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October 2013 Volume 6 Issue 4

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2

  • Authors:
    • Jia‑Xuan Xin
    • Zhen Yue
    • Shuai Zhang
    • Zhong‑Hua Jiang
    • Ping‑Yu Wang
    • You‑Jie Li
    • Min Pang
    • Shu‑Yang Xie
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Tumour Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Yantai City Hospital for Infectious Disease, Yantai, Shandong 264000, P.R. China, Yantai Traditional Chinese Medical‑Science (TCM) Hospital, Yantai, Shandong 264000, P.R. China
  • Pages: 1025-1030
    |
    Published online on: July 17, 2013
       https://doi.org/10.3892/ol.2013.1473
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Abstract

MicroRNAs (miRNAs) have significant roles in cell processes, including proliferation, apoptosis and stress responses. To investigate the involvement of miR‑99 in the inhibition of HeLa cell proliferation, an miR‑99 gene expression vector (pU6.1/miR‑99), which overexpressed miR‑99 in HeLa cells after transient transfection, was constructed. The expression of miR‑99 was detected by qPCR. Cell proliferation and apoptosis were analyzed by cell viability, proliferation and apoptosis assays, as well as by electron microscopy. The results showed that overexpression of miR‑99 in HeLa cells increased the HeLa cell mortality rate. Moreover, miR‑99 overexpression was able to markedly inhibit HeLa cell proliferation according to the 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. The cell apoptosis rate was significantly higher in pU6.1/miR‑99‑treated cells compared with that in the control cultures. Increases in intracellular electron density, as well as the proportion of nuclear plasma, blebbing phenomena and apoptotic bodies were observed in pU6.1/miR‑99‑treated cells compared with control cultures according to electron microscopy analysis. The Tribbles 2 (TRIB2) 3'‑untranslated region was also observed to be targeted by miR‑99 and the results further demonstrated that miR‑99 was able to negatively regulate TRIB2 expression in HeLa cells The results indicate that miR‑99 acts as a tumor suppressor gene in HeLa cells, establishing a theoretical basis for its application in cancer therapeutics.
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Copy and paste a formatted citation
Spandidos Publications style
Xin JX, Yue Z, Zhang S, Jiang ZH, Wang PY, Li YJ, Pang M and Xie SY: miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2. Oncol Lett 6: 1025-1030, 2013.
APA
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y. ... Xie, S. (2013). miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2. Oncology Letters, 6, 1025-1030. https://doi.org/10.3892/ol.2013.1473
MLA
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y., Pang, M., Xie, S."miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2". Oncology Letters 6.4 (2013): 1025-1030.
Chicago
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y., Pang, M., Xie, S."miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2". Oncology Letters 6, no. 4 (2013): 1025-1030. https://doi.org/10.3892/ol.2013.1473
Copy and paste a formatted citation
x
Spandidos Publications style
Xin JX, Yue Z, Zhang S, Jiang ZH, Wang PY, Li YJ, Pang M and Xie SY: miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2. Oncol Lett 6: 1025-1030, 2013.
APA
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y. ... Xie, S. (2013). miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2. Oncology Letters, 6, 1025-1030. https://doi.org/10.3892/ol.2013.1473
MLA
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y., Pang, M., Xie, S."miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2". Oncology Letters 6.4 (2013): 1025-1030.
Chicago
Xin, J., Yue, Z., Zhang, S., Jiang, Z., Wang, P., Li, Y., Pang, M., Xie, S."miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2". Oncology Letters 6, no. 4 (2013): 1025-1030. https://doi.org/10.3892/ol.2013.1473
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