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2014-March Volume 7 Issue 3

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Article

Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration

  • Authors:
    • Jun Yao
    • Cui‑Juan Qian
    • Bei Ye
    • Zhi‑Qiang Zhao
    • Jie Wei
    • Yong Liang
    • Xin Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China, Department of Gastroenterology, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China
  • Pages: 902-908
    |
    Published online on: January 15, 2014
       https://doi.org/10.3892/ol.2014.1804
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Abstract

Fascin protein plays important roles in tumor metastasis and is prognostically relevant to human gastric cancer (GC). However, its role in the development and progression of GC has not been comprehensively investigated. In the present study, results revealed that upregulation of fascin by interleukin‑6 promotes GC cell migration and invasion in a signal transducer and activator of transcription 3 (STAT3)‑dependent manner in MKN45 cells. Furthermore, STAT3 directly regulated fascin expression and nuclear factor‑κB (NF‑κB) bound to the fascin promoter in a STAT3‑dependent and Notch‑independent manner. Therefore, results demonstrate that STAT3 and NF‑κB are required for upregulation of fascin and for cell migration and invasion in MKN45 cells. Effects of the treatments on cell signaling were detected by qPCR, western blot analysis and chromatin immunoprecipitation (ChIP) assay. Cell migration and invasion were analyzed using in vitro scratch wound healing assay, transwell and Matrigel assays, and xenograft model. In addition, the STAT3‑NF‑κB‑fascin signaling axis is identified as a therapeutic target for blocking GC cell invasion and migration.
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1 

Hartgrink HH, Jansen EP, van Grieken NC and van de Velde CJ: Gastric cancer. Lancet. 374:477–490. 2009. View Article : Google Scholar

2 

Pasini F, Fraccon AP and Manzoni DEG: The role of chemotherapy in metastatic gastric cancer. Anticancer Res. 31:3543–3554. 2011.PubMed/NCBI

3 

Valastyan S and Weinberg RA: Tumor metastasis: molecular insights and evolving paradigms. Cell. 147:275–292. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Smith SC and Theodorescu D: Learning therapeutic lessons from metastasis suppressor proteins. Nat Rev Cancer. 9:253–264. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Hashimoto Y, Skacel M and Adams JC: Roles of fascin in human carcinoma motility and signaling: prospects for a novel biomarker? Int J Biochem Cell Biol. 37:1787–1804. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Chen L, Yang S, Jakoncic J, Zhang JJ and Huang XY: Migrastatin analogues target fascin to block tumour metastasis. Nature. 464:1062–1066. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Hashimoto Y, Kim DJ and Adams JC: The roles of fascins in health and disease. J Pathol. 224:289–300. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Hashimoto Y, Shimada Y, Kawamura J, Yamasaki S and Imamura M: The prognostic relevance of fascin expression in human gastric carcinoma. Oncology. 67:262–270. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Okamoto W, Okamoto I, Arao T, Yanagihara K, Nishio K and Nakagawa K: Differential roles of STAT3 depending on the mechanism of STAT3 activation in gastric cancer cells. Br J Cancer. 105:407–412. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Woo S, Lee BL, Yoon J, et al: Constitutive activation of signal transducers and activators of transcription 3 correlates with better prognosis, cell proliferation and hypoxia-inducible factor-1α in human gastric cancer. Pathobiology. 78:295–301. 2011.PubMed/NCBI

11 

Mejías-Luque R, Peiró S, Vincent A, Van Seuningen I and de Bolós C: IL-6 induces MUC4 expression through gp130/STAT3 pathway in gastric cancer cell lines. Biochim Biophys Acta. 1783:1728–1736. 2008.PubMed/NCBI

12 

Lee MJ, Lee JK, Choi JW, et al: Interleukin-6 induces S100A9 expression in colonic epithelial cells through STAT3 activation in experimental ulcerative colitis. PLoS One. 7:e388012012. View Article : Google Scholar : PubMed/NCBI

13 

Zhang L, Yang J, Qian J, et al: Role of the microenvironment in mantle cell lymphoma: IL-6 is an important survival factor for the tumor cells. Blood. 120:3783–3792. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Chen J, Wang J, Lin L, et al: Inhibition of STAT3 signaling pathway by nitidine chloride suppressed the angiogenesis and growth of human gastric cancer. Mol Cancer Ther. 11:277–287. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Liu Q, Li G, Li R, et al: IL-6 promotion of glioblastoma cell invasion and angiogenesis in U251 and T98G cell lines. J Neurooncol. 100:165–176. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Snyder M, Huang XY and Zhang JJ: Signal transducers and activators of transcription 3 (STAT3) directly regulates cytokine-induced fascin expression and is required for breast cancer cell migration. J Biol Chem. 286:38886–38893. 2011. View Article : Google Scholar

17 

Snyder M, Huang XY and Zhang JJ: Stat3 is essential for neuronal differentiation through direct transcriptional regulation of the Sox6 gene. FEBS Lett. 585:148–152. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Snyder M, Huang XY and Zhang JJ: Stat3 directly controls the expression of Tbx5, Nkx2.5, and GATA4 and is essential for cardiomyocyte differentiation of P19CL6 cells. J Biol Chem. 285:23639–23646. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Sun J, He H, Xiong Y, et al: Fascin protein is critical for transforming growth factor β protein-induced invasion and filopodia formation in spindle-shaped tumor cells. J Biol Chem. 286:38865–38875. 2011.

20 

Fu H, Wen JF, Hu ZL, Luo GQ and Ren HZ: Knockdown of fascin1 expression suppresses the proliferation and metastasis of gastric cancer cells. Pathology. 41:655–660. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Lee SA, Choi SR, Jang JS, et al: Expression of VEGF, EGFR, and IL-6 in gastric adenomas and adenocarcinomas by endoscopic submucosal dissection. Dig Dis Sci. 55:1955–1963. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Giraud AS, Menheniott TR and Judd LM: Targeting STAT3 in gastric cancer. Expert Opin Ther Targets. 16:889–901. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Bollrath J and Greten FR: IKK/NF-kappaB and STAT3 pathways: central signalling hubs in inflammation-mediated tumour promotion and metastasis. EMBO Rep. 10:1314–1319. 2009. View Article : Google Scholar

24 

Jiang P, Enomoto A and Takahashi M: Cell biology of the movement of breast cancer cells: intracellular signalling and the actin cytoskeleton. Cancer Lett. 284:122–130. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Fink K and Boratyński J: The role of metalloproteinases in modification of extracellular matrix in invasive tumor growth, metastasis and angiogenesis. Postepy Hig Med Dosw (Online). 66:609–628. 2012.(In Polish).

26 

Al-Alwan M, Olabi S, Ghebeh H, et al: Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One. 6:e273392011. View Article : Google Scholar : PubMed/NCBI

27 

Kotipatruni RR, Nalla AK, Asuthkar S, Gondi CS, Dinh DH and Rao JS: Apoptosis induced by knockdown of uPAR and MMP-9 is mediated by inactivation of EGFR/STAT3 signaling in medulloblastoma. PLoS One. 7:e447982012. View Article : Google Scholar : PubMed/NCBI

28 

Sansone P and Bromberg J: Targeting the interleukin-6/Jak/stat pathway in human malignancies. J Clin Oncol. 30:1005–1014. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Senft C, Priester M, Polacin M, Schröder K, Seifert V, Kögel D and Weissenberger J: Inhibition of the JAK-2/STAT3 signaling pathway impedes the migratory and invasive potential of human glioblastoma cells. J Neurooncol. 101:393–403. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Wu WK, Cho CH, Lee CW, Fan D, Wu K, Yu J and Sung JJ: Dysregulation of cellular signaling in gastric cancer. Cancer Lett. 295:144–153. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Hsu KW, Hsieh RH, Huang KH, et al: Activation of the Notch1/STAT3/Twist signaling axis promotes gastric cancer progression. Carcinogenesis. 33:1459–1467. 2012. View Article : Google Scholar

32 

Yang J, Liao X, Agarwal MK, Barnes L, Auron PE and Stark GR: Unphosphorylated STAT3 accumulates in response to IL-6 and activates transcription by binding to NFkappaB. Genes Dev. 21:1396–1408. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Kress AK, Kalmer M, Rowan AG, Grassmann R and Fleckenstein B: The tumor marker Fascin is strongly induced by the Tax oncoprotein of HTLV-1 through NF-kappaB signals. Blood. 117:3609–3612. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Wu Y and Zhou BP: TNF-alpha/NF-kappaB/Snail pathway in cancer cell migration and invasion. Br J Cancer. 102:639–644. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao J, Qian CJ, Ye B, Zhao ZQ, Wei J, Liang Y and Zhang X: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration. Oncol Lett 7: 902-908, 2014.
APA
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., & Zhang, X. (2014). Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration. Oncology Letters, 7, 902-908. https://doi.org/10.3892/ol.2014.1804
MLA
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., Zhang, X."Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration". Oncology Letters 7.3 (2014): 902-908.
Chicago
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., Zhang, X."Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration". Oncology Letters 7, no. 3 (2014): 902-908. https://doi.org/10.3892/ol.2014.1804
Copy and paste a formatted citation
x
Spandidos Publications style
Yao J, Qian CJ, Ye B, Zhao ZQ, Wei J, Liang Y and Zhang X: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration. Oncol Lett 7: 902-908, 2014.
APA
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., & Zhang, X. (2014). Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration. Oncology Letters, 7, 902-908. https://doi.org/10.3892/ol.2014.1804
MLA
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., Zhang, X."Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration". Oncology Letters 7.3 (2014): 902-908.
Chicago
Yao, J., Qian, C., Ye, B., Zhao, Z., Wei, J., Liang, Y., Zhang, X."Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor‑κB in gastric cancer: Implications in cell invasion and migration". Oncology Letters 7, no. 3 (2014): 902-908. https://doi.org/10.3892/ol.2014.1804
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