Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene

  • Authors:
    • Li Yu
    • Yu Fan
    • Baoding Chen
    • Yue Hu
    • Yina Gao
    • Da Wei
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  • Published online on: September 12, 2013     https://doi.org/10.3892/ol.2013.1572
  • Pages: 1338-1342
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Abstract

The present study aimed to ascertain whether suppression of midkine (MK) expression in pancreatic cancer cells inhibits metastasis to the liver. Human pancreatic cancer AsPC‑1 cells were transfected with small interfering RNA (siRNA) targeting MK. siRNA against MK was observed to reduce the expression of MK mRNA and protein in a concentration‑ and time‑dependent manner, and to decrease the number of migrating and tissue‑penetrating cells in a concentration‑dependent manner (P<0.005). Extracellular vascular endothelial growth factor (VEGF) concentrations were markedly reduced for the siRNA‑transfected cells compared with those that were non‑siRNA‑transfected. The liver transmission rate and tumor nodule number in the animals harboring the siRNA‑transfected cells were lower compared with those in the animals harboring the non‑siRNA‑transfected cells (P<0.005). These data indicate that metastasis of pancreatic cancer cells to the liver requires the expression of MK. The downregulation of VEGF expression is essential to the mechanism whereby suppression of MK expression constrains the metastasis of pancreatic cancer cells to the liver.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu L, Fan Y, Chen B, Hu Y, Gao Y and Wei D: Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene. Oncol Lett 6: 1338-1342, 2013
APA
Yu, L., Fan, Y., Chen, B., Hu, Y., Gao, Y., & Wei, D. (2013). Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene. Oncology Letters, 6, 1338-1342. https://doi.org/10.3892/ol.2013.1572
MLA
Yu, L., Fan, Y., Chen, B., Hu, Y., Gao, Y., Wei, D."Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene". Oncology Letters 6.5 (2013): 1338-1342.
Chicago
Yu, L., Fan, Y., Chen, B., Hu, Y., Gao, Y., Wei, D."Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene". Oncology Letters 6, no. 5 (2013): 1338-1342. https://doi.org/10.3892/ol.2013.1572