β‑diketone‑cobalt complexes inhibit DNA synthesis and induce S‑phase arrest in rat C6 glioma cells

Zhang,Kaizhi; Zhao,Xingli; Liu,Junzhi; Fang,Xiangyang; Wang,Xuepeng; Wang,Xiaohong; Li,Rui

doi:10.3892/ol.2013.1772

β‑diketone‑cobalt complexes inhibit DNA synthesis and induce S‑phase arrest in rat C6 glioma cells

Authors:
- Kaizhi Zhang
- Xingli Zhao
- Junzhi Liu
- Xiangyang Fang
- Xuepeng Wang
- Xiaohong Wang
- Rui Li
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Affiliations: China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Key Laboratory of Polyoxometalate Science of Ministry of Education, Northeast Normal University, Changchun, Jilin 130024, P.R. China
Published online on: December 24, 2013 https://doi.org/10.3892/ol.2013.1772
Pages: 881-885

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Abstract

β‑diketone‑cobalt complexes, a family of newly synthesized non‑platinum metal compounds, exhibit potential antitumor activity; however, the antitumor mechanism is unclear. The current study investigated the mechanism by which β‑diketone‑cobalt complexes inhibit rat C6 glioma cell proliferation. It was found that β‑diketone‑cobalt complexes suppress rat C6 glioma cell viability in a dose‑dependent manner (3.125‑100 µg/ml). In rat C6 glioma cells, the IC50 value of β‑diketone‑cobalt complexes was 24.7±3.395 µg/ml and the IC10 value was 4.37±1.53 µg/ml, indicating a strong inhibitory effect. Further investigation suggested that β‑diketone‑cobalt complexes inhibit rat C6 glioma cell proliferation, which is associated with S‑phase arrest and DNA synthesis inhibition. During this process, β‑diketone‑cobalt complexes decreased cyclin A expression and increased cyclin E and p21 expression. In addition, β‑diketone‑cobalt complexes exhibit a stronger antitumor capability than the antineoplastic agent, 5‑fluorouracil.

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