Expression of peptidyl‑prolyl isomerase PIN1 and its role in the pathogenesis of extrahepatic cholangiocarcinoma

  • Authors:
    • Urangoo Jamiyandorj
    • Jun Sang Bae
    • Sang Jae Noh
    • Sarangerel Jachin
    • Ji Eun Choi
    • Kyu Yun Jang
    • Myoung Ja Chung
    • Myoung Jae Kang
    • Dong Geun Lee
    • Woo Sung Moon
  • View Affiliations

  • Published online on: August 12, 2013     https://doi.org/10.3892/ol.2013.1525
  • Pages: 1421-1426
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Abstract

The phosphorylation of proteins on serine/threonine residues that immediately precede proline (pSer/Thr‑Pro) is a key signaling mechanism by which cell cycle regulation and cell differentiation and proliferation occur. The peptidyl‑prolyl isomerase PIN1‑catalyzed conformational changes of the pSer/Thr‑Pro motifs may have profound effects on the function of numerous oncogenic and cell signaling pathways. To date, no studies have examined the expression of PIN1 and its potential role in the pathogenesis of extrahepatic cholangiocarcinoma (ECC). Therefore, the present study performed an immunohistochemistry analysis of the expression of PIN1 in 67 cases of ECC and evaluated its association with clinicopathological factors. In addition, the role of PIN1 was examined using synthetic small interfering RNA (siRNA) to silence PIN1 gene expression in human CC RBE cells. Positive PIN1 expression was observed in 35 of the 67 (52.2%) ECC cases and was predominantly localized to the nucleus of the tumor cells. The immunoreactive score for PIN1 was significantly higher in the tumor cells (4.07±0.4) compared with the adjacent benign bile duct cells (1.19±0.4) (P<0.001). PIN1 expression was significantly correlated with tumor cell proliferation (Ki‑67 labeling index; P=0.024). Silencing PIN1 expression using siRNA significantly decreased the proliferation, migration and invasion of the tumor cells. In conclusion, the results indicated that the expression of PIN1 may play a key role in the development and progression of ECC.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jamiyandorj U, Bae JS, Noh SJ, Jachin S, Choi JE, Jang KY, Chung MJ, Kang MJ, Lee DG, Moon WS, Moon WS, et al: Expression of peptidyl‑prolyl isomerase PIN1 and its role in the pathogenesis of extrahepatic cholangiocarcinoma. Oncol Lett 6: 1421-1426, 2013
APA
Jamiyandorj, U., Bae, J.S., Noh, S.J., Jachin, S., Choi, J.E., Jang, K.Y. ... Moon, W.S. (2013). Expression of peptidyl‑prolyl isomerase PIN1 and its role in the pathogenesis of extrahepatic cholangiocarcinoma. Oncology Letters, 6, 1421-1426. https://doi.org/10.3892/ol.2013.1525
MLA
Jamiyandorj, U., Bae, J. S., Noh, S. J., Jachin, S., Choi, J. E., Jang, K. Y., Chung, M. J., Kang, M. J., Lee, D. G., Moon, W. S."Expression of peptidyl‑prolyl isomerase PIN1 and its role in the pathogenesis of extrahepatic cholangiocarcinoma". Oncology Letters 6.5 (2013): 1421-1426.
Chicago
Jamiyandorj, U., Bae, J. S., Noh, S. J., Jachin, S., Choi, J. E., Jang, K. Y., Chung, M. J., Kang, M. J., Lee, D. G., Moon, W. S."Expression of peptidyl‑prolyl isomerase PIN1 and its role in the pathogenesis of extrahepatic cholangiocarcinoma". Oncology Letters 6, no. 5 (2013): 1421-1426. https://doi.org/10.3892/ol.2013.1525