Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer

Chaleshi,Vahid; Haghighi,Mahdi   Montazer; Savabkar,Sanaz; Zali,Neda; Vahedi,Mohsen; Khanyaghma,Mahsa; Javadi,Gholam  Reza; Asadzade,Hamid; Zali,Mohammad  Reza

doi:10.3892/ol.2013.1481

Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer

Authors:
- Vahid Chaleshi
- Mahdi Montazer Haghighi
- Sanaz Savabkar
- Neda Zali
- Mohsen Vahedi
- Mahsa Khanyaghma
- Gholam Reza Javadi
- Hamid Asadzade
- Mohammad Reza Zali
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Affiliations: Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Published online on: July 22, 2013 https://doi.org/10.3892/ol.2013.1481
Pages: 1079-1083

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Abstract

Colorectal cancer (CRC) is an important disorder that results from genetic and epigenetic alterations in one colonic epithelial cell. Epidermal growth factor (EGF) is critical in the development of tumors in epithelial tissues. Variations in the DNA sequence of the EGF gene may be particularly significant with regard to susceptibility to CRC. The present study aimed to investigate the effect of the EGF gene single nucleotide polymorphism (SNP), rs2298979, on CRC. In this prospective study, 220 samples were collected from patients with CRC and compared with 220 matched healthy controls. Genotyping was performed using the polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP) method, and the result was validated by direct sequencing. A significant correlation was observed between the rs2298979 variant in the EGF gene and CRC. The frequency of the A/G genotype in the control group was higher than in the patients with sporadic CRC [odds ratio (OR), 0.488; 95% confidence interval (CI), 0.307‑0.774; P=0.002]. In this study there were no individuals with a G/G genotype. Although the frequency of the G and A alleles was similar in the healthy control and CRC patient groups, individuals with the A/G genotype were less susceptible to CRC compared with those with the A/A genotype.

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1	Janakiram NB and Rao CV: Molecular markers and targets for colorectal cancer prevention. Acta Pharmacol Sin. 29:1–20. 2008. View Article : Google Scholar : PubMed/NCBI
2	Haggar FA and Boushey RP: Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clin Colon Rectal Surg. 22:191–197. 2009. View Article : Google Scholar : PubMed/NCBI
3	Chan AO, Soliman AS, Zhang Q, et al: Differing DNA methylation patterns and gene mutation frequencies in colorectal carcinomas from Middle Eastern countries. Clin Cancer Res. 11:8281–8287. 2005. View Article : Google Scholar : PubMed/NCBI
4	Yee YK, Tan VP, Chan P, Hung IF, Pang R and Wong BC: Epidemiology of colorectal cancer in Asia. J Gastroenterol Hepatol. 24:1810–1816. 2009. View Article : Google Scholar : PubMed/NCBI
5	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar
6	Cheung DY, Kim TH, Kim CW, et al: The anatomical distribution of colorectal cancer in Korea: evaluation of the incidence of proximal and distal lesions and synchronous adenomas. Intern Med. 47:1649–1654. 2008. View Article : Google Scholar : PubMed/NCBI
7	Islamic Republic of Iran Ministry of Health and Medical Education, Office of Deputy Minister for Health Center for Disease Control, Cancer Office. Iranian Annual National Cancer Registration Report. 2005–2006. Mar;2007.
8	Fahy B and Bold RJ: Epidemiology and molecular genetics of colorectal cancer. Surg Oncol. 7:115–123. 1998. View Article : Google Scholar : PubMed/NCBI
9	Lin L, Li G, Zhang Z, et al: Association of epidermal growth factor +61 A/G polymorphism in Chinese patients with colon cancer. Genet Test Mol Biomarkers. 16:1142–1145. 2012.
10	Wilson KJ, Gilmore JL, Foley J, Lemmon MA and Riese DJ II: Functional selectivity of EGF family peptide growth factors: implications for cancer. Pharmacol Ther. 122:1–8. 2009. View Article : Google Scholar : PubMed/NCBI
11	Henson ES and Gibson SB: Surviving cell death through epidermal growth factor (EGF) signal transduction pathways: implications for cancer therapy. Cell Signal. 18:2089–2097. 2006. View Article : Google Scholar : PubMed/NCBI
12	Li TF, Ren KW and Liu PF: Meta-analysis of epidermal growth factor polymorphisms and cancer risk: involving 9,779 cases and 15,932 controls. DNA Cell Biol. 31:568–574. 2011.PubMed/NCBI
13	Normanno N, De Luca A, Bianco C, et al: Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 366:2–16. 2006. View Article : Google Scholar : PubMed/NCBI
14	Li LC, Chui RM, Sasaki M, et al: A single nucleotide polymorphism in the E-cadherin gene promoter alters transcriptional activities. Cancer Res. 60:873–876. 2000.PubMed/NCBI
15	Zhang Y-M, Cao C and Liang K: Genetic polymorphism of epidermal growth factor 61A>G and cancer risk: a meta-analysis. Cancer Epidemiol. 34:150–156. 2010. View Article : Google Scholar
16	Xu W, Li Y, Wang X, et al: Association between EGF promoter polymorphisms and cancer risk: a meta-analysis. Med Oncol. 27:1389–1397. 2010. View Article : Google Scholar : PubMed/NCBI
17	Tan D, Xu J, Li Y and Lai R: Association between +61G polymorphism of the EGF gene and glioma risk in different ethnicities: a meta-analysis. Tohoku J Exp Med. 222:229–235. 2010.
18	Miller SA, Dykes DD and Polesky HF: A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:12151988. View Article : Google Scholar : PubMed/NCBI
19	Kang HG, Choi JE, Lee WK, et al: +61A>G polymorphism in the EGF gene does not increase the risk of lung cancer. Respirology. 12:902–905. 2007.
20	Spindler KL, Nielsen JN, Ornskov D, Brandslund I and Jakobsen A: Epidermal growth factor (EGF) A61G polymorphism and EGF gene expression in normal colon tissue from patients with colorectal cancer. Acta Oncol. 46:1113–1117. 2007. View Article : Google Scholar : PubMed/NCBI
21	Werner T: Functional in silico analysis of non-coding SNPs. Bioinformatics for Geneticists. Barnes MR and Gray IC: John Wiley and Sons, Ltd; Chichester: pp. 273–287. 2003
22	Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar
23	Millar DS, Horan M, Chuzhanova NA and Cooper DN: Characterisation of a functional intronic polymorphism in the human growth hormone (GH1) gene. Hum Genomics. 4:289–301. 2010. View Article : Google Scholar : PubMed/NCBI
24	Li L, Yun JH, Lee JH, Song S, Choi BC and Baek KH: Association study of +45G15G(T/G) and +276(G/T) polymorphisms in the adiponectin gene in patients with polycystic ovary syndrome. Int J Mol Med. 27:283–287. 2011.