Mesenchymal stem cells derived from breast cancer tissue promote the proliferation and migration of the MCF‑7 cell line in vitro

Zhang,Chunfu; Zhai,Wei; Xie,Yan; Chen,Qiaolin; Zhu,Wei; Sun,Xiaochun

doi:10.3892/ol.2013.1619

Mesenchymal stem cells derived from breast cancer tissue promote the proliferation and migration of the MCF‑7 cell line in vitro

Authors:
- Chunfu Zhang
- Wei Zhai
- Yan Xie
- Qiaolin Chen
- Wei Zhu
- Xiaochun Sun
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Affiliations: The Second People's Hospital of Kunshan, Kunshan, Jiangsu 215300, P.R. China, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
Published online on: October 11, 2013 https://doi.org/10.3892/ol.2013.1619
Pages: 1577-1582

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Abstract

Mesenchymal stem cells (MSCs) are critical in promoting cancer progression, including tumor growth and metastasis. MSCs, as a subpopulation of cells found in the tumor microenvironment, have been isolated from several tumor tissues, but have not been isolated from breast cancer tissue to date. Therefore, the purpose of this study was to isolate MSCs from primary human breast cancer tissue, and to study the effect of breast cancer MSCs (BC‑MSCs) on the proliferation and migration of the MCF‑7 cell line in vitro. MSCs were isolated and identified from primary breast cancer tissue obtained from 9 patients. The MCF‑7 cell line was treated with 10 and 20% breast cancer‑associated MSC (BC‑MSC)‑conditioned medium (CM) for 10‑48 h, and changes in proliferation and migration were observed. Furthermore, we investigated the migration of 10 and 20% CM concentrations on MCF‑7 through a scratch wound assay and a transwell migration assay. We successfully isolated and identified MSCs from primary breast cancer tissues. BC‑MSCs showed characteristics similar to those of bone marrow MSCs, and possessed the capability of multipotential differentiation into osteoblasts and adipocytes. The results of the 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay showed that 10 and 20% CM concentrations increased the proliferation of MCF‑7 cells to different levels. The results also revealed a greater increase in different levels compared with the control group. In conclusion, MSCs were confirmed to exist in human breast cancer tissues, and BC‑MSCs may promote the proliferation and migration of breast cancer cells.

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