microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2

Wei,Bin; Song,Ying; Zhang,Yonghong; Hu,Mingjun

doi:10.3892/ol.2013.1609

microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2

Authors:
- Bin Wei
- Ying Song
- Yonghong Zhang
- Mingjun Hu
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Affiliations: Department of Gastroenterology, Xi'an First Hospital, Xi'an, Shaanxi 710002, P.R. China, Department of Gastroenterology, Central Hospital of Xi'an City, Xi'an, Shaanxi 710003, P.R. China
Published online on: October 9, 2013 https://doi.org/10.3892/ol.2013.1609
Pages: 1713-1718

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Abstract

microRNAs (miRNAs or miRs) may function as oncogenes or tumor suppressors. The present study identified that miR‑449a was downregulated in human gastric cancer. The overexpression of miR‑449a inhibited gastric adenocarcinoma cell growth and promoted cell apoptosis in the MGC-803 and SGC-7901 gastric adenocarcinoma cell lines. Subsequently, Bcl‑2 was identified as a potential miR‑449a target by bioinformatics analysis. It was also shown that Bcl‑2 was negatively regulated by miR‑449a at the post‑transcriptional level, via a specific target site within the 3'-untranslated region (3'UTR), by luciferase reporter assay. The expression of miR‑449a induced cell apoptosis, as observed by TdT-mediated dUTP nick end labeling and caspase 3/7 assays, and was rescued by Bcl‑2 expression. Therefore, these observations indicate that miR‑449a acts as a tumor suppressor by targeting the Bcl‑2 gene and that it promotes gastric adenocarcinoma cell apoptosis via Bcl‑2. The findings of this study contribute to or current understanding of the functions of miR‑449a in gastric adenocarcinoma.

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