Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line

Liu,Fei; Jiao,Wei; Mo,Xiang‑Lan; Lan,Jiao; Xiao,Rui‑Ping; Zhou,Xiang‑Zhen; Huang,Zhen‑Lu; Mo,Xiao‑Min; Li,Gang

doi:10.3892/ol.2013.1513

October 2013 Volume 6 Issue 4

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October 2013 Volume 6 Issue 4

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Article

Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line

Authors:
- Fei Liu
- Wei Jiao
- Xiang‑Lan Mo
- Jiao Lan
- Rui‑Ping Xiao
- Xiang‑Zhen Zhou
- Zhen‑Lu Huang
- Xiao‑Min Mo
- Gang Li
View Affiliations / Copyright

Affiliations: Research Center of Medical Sciences, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Medical University, Nanning 530021, P.R. China, Department of Pathology, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Medical University, Nanning 530021, P.R. China , Laboratory of Electron Microscopy, Guangxi Medical University, Nanning 530021, P.R. China
Pages: 980-984
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Published online on: August 5, 2013

https://doi.org/10.3892/ol.2013.1513
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Abstract

The present study aimed to identify the molecular pathological changes of the nasopharyngeal carcinoma (NPC) epithelial CNE3 cell line, which has been used in experimental studies for 20 years in a culture environment. The pathological type of NPC and the presence of the Epstein‑Barr virus (EBV) were identified. CNE3 short tandem repeats (STRs) were amplified, analyzed and compared using metastatic carcinoma tissue from primary NPC. Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to identify the immunophenotype and EBV‑encoded small RNA (EBER) expression in nude mice transplanted CNE3 tumor cells. Polymerase chain reaction (PCR) and DNA sequencing were used to identify the EBV oncogene, BamH1‑A right frame 1 (BARF1) and electron microscopy was used to analyze the organization of the ultrastructure. CNE3 was not cross‑contaminated by other human cell lines and the EBV was no longer present in the CNE3 cells. The pathological type of CNE3 was transformed from an undifferentiated non‑keratinizing carcinoma with focal adenocarcinoma differentiation into a poorly‑differentiated adenocarcinoma. In conclusion, this knowledge on the molecular pathological changes of CNE3 may aid in the development of new research approaches for NPC.

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1	Huang TR, Zhang SW, Chen WQ, Deng W, Zhang CY, Zhou XJ and Zhai RH: Trends in nasopharyngeal carcinoma mortality in China, 1973–2005. Asian Pac J Cancer Prev. 13:2495–2502. 2012.PubMed/NCBI
2	Jiao W: Establishment of a human epithelial cell line of nasopharyngeal carcinoma - CNE3 and its biological characteristics. Journal of Guangxi Medical University. 12:187–190. 1995.(In Chinese).
3	Chen W, Lee Y, Wang H, Yu GG, Jiao W, Zhou W and Zeng Y: Suppression of human nasopharyngeal carcinoma cell growth in nude mice by the wild-type p53 gene. J Cancer Res Clin Oncol. 119:46–48. 1992. View Article : Google Scholar : PubMed/NCBI
4	Teng ZP, Ooka T, Huang DP and Zeng Y: Detection of Epstein-Barr virus DNA in well and poorly differentiated nasopharyngeal carcinoma cell lines. Virus Genes. 13:53–60. 1996. View Article : Google Scholar : PubMed/NCBI
5	Xia Y, Wong NS, Fona WF and Tideman H: Upregulation of GADD153 expression in the apoptotic signaling of N-(4-hydroxyphenyl)retinamide (4HPR). Int J Cancer. 102:7–14. 2002. View Article : Google Scholar : PubMed/NCBI
6	Peng LX, Chen JX, Cheng JJ, Liu F, et al: Establishment of radioresistant subline from human nasopharyngeal carcinoma cell line by repeating irradiation. Chin J New Clin Med. 5:1107–1109. 2012.(In Chinese).
7	Yang XL, Liu XC, Huang L, Lan J, Zhang HY, Qin MB, Zhong YY and Mo ZN: Effect of TSA on nasopharyngeal carcinoma CNE3 cells and its mechanism. Chin J Public Health. 26:1029–1030. 2010.(In Chinese).
8	Liu XC, Lan J, Nong CZ, Pan LL and Jiao W: Effects of mangiferin on induction of apoptosis and intracellular Ca2+ concentration in nasopharyngeal carcinoma CNE3 cells. Chin J New Clin Med. 3:805–807. 2010.(In Chinese).
9	Tsai ST, Jin YT and Su IJ: Expression of EBER1 in primary and metastatic nasopharyngeal carcinoma tissues using in situ hybridization. A correlation with WHO histologic subtypes. Cancer. 77:231–236. 1996. View Article : Google Scholar : PubMed/NCBI
10	Bay B, Chan Y, Fong C and Leong H: Differential cellular zinc levels in metastatic and primary nasopharyngeal carcinoma. Int J Oncol. 11:745–748. 1997.PubMed/NCBI
11	Lin JJ, He SY, Hao XP and Zong YS: Study on the primary and metastatic tumours of nasopharyngeal carcinoma using microspectrometry and immunohistochemistry. Chin J Cancer. 17:90–92. 1998.(In Chinese).
12	Kaufmann O, Fietze E, Mengs J and Dietel M: Value of p63 and cytokeratin 5/6 as immunohistochemical markers for the differential diagnosis of poorly differentiated and undifferentiated carcinomas. Am J Clin Pathol. 116:823–830. 2001. View Article : Google Scholar : PubMed/NCBI
13	Mitroi M, Cčpitčnescu A, Georgescu CV, Mogoantă CA, Popescu C, Georgescu M, Mitroi G and Ioniţă E: Expression pattern of CK7 and CK20 in nasal polyps, at patients with chronic rhinosinusitis with nasal polyposis. Rom J Morphol Embryol. 52(3 Suppl): S1051–S1057. 2011.PubMed/NCBI
14	Kuo TT, Shih LY and Tsang NM: Nasal NK/T cell lymphoma in Taiwan: a clinicopathologic study of 22 cases, with analysis of histologic subtypes, Epstein-Barr virus LMP-1 gene association, and treatment modalities. Int J Surg Pathol. 12:375–387. 2004. View Article : Google Scholar : PubMed/NCBI
15	Cheung ST, Huang DP, Hui AB, Lo KW, Tsang YS, Wong N, Whitney BM and Lee JC: Nasopharyngeal carcinoma cell line (C666-1) consistently harbouring Esptein-Barr virus. Int J Cancer. 83:121–126. 1999. View Article : Google Scholar : PubMed/NCBI
16	de Turenne-Tessier M and Ooka T: Post-translational modifications of Epstein-Barr virus BARF1 oncogene-encoded polypeptide. J Gen Virol. 88:2656–2661. 2007.PubMed/NCBI
17	Dittmer DP, Hilscher CJ, Gulley ML, Yang EV, Chen M and Glaser R: Multiple pathways for Epstein-Barr virus episome loss from nasopharyngeal carcinoma. Int J Cancer. 123:2105–2112. 2008. View Article : Google Scholar : PubMed/NCBI
18	Sivachandran N, Thawe NN and Frappier L: Epstein-Barr virus nuclear antigen 1 replication and segregation functions in nasopharyngeal carcinoma cell lines. J Virol. 85:10425–10430. 2011. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line

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