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Article

A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant

  • Authors:
    • Liran Cui
    • Yongxu Sun
    • Hao Xu
    • Huiyu Xu
    • Huan Cong
    • Jicheng Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medicine Research, The First Affliated Hospital, Qiqihar Medical University, Qiqihar, Heilongjiang 161041, P.R. China, Department of Medicinal Chemistry and Biomacromolecules, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China
  • Pages: 1039-1044
    |
    Published online on: July 23, 2013
       https://doi.org/10.3892/ol.2013.1484
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Abstract

In the present study, a low molecular weight polysaccharide, ABP‑AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP‑AW1 is considered to create a ‘depot’ of antigen at a subcutaneous injection site. ICR mice were immunized with 100 µg ovalbumin (OVA) alone or with 100 µg OVA formulated in 0.9% saline containing 200 µg aluminum (alum) or ABP‑AW1 (50, 100 and 200 µg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA‑specific antibody levels in the serum were determined. The OVA/ABP‑AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen‑specific CD4+ T cell activation, as determined by splenic CD4+CD69+ T cells and Th1 cytokine interferon (IFN)‑γ release. The combination of ABP‑AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP‑AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP‑AW1 stimulated Th1‑type immunity. We suggest that ABP‑AW1 may serve as a new adjuvant.
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1 

Kubena KS and McMurray DN: Nutrition and the immune system: a review of nutrient-nutrient interactions. J Am Diet Assoc. 96:1156–1164. 1996. View Article : Google Scholar : PubMed/NCBI

2 

Marrack P, McKee AS and Munks MW: Towards an understanding of the adjuvant action of aluminium. Nat Rev Immunol. 9:287–293. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Singh M, Chakrapani A and O’Hagan D: Nanoparticles and microparticles as vaccine-delivery systems. Expert Rev Vaccines. 6:797–808. 2007. View Article : Google Scholar : PubMed/NCBI

4 

HogenEsch H: Mechanisms of stimulation of the immune response by aluminum adjuvants. Vaccine. 20(Suppl 3): S34–S39. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Schirmbeck R, Melber K, Kuhröber A, Janowicz ZA and Reimann J: Immunization with soluble hepatitis B virus surface protein elicits murine H-2 class I-restricted CD8+ cytotoxic T lymphocyte responses in vivo. J Immunol. 152:1110–1119. 1994.PubMed/NCBI

6 

Aguilar JC and Rodríguez EG: Vaccine adjuvants revisited. Vaccine. 25:3752–3762. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Guy B: The perfect mix: recent progress in adjuvant research. Nat Rev Microbiol. 5:505–517. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Pascual DM, Morales RD, Gil ED, Muñoz LM, López JE and Casanueva OL: Adjuvants: Present regulatory challenges. Vaccine. 24(Suppl 2): S88–S89. 2006. View Article : Google Scholar

9 

Petrovsky N and Cooper PD: Carbohydrate-based immune adjuvants. Expert Rev Vaccines. 10:523–537. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Rivas E, Gómez-Arnáiz M, Ricoy JR, et al: Macrophagic myofasciitis in childhood: a controversial entity. Pediatr Neurol. 33:350–356. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Gherardi RK, Coquet M, Cherin P, et al: Macrophagic myofasciitis lesions assess long-term persistence of vaccine-derived aluminium hydroxide in muscle. Brain. 124:1821–1831. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Ramberg JE, Nelson ED and Sinnott RA: Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr J. 9:542010. View Article : Google Scholar : PubMed/NCBI

13 

Paulsen BS: Plant polysaccharides with immunostimulatory activities. Curr Org Chem. 5:939–950. 2001. View Article : Google Scholar

14 

Kusaykin M, Bakunina I, Sova V, et al: Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds. Biotechnol J. 3:904–915. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Paulsen BS: Biologically active polysaccharides as possible lead compounds. Phytochem Rev. 1:379–387. 2002. View Article : Google Scholar

16 

Bacon A, Makin J, Sizer PJ, et al: Carbohydrate biopolymers enhance antibody responses to mucosally delivered vaccine antigens. Infect Immun. 68:5764–5770. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Stambas J, Pietersz G, McKenzie I and Cheers C: Oxidised mannan as a novel adjuvant inducing mucosal IgA production. Vaccine. 20:1068–1078. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Mond JJ, Vos Q, Lees A and Snapper CM: T cell independent antigens. Curr Opin Immunol. 7:349–354. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Hetland G, Johnson E, Lyberg T, Bernardshaw S, Tryggestad AM and Grinde B: Effects of the medicinal mushroom Agaricus blazei Murill on immunity, infection and cancer. Scand J Immunol. 68:363–370. 2008.

20 

Endo M, Beppu H, Akiyama H, et al: Agaritine purified from Agaricus blazei Murill exerts anti-tumor activity against leukemic cells. Biochim Biophy Acta. 1800:669–673. 2010.

21 

Takaku T, Kimura Y and Okuda H: Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. J Nutr. 131:1409–1413. 2001.PubMed/NCBI

22 

Oh TW, Kim YA, Jang WJ, Byeon JI, Ryu CH, Kim JO and Ha YL: Semipurified fractions from the submerged-culture broth of Agaricus blazei Murill reduce blood glucose levels in streptozotocin-induced diabetic rats. J Agr Food Chem. 58:4113–4119. 2010.PubMed/NCBI

23 

Niu YC, Liu JC, Zhao XM and Cao J: A low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) exhibits its anti-metastatic effect by down-regulating metalloproteinase-9 and up-regulating Nm23-H1. Am J Chin Med. 37:909–921. 2009.PubMed/NCBI

24 

Liu JC, Yue LL, Zhang C, et al: A polysaccharide isolated from Agaricus blazei Murill inhibits sialyl Lewis X/E-selectin-mediated metastatic potential in HT-29 cells through downregulating α-1,3-fucosyltransferase-VII (FucT-VII). Carbohydr Polym. 79:921–926. 2010.

25 

Yue LL, Cui HX, Li CC, Lin Y, Niu YC, Sun YX, Niu YC, Wen XC and Liu JC: A polysaccharide from Agaricus blazei attenuates tumor cell adhesion via inhibiting E-selectin expression. Carbohydr Polym. 88:1326–1333. 2012.

26 

Liu JC and Sun YX: Structural analysis of an alkali-extractable and water-soluble polysaccharide (ABP-AW1) from the fruiting bodies of Agaricus blazei Murill. Carbohydr Polym. 86:429–432. 2011. View Article : Google Scholar

27 

Sun Y, Wang S, Li T, Li X, Jiao L and Zhang L: Purification, structure and immunobiological activity of a new water-soluble polysaccharide from the mycelium of Polyporus albicans (Imaz.) Teng. Bioresour Technol. 99:900–904. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Concha C, Hu S and Holmberg O: The proliferative responses of cow stripping milk and blood lymphocytes to pokeweed mitogen and ginseng in vitro. Vet Res. 27:107–115. 1996.PubMed/NCBI

29 

Sjölander A, van’t Land B and Lövgren Bengtsson K: Iscoms containing purified Quillaja saponins upregulate both Th1-like and Th2-like immune responses. Cell Immunol. 177:69–76. 1997.

30 

Estrada A, Katselis GS, Laarveld B and Barl B: Isolation and evaluation of immunological adjuvant activities of saponins from Polygala senega L. Comp Immunol Microbiol Infect Dis. 23:27–43. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Hartmann G, Marschner A, Viveros PR, et al: CpG oligonucleotides induce strong humoral but only weak CD4+ T cell responses to protein antigens in rhesus macaques in vivo. Vaccine. 23:3310–3317. 2005.PubMed/NCBI

32 

Sobol PT, Boudreau JE, Stephenson K, Wan Y, Lichty BD and Mossman KL: Adaptive antiviral immunity is a determinant of the therapeutic success of oncolytic virotherapy. Mol Ther. 19:335–344. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Kawase O, Goo YK, Jujo H, Nishikawa Y and Xuan X: Starfish, Asterias amurensis and Asterina pectinifera, as potential sources of Th1 immunity-stimulating adjuvants. J Vet Med Sci. 73:227–229. 2011.

34 

Pulendran B and Ahmed R: Translating innate immunity into immunological memory: implications for vaccine development. Cell. 124:849–863. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Sun Y, Liu J, Yu H and Gong C: Isolation and evaluation of immunological adjuvant activities of saponins from the roots of Pulsatilla chinensis with less adverse reactions. Int Immunopharmacol. 10:584–590. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Han Y, Guo Q, Zhang M, Chen Z and Cao X: CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1. J Immunol. 182:111–120. 2009.

37 

Watts C and Powis S: Pathways of antigen processing and presentation. Rev Immunogenet. 1:60–74. 1999.PubMed/NCBI

38 

Insel RA and Anderson PW: Oligosaccharide-protein conjugate vaccines induce and prime for oligoclonal IgG antibody responses to the Haemophilus influenzae b capsular polysaccharide in human infants. J Exp Med. 163:262–269. 1986. View Article : Google Scholar : PubMed/NCBI

39 

Shelly MA, Jacoby H, Riley GJ, Graves BT, Pichichero M and Treanor JJ: Comparison of pneumococcal polysaccharide and CRM197 conjugated pneumococcal oligosaccharide vaccines in young and elderly adults. Infect Immun. 65:242–247. 1997.PubMed/NCBI

40 

Kasper DL, Paoletti LC, Wessels MR, Guttormsen HK, Carey VJ, Jennings HJ and Baker CJ: Immune response to type III group B streptococcal polysaccharide-tetanus toxoid conjugate vaccine. J Clin Invest. 98:2308–2314. 1996. View Article : Google Scholar : PubMed/NCBI

41 

Shiomi H, Masuda A, Nishiumi S, et al: Gamma interferon produced by antigen-specific CD4+ T cells regulates the mucosal immune responses to Citrobacter rodentium infection. Infect Immun. 78:2653–2666. 2010. View Article : Google Scholar : PubMed/NCBI

42 

Meng C, Peng X, Shi X, Wang H and Guo Y: Effects of a chemically derived homo zwitterionic polysaccharide on immune activation in mice. Acta Biochim Biophys Sin (Shanghai). 41:737–744. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cui L, Sun Y, Xu H, Xu H, Cong H and Liu J: A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncol Lett 6: 1039-1044, 2013.
APA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., & Liu, J. (2013). A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncology Letters, 6, 1039-1044. https://doi.org/10.3892/ol.2013.1484
MLA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6.4 (2013): 1039-1044.
Chicago
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6, no. 4 (2013): 1039-1044. https://doi.org/10.3892/ol.2013.1484
Copy and paste a formatted citation
x
Spandidos Publications style
Cui L, Sun Y, Xu H, Xu H, Cong H and Liu J: A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncol Lett 6: 1039-1044, 2013.
APA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., & Liu, J. (2013). A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant. Oncology Letters, 6, 1039-1044. https://doi.org/10.3892/ol.2013.1484
MLA
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6.4 (2013): 1039-1044.
Chicago
Cui, L., Sun, Y., Xu, H., Xu, H., Cong, H., Liu, J."A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant". Oncology Letters 6, no. 4 (2013): 1039-1044. https://doi.org/10.3892/ol.2013.1484
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