A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant

  • Authors:
    • Liran Cui
    • Yongxu Sun
    • Hao Xu
    • Huiyu Xu
    • Huan Cong
    • Jicheng Liu
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  • Published online on: July 23, 2013     https://doi.org/10.3892/ol.2013.1484
  • Pages: 1039-1044
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Abstract

In the present study, a low molecular weight polysaccharide, ABP‑AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP‑AW1 is considered to create a ‘depot’ of antigen at a subcutaneous injection site. ICR mice were immunized with 100 µg ovalbumin (OVA) alone or with 100 µg OVA formulated in 0.9% saline containing 200 µg aluminum (alum) or ABP‑AW1 (50, 100 and 200 µg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA‑specific antibody levels in the serum were determined. The OVA/ABP‑AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen‑specific CD4+ T cell activation, as determined by splenic CD4+CD69+ T cells and Th1 cytokine interferon (IFN)‑γ release. The combination of ABP‑AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP‑AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP‑AW1 stimulated Th1‑type immunity. We suggest that ABP‑AW1 may serve as a new adjuvant.
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October 2013
Volume 6 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cui L, Sun Y, Xu H, Xu H, Cong H and Liu J: A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncol Lett 6: 1039-1044, 2013
APA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., & Liu, J. (2013). A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncology Letters, 6, 1039-1044. https://doi.org/10.3892/ol.2013.1484
MLA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6.4 (2013): 1039-1044.
Chicago
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6, no. 4 (2013): 1039-1044. https://doi.org/10.3892/ol.2013.1484