U87‑MG human glioblastoma multiforme cells. However, the antitumor effects of hTERTC27 in hepatoma and its underlying mechanisms are unclear. In the current study, the therapeutic effect of hTERTC27, mediated by recombinant adenovirus, in hepatocellular carcinoma (HCC) was explored in vitro and in vivo to investigate the possible mechanisms. The results indicated that recombinant adenovirus carrying hTERTC27 (rAdv‑hTERTC27) effectively inhibited the growth and induced apoptosis of the Hepa 1‑6 HCC cells. Dendritic cells transduced with rAdv‑hTERTC27 were highly effective at inducing antigen‑specific T cell proliferation and increasing the activated cytotoxicity of T cells against Hepa 1‑6 cells. HCC was inhibited significantly when a single dose of 5x107 pfu rAdv‑hTERTC27 was administered intravenously. In summary, the results of this study demonstrated that rAdv‑hTERTC27 may serve as a reagent for intravenous administration when combined with telomerase‑based gene therapy and immunotherapy for cancer.
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Copy and paste a formatted citation
Spandidos Publications style
He L, Gong H, Li X, Wang Y, Li Y, Huang J, Xie D, Kung H and Peng Y: Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice. Oncol Lett 6: 748-752, 2013.
APA
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J. ... Peng, Y. (2013). Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice. Oncology Letters, 6, 748-752. https://doi.org/10.3892/ol.2013.1470
MLA
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J., Xie, D., Kung, H., Peng, Y."Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice". Oncology Letters 6.3 (2013): 748-752.
Chicago
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J., Xie, D., Kung, H., Peng, Y."Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice". Oncology Letters 6, no. 3 (2013): 748-752. https://doi.org/10.3892/ol.2013.1470
Copy and paste a formatted citation
x
Spandidos Publications style
He L, Gong H, Li X, Wang Y, Li Y, Huang J, Xie D, Kung H and Peng Y: Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice. Oncol Lett 6: 748-752, 2013.
APA
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J. ... Peng, Y. (2013). Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice. Oncology Letters, 6, 748-752. https://doi.org/10.3892/ol.2013.1470
MLA
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J., Xie, D., Kung, H., Peng, Y."Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice". Oncology Letters 6.3 (2013): 748-752.
Chicago
He, L., Gong, H., Li, X., Wang, Y., Li, Y., Huang, J., Xie, D., Kung, H., Peng, Y."Inhibition of hepatocellular carcinoma growth by adenovirus‑mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice". Oncology Letters 6, no. 3 (2013): 748-752. https://doi.org/10.3892/ol.2013.1470
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