Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells

Chen,Xuemei; Liu,Di; Wang,Junfu; Su,Qinghong; Zhou,Peng; Liu,Jinsheng; Luan,Meng; Xu,Xiaoqun

doi:10.3892/ol.2014.1789

Suppression effect of recombinant adenovirus vector containing hIL‑24 on Hep‑2 laryngeal carcinoma cells

Authors:
- Xuemei Chen
- Di Liu
- Junfu Wang
- Qinghong Su
- Peng Zhou
- Jinsheng Liu
- Meng Luan
- Xiaoqun Xu
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Affiliations: Department of Otolaryngology, The Second Affiliated Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China
Published online on: January 10, 2014 https://doi.org/10.3892/ol.2014.1789
Pages: 771-777

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Abstract

The melanoma differentiation‑associated gene‑7 [MDA‑7; renamed interleukin (IL)‑24] was isolated from human melanoma cells induced to terminally differentiate by treatment with interferon and mezerein. MDA‑7/IL‑24 functions as a multimodality anticancer agent, possessing proapoptotic, antiangiogenic and immunostimulatory properties. All these attributes make MDA‑7/IL‑24 an ideal candidate for cancer gene therapy. In the present study, the human MDA‑7/IL‑24 gene was transfected into the human laryngeal cancer Hep‑2 cell line and human umbilical vein endothelial cells (HUVECs) with a replication‑incompetent adenovirus vector. Reverse transcription polymerase chain reaction and western blot analysis confirmed that the Ad‑hIL‑24 was expressed in the two cells. The expression of the antiapoptotic gene, Bcl‑2, was significantly decreased and the IL‑24 receptor was markedly expressed in Hep‑2 cells following infection with Ad‑hIL‑24, but not in HUVECs. In addition, the expression of the proapoptotic gene, Bax, was induced and the expression of caspase‑3 was increased in the Hep‑2 cells and HUVECs. Methyl thiazolyl tetrazolium assay indicated that Ad‑hIL‑24 may induce growth suppression in Hep‑2 cells but not in HUVECs. In conclusion, Ad‑hIL‑24 selectively inhibits proliferation and induces apoptosis in Hep‑2 cells. No visible damage was found in HUVECs. Therefore, the results of the current study indicated that Ad‑hIL‑24 may have a potent suppressive effect on human laryngeal carcinoma cell lines, but is safe for healthy cells.

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