Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene

Yu,Li; Fan,Yu; Chen,Baoding; Hu,Yue; Gao,Yina; Wei,Da

doi:10.3892/ol.2013.1572

November 2013 Volume 6 Issue 5

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November 2013 Volume 6 Issue 5

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Article

Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene

Authors:
- Li Yu
- Yu Fan
- Baoding Chen
- Yue Hu
- Yina Gao
- Da Wei
View Affiliations / Copyright

Affiliations: Zhenjiang Key Laboratory of Molecular Endocrinology, Nanjing, Jiangsu 210009, P.R. China, Cancer Institute, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Department of General Surgery, Jiangsu Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China
Pages: 1338-1342
|
Published online on: September 12, 2013

https://doi.org/10.3892/ol.2013.1572
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Abstract

The present study aimed to ascertain whether suppression of midkine (MK) expression in pancreatic cancer cells inhibits metastasis to the liver. Human pancreatic cancer AsPC‑1 cells were transfected with small interfering RNA (siRNA) targeting MK. siRNA against MK was observed to reduce the expression of MK mRNA and protein in a concentration‑ and time‑dependent manner, and to decrease the number of migrating and tissue‑penetrating cells in a concentration‑dependent manner (P<0.005). Extracellular vascular endothelial growth factor (VEGF) concentrations were markedly reduced for the siRNA‑transfected cells compared with those that were non‑siRNA‑transfected. The liver transmission rate and tumor nodule number in the animals harboring the siRNA‑transfected cells were lower compared with those in the animals harboring the non‑siRNA‑transfected cells (P<0.005). These data indicate that metastasis of pancreatic cancer cells to the liver requires the expression of MK. The downregulation of VEGF expression is essential to the mechanism whereby suppression of MK expression constrains the metastasis of pancreatic cancer cells to the liver.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA‑mediated targeting of the midkine gene

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