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Article

Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells

  • Authors:
    • Zong‑Gang Xie
    • Ye Xie
    • Qi‑Rong Dong
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 1465-1469
    |
    Published online on: August 16, 2013
       https://doi.org/10.3892/ol.2013.1531
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Abstract

It has been well documented that the inhibition of the mammalian target of rapamycin (mTOR) induces autophagy in proliferative cells. Therefore, mTOR inhibitors have been proposed for the treatment of cancer. As autophagy plays significant roles in tumor cell survival, the present study aimed to investigate the contribution of autophagy activation to the antitumor effects of cis‑diamminedichloroplatinum (CDDP). An MTT assay was used to determine the cytotoxic effects of rapamycin on MG63 osteosarcoma cells. The cell cycle was assessed using a flow cytometry analysis subsequent to staining the DNA with propidium iodide. The mitochondrial membrane potential (Δψ) was measured using the fluorescent probe, JC‑1. Western blot analysis was used to determine the expression of the proteins that are involved in apoptosis and autophagy, including p53, p62, light chain 3 (LC3) and Beclin‑1. The viability of the MG63 cells was inhibited following rapamycin or CDDP treatment. The mitochondrial Δψ collapsed following treatment with rapamycin or CDDP. Rapamycin induced cell death and enhanced the effects of the induction of MG63 cell death by CDDP. Western blot analysis detected the induced expression of the p53 and Beclin‑1 proteins and the autophagic proteins, LC3 and p62. Rapamycin was observed to induce the death of cancer cells through apoptotic and autophagic mechanisms. Rapamycin may enhance the effects of the activation of autophagy and the induction of apoptosis by CDDP.
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Copy and paste a formatted citation
Spandidos Publications style
Xie ZG, Xie Y and Dong QR: Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells. Oncol Lett 6: 1465-1469, 2013.
APA
Xie, Z., Xie, Y., & Dong, Q. (2013). Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells. Oncology Letters, 6, 1465-1469. https://doi.org/10.3892/ol.2013.1531
MLA
Xie, Z., Xie, Y., Dong, Q."Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells". Oncology Letters 6.5 (2013): 1465-1469.
Chicago
Xie, Z., Xie, Y., Dong, Q."Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells". Oncology Letters 6, no. 5 (2013): 1465-1469. https://doi.org/10.3892/ol.2013.1531
Copy and paste a formatted citation
x
Spandidos Publications style
Xie ZG, Xie Y and Dong QR: Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells. Oncol Lett 6: 1465-1469, 2013.
APA
Xie, Z., Xie, Y., & Dong, Q. (2013). Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells. Oncology Letters, 6, 1465-1469. https://doi.org/10.3892/ol.2013.1531
MLA
Xie, Z., Xie, Y., Dong, Q."Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells". Oncology Letters 6.5 (2013): 1465-1469.
Chicago
Xie, Z., Xie, Y., Dong, Q."Inhibition of the mammalian target of rapamycin leads to autophagy activation and cell death of MG63 osteosarcoma cells". Oncology Letters 6, no. 5 (2013): 1465-1469. https://doi.org/10.3892/ol.2013.1531
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