miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2

Xin,Jia‑Xuan; Yue,Zhen; Zhang,Shuai; Jiang,Zhong‑Hua; Wang,Ping‑Yu; Li,You‑Jie; Pang,Min; Xie,Shu‑Yang

doi:10.3892/ol.2013.1473

miR‑99 inhibits cervical carcinoma cell proliferation by targeting TRIB2

Authors:
- Jia‑Xuan Xin
- Zhen Yue
- Shuai Zhang
- Zhong‑Hua Jiang
- Ping‑Yu Wang
- You‑Jie Li
- Min Pang
- Shu‑Yang Xie
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Affiliations: Key Laboratory of Tumour Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Yantai City Hospital for Infectious Disease, Yantai, Shandong 264000, P.R. China, Yantai Traditional Chinese Medical‑Science (TCM) Hospital, Yantai, Shandong 264000, P.R. China
Published online on: July 17, 2013 https://doi.org/10.3892/ol.2013.1473
Pages: 1025-1030

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Abstract

MicroRNAs (miRNAs) have significant roles in cell processes, including proliferation, apoptosis and stress responses. To investigate the involvement of miR‑99 in the inhibition of HeLa cell proliferation, an miR‑99 gene expression vector (pU6.1/miR‑99), which overexpressed miR‑99 in HeLa cells after transient transfection, was constructed. The expression of miR‑99 was detected by qPCR. Cell proliferation and apoptosis were analyzed by cell viability, proliferation and apoptosis assays, as well as by electron microscopy. The results showed that overexpression of miR‑99 in HeLa cells increased the HeLa cell mortality rate. Moreover, miR‑99 overexpression was able to markedly inhibit HeLa cell proliferation according to the 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. The cell apoptosis rate was significantly higher in pU6.1/miR‑99‑treated cells compared with that in the control cultures. Increases in intracellular electron density, as well as the proportion of nuclear plasma, blebbing phenomena and apoptotic bodies were observed in pU6.1/miR‑99‑treated cells compared with control cultures according to electron microscopy analysis. The Tribbles 2 (TRIB2) 3'‑untranslated region was also observed to be targeted by miR‑99 and the results further demonstrated that miR‑99 was able to negatively regulate TRIB2 expression in HeLa cells The results indicate that miR‑99 acts as a tumor suppressor gene in HeLa cells, establishing a theoretical basis for its application in cancer therapeutics.

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