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2014-January Volume 7 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection

  • Authors:
    • Chuan Xie
    • Li‑Yao Xu
    • Zhen Yang
    • Xi‑Mei Cao
    • Wei Li
    • Nong‑Hua Lu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 159-163
    |
    Published online on: November 20, 2013
       https://doi.org/10.3892/ol.2013.1693
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Abstract

Phosphorylation of H2AX at Ser 139 (γH2AX) is a biomarker of DNA double‑strand breaks (DSBs). The present study aimed to explore the association between γH2AX levels and gastric pathology and Helicobacter pylori (H. pylori) infection. Gastric biopsies were obtained from 302 H. pylori‑negative and ‑positive patients, including those with chronic gastritis (CG), intestinal metaplasia (IM), dysplasia (Dys) and gastric cancer (GC). Proteins were extracted from five gastric epithelial cell lines and from 10 specimens of matched GC and adjacent normal tissues. The expression of γH2AX, a biomarker for the detection of DNA DSBs, in gastric tissues was detected by immunohistochemistry and western blotting. The expression of γH2AX progressively increased in tissues according to pathological stage from CG to Dys, but was slightly decreased in GC. H. pylori infection was associated with increased γH2AX expression, IM and Dys. Overexpression of γH2AX in GC was found to correlate with tumor location, gross appearance, differentiation, depth of invasion, TNM stage and lymph node metastasis. The results indicated that DSBs appear to be an early molecular event in gastric carcinogenesis, which may be associated with H. pylori infection. Moreover, immunohistochemical staining of γH2AX was found to correlate with a number of clinicopathological characteristics. The expression of γH2AX may serve as a valuable biomarker for the diagnosis and progression of GC.
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Copy and paste a formatted citation
Spandidos Publications style
Xie C, Xu LY, Yang Z, Cao XM, Li W and Lu NH: Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection. Oncol Lett 7: 159-163, 2014.
APA
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., & Lu, N. (2014). Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection. Oncology Letters, 7, 159-163. https://doi.org/10.3892/ol.2013.1693
MLA
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., Lu, N."Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection". Oncology Letters 7.1 (2014): 159-163.
Chicago
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., Lu, N."Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection". Oncology Letters 7, no. 1 (2014): 159-163. https://doi.org/10.3892/ol.2013.1693
Copy and paste a formatted citation
x
Spandidos Publications style
Xie C, Xu LY, Yang Z, Cao XM, Li W and Lu NH: Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection. Oncol Lett 7: 159-163, 2014.
APA
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., & Lu, N. (2014). Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection. Oncology Letters, 7, 159-163. https://doi.org/10.3892/ol.2013.1693
MLA
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., Lu, N."Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection". Oncology Letters 7.1 (2014): 159-163.
Chicago
Xie, C., Xu, L., Yang, Z., Cao, X., Li, W., Lu, N."Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection". Oncology Letters 7, no. 1 (2014): 159-163. https://doi.org/10.3892/ol.2013.1693
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