Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells

  • Authors:
    • Xuemei Chen
    • Di Liu
    • Junfu Wang
    • Qinghong Su
    • Peng Zhou
    • Jinsheng Liu
    • Meng Luan
    • Xiaoqun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, The Second Affiliated Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China
  • Pages: 771-777
    |
    Published online on: January 10, 2014
       https://doi.org/10.3892/ol.2014.1789
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The melanoma differentiation‑associated gene‑7 [MDA‑7; renamed interleukin (IL)‑24] was isolated from human melanoma cells induced to terminally differentiate by treatment with interferon and mezerein. MDA‑7/IL‑24 functions as a multimodality anticancer agent, possessing proapoptotic, antiangiogenic and immunostimulatory properties. All these attributes make MDA‑7/IL‑24 an ideal candidate for cancer gene therapy. In the present study, the human MDA‑7/IL‑24 gene was transfected into the human laryngeal cancer Hep‑2 cell line and human umbilical vein endothelial cells (HUVECs) with a replication‑incompetent adenovirus vector. Reverse transcription polymerase chain reaction and western blot analysis confirmed that the Ad‑hIL‑24 was expressed in the two cells. The expression of the antiapoptotic gene, Bcl‑2, was significantly decreased and the IL‑24 receptor was markedly expressed in Hep‑2 cells following infection with Ad‑hIL‑24, but not in HUVECs. In addition, the expression of the proapoptotic gene, Bax, was induced and the expression of caspase‑3 was increased in the Hep‑2 cells and HUVECs. Methyl thiazolyl tetrazolium assay indicated that Ad‑hIL‑24 may induce growth suppression in Hep‑2 cells but not in HUVECs. In conclusion, Ad‑hIL‑24 selectively inhibits proliferation and induces apoptosis in Hep‑2 cells. No visible damage was found in HUVECs. Therefore, the results of the current study indicated that Ad‑hIL‑24 may have a potent suppressive effect on human laryngeal carcinoma cell lines, but is safe for healthy cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Karatzanis AD, Psychogios G, Zenk J, Waldfahrer F, Hornung J, Velegrakis GA and Iro H: Comparison among different available surgical approaches in T1 glottic cancer. Laryngoscope. 119:1704–1708. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Lebedeva IV, Sauane M, Gopalkrishnan RV, et al: MDA-7/IL-24: exploiting cancer’s Achilles’ heel. Mol Ther. 11:4–18. 2005.

3 

Gupta P, Su ZZ, Lebedeva IV, et al: mda-7/IL-24: multifunctional cancer-specific apoptosis-inducing cytokine. Pharmacol Ther. 111:596–628. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Fisher PB: Is mda-7/IL-24 a ‘magic bullet’ for cancer? Cancer Res. 65:10128–10138. 2005.

5 

Jiang H, Lin JJ, Su ZZ, Goldstein NI and Fisher PB: Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene. 11:2477–2486. 1995.

6 

Wei N, Fan JK, Gu JF and Liu XY: Double-regulated oncolytic adenovirus-mediated interleukin-24 overexpression exhibits potent antitumor activity on gastric adenocarcinoma. Hum Gene Ther. 21:855–864. 2010. View Article : Google Scholar

7 

Huang EY, Madireddi MT, Gopalkrishnan RV, et al: Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties. Oncogene. 20:7051–7063. 2001. View Article : Google Scholar

8 

Sauane M, Gupta P, Lebedeva IV, et al: N-glycosylation of MDA-7/IL-24 is dispensable for tumor cell-specific apoptosis and ‘bystander’ antitumor activity. Cancer Res. 66:11869–11877. 2006.PubMed/NCBI

9 

Jiang H, Su ZZ, Lin JJ, Goldstein NI, Young CS and Fisher PB: The melanoma differentiation associated gene mda-7 suppresses cancer cell growth. Proc Natl Acad Sci USA. 93:9160–9165. 1996. View Article : Google Scholar : PubMed/NCBI

10 

Tian Z, Shen X, Feng H and Gao B: IL-1 beta attenuates IFN-alpha beta-induced antiviral activity and STAT1 activation in the liver: involvement of proteasome- dependent pathway. J Immunol. 165:3959–3965. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R and Dalloul A: Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Blood. 115:1718–1726. 2010. View Article : Google Scholar

12 

Ekmekcioglu S, Ellerhorst J, Mhashilkar AM, et al: Down-regulated melanoma differentiation associated gene (mda-7) expression in human melanomas. Int J Cancer. 94:54–59. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Ellerhorst JA, Prieto VG, Ekmekcioglu S, Broemeling L, Yekell S, Chada S and Grimm EA: Loss of MDA-7 expression with progression of melanoma. J Clin Oncol. 20:1069–1074. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Su ZZ, Madireddi MT, Lin JJ, et al: The cancer growth suppressor gene mda-7 selectively induces apoptosis in human breast cancer cells and inhibits tumor growth in nude mice. Proc Natl Acad Sci USA. 95:14400–14405. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Saeki T, Mhashilkar A, Chada S, Branch C, Roth JA and Ramesh R: Tumor-suppressive effects by adenovirus-mediated mda-7 gene transfer in non-small cell lung cancer cell in vitro. Gene Ther. 7:2051–2057. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Madireddi MT, Su ZZ, Young CS, Goldstein NI and Fisher PB: Mda-7, a novel melanoma differentiation associated gene with promise for cancer gene therapy. Adv Exp Med Biol. 465:239–261. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Lebedeva IV, Su ZZ, Chang Y, Kitada S, Reed JC and Fisher PB: The cancer growth suppressing gene mda-7 induces apoptosis selectively in human melanoma cells. Oncogene. 21:708–718. 2002. View Article : Google Scholar

18 

Su ZZ, Lebedeva IV, Sarkar D, et al: Melanoma differentiation associated gene-7, mda-7/IL-24, selectively induces growth suppression, apoptosis and radiosensitization in malignant gliomas in a p53-independent manner. Oncogene. 22:1164–1180. 2003. View Article : Google Scholar

19 

Lebedeva IV, Su ZZ, Vozhilla N, et al: Mechanism of in vitro pancreatic cancer cell growth inhibition by melanoma differentiation-associated gene-7/interleukin-24 and perillyl alcohol. Cancer Res. 68:7439–7447. 2008. View Article : Google Scholar

20 

Fisher PB, Gopalkrishnan RV, Chada S, et al: mda-7/IL-24, a novel cancer selective apoptosis inducing cytokine gene: from the laboratory into the clinic. Cancer Biol Ther. 2(4 Suppl 1): S23–S37. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Cunningham CC, Chada S, Merritt JA, et al: Clinical and local biological effects of an intratumoral injection of mda-7 (IL24; INGN 241) in patients with advanced carcinoma: a phase I study. Mol Ther. 11:149–159. 2005. View Article : Google Scholar

22 

Tong AW, Nemunaitis J, Su D, et al: Intratumoral injection of INGN 241, a nonreplicating adenovector expressing the melanoma-differentiation associated gene-7 (mda-7/IL24): biologic outcome in advanced cancer patients. Mol Ther. 11:160–172. 2005. View Article : Google Scholar

23 

Su ZZ, Lebedeva IV, Sarkar D, et al: Ionizing radiation enhances therapeutic activity of mda-7/IL-24: overcoming radiation and mda-7/IL-24-resistance in prostate cancer cells overexpressing the antiapoptotic proteins bcl-xL or bcl-2. Oncogene. 25:2339–2348. 2006. View Article : Google Scholar

24 

Park MA, Walker T, Martin AP, et al: MDA-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/CD95/PERK-dependent mechanism. Mol Cancer Ther. 8:1280–1291. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Eulitt PJ, Park MA, Hossein H, et al: Enhancing mda-7/IL-24 therapy in renal carcinoma cells by inhibiting multiple protective signaling pathways using sorafenib and by Ad.5/3 gene delivery. Cancer Biol Ther. 10:1290–1305. 2010. View Article : Google Scholar

26 

Ramesh R, Mhashilkar AM, Tanaka F, et al: Melanoma differentiation-associated gene 7/interleukin (IL)-24 is a novel ligand that regulates angiogenesis via the IL-22 receptor. Cancer Res. 63:5105–5113. 2003.

27 

Chada S, Mhashilkar AM, Liu Y, et al: mda-7 gene transfer sensitizes breast carcinoma cells to chemotherapy, biologic therapies and radiotherapy: correlation with expression of bcl-2 family members. Cancer Gene Ther. 13:490–502. 2006. View Article : Google Scholar

28 

Cosetti M, Yu GP and Schantz SP: Five-year survival rates and time trends of laryngeal cancer in the US population. Arch Otolaryngol Head Neck Surg. 134:370–379. 2008.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen X, Liu D, Wang J, Su Q, Zhou P, Liu J, Luan M and Xu X: Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells. Oncol Lett 7: 771-777, 2014.
APA
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J. ... Xu, X. (2014). Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells. Oncology Letters, 7, 771-777. https://doi.org/10.3892/ol.2014.1789
MLA
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J., Luan, M., Xu, X."Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells". Oncology Letters 7.3 (2014): 771-777.
Chicago
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J., Luan, M., Xu, X."Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells". Oncology Letters 7, no. 3 (2014): 771-777. https://doi.org/10.3892/ol.2014.1789
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Liu D, Wang J, Su Q, Zhou P, Liu J, Luan M and Xu X: Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells. Oncol Lett 7: 771-777, 2014.
APA
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J. ... Xu, X. (2014). Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells. Oncology Letters, 7, 771-777. https://doi.org/10.3892/ol.2014.1789
MLA
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J., Luan, M., Xu, X."Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells". Oncology Letters 7.3 (2014): 771-777.
Chicago
Chen, X., Liu, D., Wang, J., Su, Q., Zhou, P., Liu, J., Luan, M., Xu, X."Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells". Oncology Letters 7, no. 3 (2014): 771-777. https://doi.org/10.3892/ol.2014.1789
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team