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Article

BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition

  • Authors:
    • Onica Legendre
    • Ayisha Sookdeo
    • David A. Foster
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, Hunter College of The City University of New York, New York, NY 10065, USA
  • Pages: 1165-1168
    |
    Published online on: January 28, 2014
       https://doi.org/10.3892/ol.2014.1833
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Abstract

Smad4 is a critical regulator of transforming growth factor (TGF)‑β signaling and is defective in numerous human cancers. In total, 30% of pancreatic cancers harbor a homozygous deletion of Smad4. The human pancreatic cancer cell line, BxPC3, has been reported to be Smad4‑null due to a homozygous deletion and has been widely used as a Smad4‑null model. The present study reports that Smad4 DNA is present in BxPC3 cells, and under conditions of suppressed mammalian target of rapamycin complex 1 (mTORC1) and phosphatidylinositol‑3‑kinase, a truncated Smad4 protein is expressed. While a high level of Smad4 protein can be expressed in these cells, the cells do not respond to TGF‑β. The Smad4 defect in BxPC3 cells likely occurs via translocation rather than deletion as previously reported.
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Copy and paste a formatted citation
Spandidos Publications style
Legendre O, Sookdeo A and Foster DA: BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition. Oncol Lett 7: 1165-1168, 2014.
APA
Legendre, O., Sookdeo, A., & Foster, D.A. (2014). BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition. Oncology Letters, 7, 1165-1168. https://doi.org/10.3892/ol.2014.1833
MLA
Legendre, O., Sookdeo, A., Foster, D. A."BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition". Oncology Letters 7.4 (2014): 1165-1168.
Chicago
Legendre, O., Sookdeo, A., Foster, D. A."BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition". Oncology Letters 7, no. 4 (2014): 1165-1168. https://doi.org/10.3892/ol.2014.1833
Copy and paste a formatted citation
x
Spandidos Publications style
Legendre O, Sookdeo A and Foster DA: BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition. Oncol Lett 7: 1165-1168, 2014.
APA
Legendre, O., Sookdeo, A., & Foster, D.A. (2014). BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition. Oncology Letters, 7, 1165-1168. https://doi.org/10.3892/ol.2014.1833
MLA
Legendre, O., Sookdeo, A., Foster, D. A."BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition". Oncology Letters 7.4 (2014): 1165-1168.
Chicago
Legendre, O., Sookdeo, A., Foster, D. A."BxPC3 pancreatic cancer cells express a truncated Smad4 protein upon PI3K and mTOR inhibition". Oncology Letters 7, no. 4 (2014): 1165-1168. https://doi.org/10.3892/ol.2014.1833
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