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November 2013 Volume 6 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients

  • Authors:
    • Cristina Gutiérrez
    • Javier Rodriguez
    • Ana Patiño-García
    • Jesús García-Foncillas
    • Josefa Salgado
  • View Affiliations / Copyright

    Affiliations: Clinical Genetics Unit, University Clinic of Navarra, Pamplona, Navarra 31008, Spain, Department of Oncology, University Clinic of Navarra, Pamplona, Navarra 31008, Spain, Jiménez Díaz Foundation, Madrid 28040, Spain
  • Pages: 1343-1345
    |
    Published online on: August 23, 2013
       https://doi.org/10.3892/ol.2013.1544
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Abstract

The present study describes an optimized method for isolating peripheral blood circulating tumor cells (CTCs) and performing KRAS mutation analysis. The approach combines isolation of peripheral blood mononuclear cells and immunomagnetic labeling with CD45 and CD326 human microbeads with KRAS analysis performed with a Therascreen KRAS kit by quantitative PCR. KRAS mutations were detected in the CTCs of patients with metastatic colorectal cancer (mCRC). CTCs may represent an alternative to invasive procedures and their analysis may be representative of the current disease status of the patient. This proposed analysis may be performed in a daily clinical practice.
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Copy and paste a formatted citation
Spandidos Publications style
Gutiérrez C, Rodriguez J, Patiño-García A, García-Foncillas J and Salgado J: KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients. Oncol Lett 6: 1343-1345, 2013.
APA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., & Salgado, J. (2013). KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients. Oncology Letters, 6, 1343-1345. https://doi.org/10.3892/ol.2013.1544
MLA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6.5 (2013): 1343-1345.
Chicago
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6, no. 5 (2013): 1343-1345. https://doi.org/10.3892/ol.2013.1544
Copy and paste a formatted citation
x
Spandidos Publications style
Gutiérrez C, Rodriguez J, Patiño-García A, García-Foncillas J and Salgado J: KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients. Oncol Lett 6: 1343-1345, 2013.
APA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., & Salgado, J. (2013). KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients. Oncology Letters, 6, 1343-1345. https://doi.org/10.3892/ol.2013.1544
MLA
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6.5 (2013): 1343-1345.
Chicago
Gutiérrez, C., Rodriguez, J., Patiño-García, A., García-Foncillas, J., Salgado, J."KRAS mutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients". Oncology Letters 6, no. 5 (2013): 1343-1345. https://doi.org/10.3892/ol.2013.1544
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