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December 2013 Volume 6 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

  • Authors:
    • Shangwen Dong
    • Peng Zhang
    • Shaojie Liang
    • Shuo Wang
    • Pei Sun
    • Yuanguo Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Heping, Tianjin 300052, P.R. China, Tianjin Institute of Endocrinology, Tianjin Medical University, Heping, Tianjin 300070, P.R. China
  • Pages: 1656-1662
    |
    Published online on: October 9, 2013
       https://doi.org/10.3892/ol.2013.1608
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Abstract

The tumor suppressor protein retinoblastoma protein‑interacting zinc finger gene 1 (RIZ1) is downregulated in several types of cancer, including esophageal squamous cell carcinoma (ESCC). The present study used two in vitro methods to re‑express RIZ1 in the human ESCC TE13 cell line in order to induce apoptosis. RIZ1 was re‑expressed in the TE13 cells by reintroducing the gene through transfection or by removal of transcriptional repression through treatment with a DNA methyltransferase (DNMT) inhibitor. To reintroduce the gene, the open reading frame of the RIZ1 gene was inserted into the eukaryotic expression pcDNA3.1(+) vector and pcDNA3.1(+)/RIZ1 was purified and transfected into the TE13 ESCC cells. Removing transcriptional repression involved treating the TE13 cells with 5‑aza‑2'‑deoxycytidine (5‑aza‑CdR), a DNMT inhibitor. RIZ1 mRNA and protein expression were determined by quantitative polymerase chain reaction (qPCR) and western blotting. The rate of apoptosis of the cells was determined by flow cytometry. A recombinant eukaryotic human RIZ1 expression plasmid, pcDNA3.1(+)/RIZ1, was constructed and confirmed by sequencing. RIZ1 mRNA and protein expression increased in pcDNA3.1(+)/RIZ1 stably transfected cells. Treatment with 5‑aza‑CdR for 48 and 72 h resulted in increased RIZ1 protein expression and increased the rate of apoptosis in the TE13 cells (P<0.01). In conclusion, transfection of the TE13 cells with the eukaryotic pcDNA3.1(+)/RIZ1 expression vector and reversal of transcriptional repression of RIZ1 using 5‑aza‑CdR demonstrate that it is possible to re‑express RIZ1 in TE13 cells. Furthermore, the re‑expression of RIZ1 led to an increased rate of apoptosis and this method may provide new therapeutic possibilities.
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1 

Amin HM, Hoshino K, Yang H, Lin Q, Lai R and Garcia-Manero G: Decreased expression level of SH2 domain-containing protein tyrosine phosphatase-1 (Shp1) is associated with progression of chronic myeloid leukaemia. J Pathol. 212:402–410. 2007. View Article : Google Scholar

2 

Takeno S, Yamashita SI, Yamamoto S, Takahashi Y, Moroga T, et al: Number of metastasis-positive lymph node stations is a simple and reliable prognostic factor following surgery in patients with esophageal cancer. Exp Ther Med. 4:1087–1091. 2012.PubMed/NCBI

3 

Dong SW, Cui YT, Zhong RR, Liang DC, Liu YM, Wang YG, He Z, Wang S, Liang SJ and Zhang P: Decreased expression of retinoblastoma protein-interacting zinc-finger gene 1 in human esophageal squamous cell cancer by DNA methylation. Clin Lab. 58:41–51. 2012.PubMed/NCBI

4 

Dong SW, Zhang P, Liu YM, et al: Study on RIZ1 gene promoter methylation status in human esophageal squamous cell carcinoma. World J Gastroenterol. 18:576–582. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Klintschar M and Neuhuber F: Evaluation of an alkaline lysis method for the extraction of DNA from whole blood and forensic stains for STR analysis. J Forensic Sci. 45:669–673. 2000.PubMed/NCBI

6 

Dean DA and Gasiorowski JZ: Liposome-mediated transfection. Cold Spring Harb Protoc. 2011:prot55832011.PubMed/NCBI

7 

Buyse IM, Shao G and Huang S: The retinoblastoma protein binds to RIZ, a zincfinger protein that shares an epitope with the adenovirus EIA protein. Proc Natl Acad Sci USA. 92:4467–4471. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Ng WT, Choi CW, Lee MC, Chan SH, Yau TK and Lee AW: Familial nasopharyngeal carcinoma in Hong Kong: epidemiology and implication in screening. Fam Cancer. 8:103–108. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Szende B and Tyihák E: Effect of formaldehyde on cell proliferation and death. Cell Biol Int. 34:1273–1282. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Overmeer RM, Louwers JA, Meijer CJ, van Kemenade FJ, Hesselink AT, et al: Combined CADM1 and MAL promoter methylation analysis to detect (pre-)malignant cervical lesions in high-risk HPV-positive women. Int J Cancer. 129:2218–2225. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Overmeer RM, Henken FE, Snijders PJ, Claassen-Kramer D, Berkhof J, et al: Association between dense CADM1 promoter methylation and reduced protein expression in high-grade CIN and cervical SCC. J Pathol. 215:388–397. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Brait M, Ford JG, Papaiahgari S, Garza MA, Lee JI, et al: Association between lifestyle factors and CpG island methylation in a cancer-free population. Cancer Epidemiol Biomarkers Prev. 18:2984–2991. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Schoenhals M, Kassambara A, De Vos J, Hose D, Moreaux J and Klein B: Embryonic stem cell markers expression in cancers. Biochem Biophys Res Commun. 383:157–162. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Zhang S, Balch C, Chan MW, Lai HC, Matei D, et al: Identification and characterization of ovarian cancer-initiating cells from primary human tumors. Cancer Res. 68:4311–4320. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Chen L, Ling BB, Alcorn J and Yang J: Analysis of the expression of human N-myristoyltransferase 1 (hNMT-1) in cancers. Open Biomarkers J. 2:6–10. 2009. View Article : Google Scholar

16 

Dalerba P, Cho RW and Clarke MF: Cancer stem cells: models and concepts. Annu Rev Med. 58:267–284. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Noman AS, Koide N, Iftakhar-E-Khuda I, Dagvadorj J, Tumurkhuu G, et al: Retinoblastoma protein-interacting zinc finger 1 (RIZ1) participates in RANKL-induced osteoclast formation via regulation of NFATc1 expression. Immunol Lett. 131:166–169. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Philippidis A: Gene therapy briefs. Hum Gene Ther. 23:1221–1223. 2012. View Article : Google Scholar

19 

Dong SW, Ma L, Xu N, Yan HQ, Liu HY, Li YW and Zhang P: Research on the reactivation of Syk expression caused by the inhibition of DNA promoter methylation in the lung cancer. Neoplasma. 58:89–95. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Moran NL: First gene therapy approved. Nat Biotechnol. 30:11532012. View Article : Google Scholar

21 

Fujita J, Crane AM, Souza MK, Dejosez M, Kyba M, et al: Caspase activity mediates the differentiation ofembryonic stem cells. Cell Stem Cell. 2:595–601. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, et al: Induced pluripotent stem cell lines derived from human somatic cells. Science. 318:1917–1920. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dong S, Zhang P, Liang S, Wang S, Sun P and Wang Y: The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells. Oncol Lett 6: 1656-1662, 2013.
APA
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., & Wang, Y. (2013). The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells. Oncology Letters, 6, 1656-1662. https://doi.org/10.3892/ol.2013.1608
MLA
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., Wang, Y."The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells". Oncology Letters 6.6 (2013): 1656-1662.
Chicago
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., Wang, Y."The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells". Oncology Letters 6, no. 6 (2013): 1656-1662. https://doi.org/10.3892/ol.2013.1608
Copy and paste a formatted citation
x
Spandidos Publications style
Dong S, Zhang P, Liang S, Wang S, Sun P and Wang Y: The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells. Oncol Lett 6: 1656-1662, 2013.
APA
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., & Wang, Y. (2013). The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells. Oncology Letters, 6, 1656-1662. https://doi.org/10.3892/ol.2013.1608
MLA
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., Wang, Y."The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells". Oncology Letters 6.6 (2013): 1656-1662.
Chicago
Dong, S., Zhang, P., Liang, S., Wang, S., Sun, P., Wang, Y."The role of the retinoblastoma protein‑interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells". Oncology Letters 6, no. 6 (2013): 1656-1662. https://doi.org/10.3892/ol.2013.1608
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