Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line

  • Authors:
    • Fei Liu
    • Wei Jiao
    • Xiang‑Lan Mo
    • Jiao Lan
    • Rui‑Ping Xiao
    • Xiang‑Zhen Zhou
    • Zhen‑Lu Huang
    • Xiao‑Min Mo
    • Gang Li
  • View Affiliations

  • Published online on: August 5, 2013     https://doi.org/10.3892/ol.2013.1513
  • Pages: 980-984
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Abstract

The present study aimed to identify the molecular pathological changes of the nasopharyngeal carcinoma (NPC) epithelial CNE3 cell line, which has been used in experimental studies for 20 years in a culture environment. The pathological type of NPC and the presence of the Epstein‑Barr virus (EBV) were identified. CNE3 short tandem repeats (STRs) were amplified, analyzed and compared using metastatic carcinoma tissue from primary NPC. Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to identify the immunophenotype and EBV‑encoded small RNA (EBER) expression in nude mice transplanted CNE3 tumor cells. Polymerase chain reaction (PCR) and DNA sequencing were used to identify the EBV oncogene, BamH1‑A right frame 1 (BARF1) and electron microscopy was used to analyze the organization of the ultrastructure. CNE3 was not cross‑contaminated by other human cell lines and the EBV was no longer present in the CNE3 cells. The pathological type of CNE3 was transformed from an undifferentiated non‑keratinizing carcinoma with focal adenocarcinoma differentiation into a poorly‑differentiated adenocarcinoma. In conclusion, this knowledge on the molecular pathological changes of CNE3 may aid in the development of new research approaches for NPC.
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October 2013
Volume 6 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu F, Jiao W, Mo XL, Lan J, Xiao RP, Zhou XZ, Huang ZL, Mo XM and Li G: Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line. Oncol Lett 6: 980-984, 2013
APA
Liu, F., Jiao, W., Mo, X., Lan, J., Xiao, R., Zhou, X. ... Li, G. (2013). Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line. Oncology Letters, 6, 980-984. https://doi.org/10.3892/ol.2013.1513
MLA
Liu, F., Jiao, W., Mo, X., Lan, J., Xiao, R., Zhou, X., Huang, Z., Mo, X., Li, G."Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line". Oncology Letters 6.4 (2013): 980-984.
Chicago
Liu, F., Jiao, W., Mo, X., Lan, J., Xiao, R., Zhou, X., Huang, Z., Mo, X., Li, G."Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line". Oncology Letters 6, no. 4 (2013): 980-984. https://doi.org/10.3892/ol.2013.1513