KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients

  • Authors:
    • Cristina Gutiérrez
    • Javier Rodriguez
    • Ana Patiño-García
    • Jesús García-Foncillas
    • Josefa Salgado
  • View Affiliations

  • Published online on: August 23, 2013     https://doi.org/10.3892/ol.2013.1544
  • Pages: 1343-1345
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study describes an optimized method for isolating peripheral blood circulating tumor cells (CTCs) and performing KRAS mutation analysis. The approach combines isolation of peripheral blood mononuclear cells and immunomagnetic labeling with CD45 and CD326 human microbeads with KRAS analysis performed with a Therascreen KRAS kit by quantitative PCR. KRAS mutations were detected in the CTCs of patients with metastatic colorectal cancer (mCRC). CTCs may represent an alternative to invasive procedures and their analysis may be representative of the current disease status of the patient. This proposed analysis may be performed in a daily clinical practice.
View Figures
View References

Related Articles

Journal Cover

November 2013
Volume 6 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., & Salgado, J. (2013). KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients. Oncology Letters, 6, 1343-1345. https://doi.org/10.3892/ol.2013.1544
MLA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6.5 (2013): 1343-1345.
Chicago
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6, no. 5 (2013): 1343-1345. https://doi.org/10.3892/ol.2013.1544