Expression of p‑Akt in ovarian serous carcinoma and its association with proliferation and apoptosis

Song,Tieying; Wang,Liwen; Mo,Zhongfu; Mao,Limei; Ma,Xiaojing; Niu,Runling; Gu,Kunfeng; Yan,Ruyu; Ma,Pengyu; Qi,Yan; Jiao,Qingfang

doi:10.3892/ol.2013.1641

2014-January Volume 7 Issue 1

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2014-January Volume 7 Issue 1

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Article

Expression of p‑Akt in ovarian serous carcinoma and its association with proliferation and apoptosis

Authors:
- Tieying Song
- Liwen Wang
- Zhongfu Mo
- Limei Mao
- Xiaojing Ma
- Runling Niu
- Kunfeng Gu
- Ruyu Yan
- Pengyu Ma
- Yan Qi
- Qingfang Jiao
View Affiliations / Copyright

Affiliations: Department of Anesthesiology, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China, Department of Gynecology, The Sixth Hospital of Shijiazhuang, Shijiazhuang, Hebei 050000, P.R. China, Department of Neurosurgery, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China
Pages: 59-64
|
Published online on: October 25, 2013

https://doi.org/10.3892/ol.2013.1641
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Abstract

The aim of the present study was to determine the expression of p‑Akt in ovarian serous carcinoma (OSC) and its association with proliferation and apoptosis. Paraffin‑embedded tissues of patients aged between 35 and 64 years old without history of radiotherapy, chemotherapy and hormone therapy prior to surgery were collected. In total, samples included 12 ovarian serous cystadenomas (OSAs), 18 ovarian serous borderline tumors (OS‑BTs) and 46 OSCs. Of the 46 OSC samples, 16 were well‑differentiated, 20 were moderately differentiated and 10 were poorly differentiated, while 22 developed lymphatic metastases and 24 were metastasis‑free. An additional 10 paraffin‑embedded normal ovarian tissues (NOTs) were used as controls. Streptavidin-peroxidase immunohistochemistry assays were used to investigate the expression of p‑Akt and cyclin D1 in the collected samples. Compared with NOT, p‑Akt expression in the OS‑BT and OSC groups, as well as cyclin D1 expression in the OSA and OSC groups, was significantly elevated (P<0.05). Compared with the OSA group, p‑Akt expression in the OSC group was significantly elevated (P<0.01) and reversely associated with tumor differentiation (P<0.01), whereas cyclin D1 expression showed no correlation with tumor differentiation (P>0.05). The expression of p‑Akt, caspase‑3 and cyclin D1 was positively associated with lymphatic metastasis (r=0.334; P=0.023). The expression of p‑Akt gradually increased with carcinoma development and was associated with differentiation and metastasis of OSC, revealing that the activation of the PI3K/Akt signaling pathway is involved in the development of OSC. Furthermore, the expression of cyclin D1 gradually increased in the NOT, OSA, OS‑BT and OSC groups and was associated with tumor metastasis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of p‑Akt in ovarian serous carcinoma and its association with proliferation and apoptosis

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